Journal of Basic Research in Medical Sciences
مجله ی تحقیقات پایه در علوم پزشکی
Journal of Basic Research in Medical Sciences
Medical Sciences
http://jbrms.medilam.ac.ir
1
admin
2383-0506
2383-0972
doi
en
jalali
1396
12
1
gregorian
2018
3
1
5
2
online
1
fulltext
en
The investigation of antibiotic resistance and rapid detection of group B Streptococcus (Bca) from vaginal specimens of pregnant women by colony PCR method
Microbiology
Microbiology
پژوهشي
Research
<strong>Introduction:</strong> Group B<em> Streptococcus </em>(GBS) is one of the most causes of neonatal infections. The bacterium colonizes genitourinary tracts of pregnant women and transmits to infants. The aim of this study was investigating colony PCR and culture methods to detection of GBS in pregnant women.<br>
<strong>Materials and methods:</strong> Hundred pregnant women, at the 35<sup>th</sup> and 37<sup>th</sup> weeks of pregnancy, were selected from the Obstetrics and Gynecology Unit of the Moatazedi and Shahid Chamran Hospitals in Kermanshah province. Specimens were collected from vaginal introitus and investigated by selective culture and colony PCR methods. Then, antibiotic resistance tests were performed according to the latest guidelines of Clinical and Laboratory Standards Institute (CLSI).<br>
<strong>Results:</strong> Prevalence of GBS colonization was shown to be 5% and 6% by the culture and colony PCR methods, respectively. Also, resistance rate to erythromycin, penicillin, vancomycin, and the clindamycin were determined to be 50%, 16.66%, 16.66% and 33.33 %, respectively. Moreover, the highest resistance was for erythromycin and the appropriate antibiotics were penicillin and then vancomycin.<br>
<strong>Conclusion:</strong> A higher prevalence of GBS colonization in pregnant women in the Kermanshah city of Iran was detected using colony PCR method compared to culture method.
Antibiotic Resistance, BCA gene, Colony-PCR, GBS
27
32
http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-168-5&slc_lang=en&sid=1
Aazam Khani
Daramroodi
gol.keshavarzi@gmail.com
10031947532846003004
10031947532846003004
No
Department of Biology, Kurdistan Science and Research Branch, Islamic Azad University, Sanandaj, Iran
Fatemeh
Keshavarzi
gol.keshavarzi@gmail.com
10031947532846003005
10031947532846003005
Yes
Department of Genetics, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran
Farshid
Raissi
gol.keshavarzi@gmail.com
10031947532846003006
10031947532846003006
No
Department of Pathology, Faculty of Medicine, Kermanshah University of Medical Science, Kermanshah, Iran