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Using transient transfected Chinese hamster ovary (CHO) cells by pET28a-LIC-NTF4 in gene therapy
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Mohammad Hassan Karimfar   , Reza Mastery Farahani , Mohsen Noorozian , Maryam Sadat Khoramgah , Ali Asghar Keramati Nia , Ardeshir Moayeri , Hadi Azimi , Hojjat-Allah Abbaszadeh |
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Abstract: (4379 Views) |
| Introduction: Neurotrophins, as a family of proteins, are responsible for induction of the survival, development, and function of neurons. Also, neurotropic factors are growth factors like Neurotrophins that help neurons survive. Moreover, Neurotrophins differentiate between progenitor cells so that neurons are formed. Despite the fact that the majority of mammalian brain neurons are produced prenatally, the capability of growing new neurons from neural stem cells will be preserved by parts of the adult brain. This process is known as neurogenesis. Neurogenesis is stimulated and controlled by neurotrophins.
Materials and methods: The recombinant plasmid transformed to E.coli ´ cell and colonies that contain plasmid were selected by Colony PCR. Enzyme digestion and sequencing were monitored to approve the accuracy of extracted plasmid of the clones.
Results: Plasmid was verified correctly. Based on RT-PCR and western blotting, the transcription of NTF4 gene and the expression of NTF4 protein after transfection were proved.
Conclusion: Plasmid was correctly constructed, CHO Cells were successfully transfected by transfection, and protein could be properly expressed. The results provided a solid foundation for the studies in the area of the transplantation of gene-modified CHO Cells to further spinal cord regeneration. |
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| Keywords: Transfection, Cloning, Molecular, Neurotrophins 4, Genetic Vectors |
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Full-Text [PDF 722 kb]
(1342 Downloads)
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Type of Study: Research |
Subject:
Medical Anatomy
Received: 2015/02/27 | Accepted: 2015/03/2 | Published: 2015/03/7
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