Journal of Basic Research in Medical Sciences
مجله ی تحقیقات پایه در علوم پزشکی
Journal of Basic Research in Medical Sciences
Medical Sciences
http://jbrms.medilam.ac.ir
1
admin
2383-0506
2383-0972
doi
en
jalali
1395
6
1
gregorian
2016
9
1
3
4
online
1
fulltext
en
Purity determining of cultured OECs from olfactory mucosa of rats' pups
Medical Anatomy
Medical Anatomy
پژوهشي
Research
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<p style="text-align: justify;"><strong>Introduction:</strong> Cell transplantation is one of the main strategies for spinal cord injury repair. As OECs of the olfactory mucosa can be obtained by simple biopsy in all individuals without affecting their smell sensation, OECs considered as a promising candidate for autologous transplantation in the nervous system injury, especially for spinal cord repair. Thereby in the current study OECs were cultured from olfactory mucosa of 7 days old rats' pups and their purity was examined by flow-cytometry after simultaneous double staining for p75 and GFAP markers.</p>
<p style="text-align: justify;"><strong>Materials and methods:</strong> 7 days old Wistar rats' pups were deeply anesthetized by ketamine / xylazine (60/6mg/Kg). Then the nasal cavity was opened sagittally and the olfactory mucosa was separated from posterior part of nasal septum and at last OECs were obtained from lamina properia of olfactory mucosa and were cultured. The cultured cells were simultaneously immunolabeled for p75 and GFAP markers and finally purity of cultured cells assessed by flow-cytometry.</p>
<p style="text-align: justify;"><strong>Results:</strong> cultured OECs demonstrated two different morphologies, a spindle shape Schwann-like and an astrocyte-like OECs with flat sheet-like morphology. In addition, simultaneous immunolabeling for p75 and GFAP markers of OECs exhibited OECs were positive for both markers at the same time. The flow-cytometry results displayed that 87.9±2.4% of cells were p75/ GFAP double positive cells,1.05±0.4 only p75 positive and 5.8±1.5% were single positive for GFAP.</p>
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<p dir="ltr" style="text-align: justify;"><strong>Conclusion:</strong> Purity of cultured OECs in our study is probably more than 87.9% by flow- owing to p75+/S100+ and GFAP+/S100+ olfactory ensheathing cells were not counted. Thus the culture procedure of this study seems to be a good protocol for OECs purifying and cell therapy in CNS damages.</p>
OECs, Cell therapy, Spinal cord injury, Flowcytometry
12
21
http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-93-1&slc_lang=en&sid=1
Monireh
Azizi
azizi.moaz@gmail.com
10031947532846002253
10031947532846002253
No
Department of Anatomy, Ilam University of Medical Sciences, Ilam , Iran
Mehrdad
Bakhtiari
mt.joghataei@yahoo.com
10031947532846002254
10031947532846002254
No
Molecular and Cellular Research Center, Iran University of Medical Sciences, Tehran, Iran
Farhid
Farahmand Ghavi
bakhtiari.m@iums.ac.ir
10031947532846002255
10031947532846002255
No
Novel Drug Delivery Systems Department, Iran Polymer and Petrochemical Institute, Tehran, Iran
Mojgan
Zandi
ffarahmandghavi@yahoo.com
10031947532846002256
10031947532846002256
No
Biomaterials Department, Iran Polymer and Petrochemical Institute, Tehran, Iran
Mohammad
Imani
moayeri46@yahoo.com
10031947532846002257
10031947532846002257
No
Novel Drug Delivery Systems Department, Iran Polymer and Petrochemical Institute, Tehran, Iran
Mohammad Taghi
Joghataei
mt.joghataei @yahoo.com
10031947532846002258
10031947532846002258
Yes
Molecular and Cellular Research Center, Iran University of Medical Sciences, Tehran, Iran