Journal of Basic Research in Medical Sciences
مجله ی تحقیقات پایه در علوم پزشکی
Journal of Basic Research in Medical Sciences
Medical Sciences
http://jbrms.medilam.ac.ir
1
admin
2383-0506
2383-0972
doi
en
jalali
1396
12
1
gregorian
2018
3
1
5
2
online
1
fulltext
en
Time dependent of epigenetic effect of disulfiram on tumor suppressor gene of RASSF1A in Hela cancer cell line
Genetics
Genetics
پژوهشي
Research
<div><strong>Introduction:</strong> Cervical cancer is the third most common tumor among women. Surgery, radiotherapy, and chemotherapy are common treatments, however high stage tumors have frequently poor prognosis. Nowadays, the epigenetic reversion introduced as an efficient strategy of treatment of cervical cancer. In the process, inhibitors of DNA methyltransferase (DNMT) induce re-expression of tumor suppressor genes. Among these inhibitors, disulfiram (DSF) has been suggested as non-nucleoside analogous. In this research, we evaluated the epigenetic effect of DSF on demethylation of the tumor suppressor gene, RASSF1A, in Hela cell line.<br>
<strong>Materials and methods: </strong>Hela cells were cultured and treated with different doses from 2.5 to 37.5µM during 24, 48 and 72 hours. MTT assay was carried out to find half maximal inhibitory concentration (IC<sub>50</sub>). The methylation specific PCR (MSP) assay was applied to evaluate methylation pattern.<br>
<strong>Results: </strong>The IC<sub>50</sub> of DSF was determined at the 2.5, 12.5, and 15µM after72 hours. The MSP results showed partial demethylation at mentioned concentrations after 72h but unmethylated band was not observed after 24h.<br>
<strong>Conclusion: </strong>Our findings indicated that, IC<sub>50</sub> of DSF exerted a biphasic effect in Hela cell line and at least 72 hours treatment is needed for the epigenetic reversion of DSF on RASSF1Ain Hela cell line.</div>
Disulfiram, RASSF1A, Epigenetic reversion, Methylation, Cervical cancer
8
13
http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-303-1&slc_lang=en&sid=1
Seyedeh naghme
Noorirad
naghmenoorirad@gmail.com
10031947532846002831
10031947532846002831
No
Department of Anatomical science, Faculty of Medicine, Babol University of Medical Sciences
Mohsen
Pourghasem
Mpourghasem@hotmail.com
10031947532846002832
10031947532846002832
No
Department of Anatomical science, Faculty of Medicine, Babol University of Medical Sciences
Farideh
Feizi
faridehfeizi@yahoo.com
10031947532846002833
10031947532846002833
No
Department of Anatomical science, Faculty of Medicine, Babol University of Medical Sciences
Zeinab
Abedian
zeinab_abedian@yahoo.com
10031947532846002834
10031947532846002834
No
Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
Masoumeh
Ghasemi
MSm.ghasemi@gmail.com
10031947532846002835
10031947532846002835
No
Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
Zahra
Babazadeh
zbabazadeh@gmail.com
10031947532846002836
10031947532846002836
Yes
Department of Anatomical Sciences, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran
Navid
Rabiee
10031947532846002837
10031947532846002837
No
Department of Chemistry, Shahid Beheshti University, Tehran, Iran