Journal of Basic Research in Medical Sciences
مجله ی تحقیقات پایه در علوم پزشکی
Journal of Basic Research in Medical Sciences
Medical Sciences
http://jbrms.medilam.ac.ir
1
admin
2383-0506
2383-0972
doi
en
jalali
1395
3
1
gregorian
2016
6
1
3
3
online
1
fulltext
en
Determination of vancomycin and methicillin resistance in clinical isolates of Staphylococcus aureus in hospitals of Ilam city
Microbiology
Microbiology
پژوهشي
Research
<div dir="ltr">
<p style="text-align: justify;"><strong>Introduction: </strong>In this study, using the phenotypic and genotypic methods, oxacillin susceptibility in <em>Staphylococcus aureus</em> (<em>S. aureus</em>) strains isolated from patients at two government hospitals in Ilam, Iran was tested.</p>
<p style="text-align: justify;"><strong>Materials and methods:</strong> Out of 200 <em>S. aureus</em> isolates from different human clinical specimens consisting of blood (31%), wound (20%), urine (21%), catheters (7%), sputum (12%), others (9%) were collected. The methicillin resistant <em>S. aureus</em> isolates were investigated using disk diffusion methods and oxacillin (1μg) and cefoxitin (30μg), on Mueller-Hinton agar were used, and <em>MecA</em> and <em>vanA</em> genes were detected by PCR. In addition, the isolates were tested for their antibiogram profiles.</p>
<p style="text-align: justify;"><strong>Results: </strong>Among 200 <em>S. aureus</em> strains included in this study, 35.96% were MRSA. The percentage of resistance by disk diffusion method was as below: penicillin 85.96%, vancomycin 0%, ampicillin 87.71%, gentamicin 48.25% erythromycin 54.25%, clindamycin 32.45%, amikacin 21.05%, ciprofloxacin 42.10%, tetracycline 51.75% and co-trimoxazole 42.10%. Phenotyping method by disk diffusion method using oxacillin and cefoxitin for detecting of MRSA showed sensitivity and specificity of about 33.33% and 35.96%, respectively. Presence of <em>MecA</em> and <em>vanA</em> genes in MRSA isolates by PCR were 35.96% and 0%, respectively. The oxacillin and cefoxitin disk diffusion methods showed 92.68% and 100% sensitivity, respectively, and 98.8% specificity.</p>
<p style="text-align: justify;"><strong>Conclusion:</strong> Our finding showed that, the cefoxitin disk diffusion method is better in compared to the oxacillin disk diffusion similar to results from detecting of <em>MecA</em> gene in PCR as a golden test.</p>
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Staphylococcus aureus, MecA, Methicillin, Vancomycin
1
6
http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-136-1&slc_lang=en&sid=1
Arman
Rostamzad
arostamzad381@yahoo.com
10031947532846001894
10031947532846001894
Yes
Department of Biology Faculty of Sciences, Ilam University, Ilam, Iran
Nabi
Rostamneia
nabi.biology@gmail.com
10031947532846001895
10031947532846001895
No
Department of Biology Faculty of Sciences, Ilam University, Ilam, Iran
Fazel
Pourahmad
f.pourahmad@ilam.ac.ir
10031947532846001896
10031947532846001896
No
School of Veterinary Medicine, Ilam University, Ilam, Iran
Morteza
Shamsi
arostamzad381@yahoo.com
10031947532846001897
10031947532846001897
No
Department of Parasitology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran