Journal of Basic Research in Medical Sciences
مجله ی تحقیقات پایه در علوم پزشکی
Journal of Basic Research in Medical Sciences
Medical Sciences
http://jbrms.medilam.ac.ir
1
admin
2383-0506
2383-0972
doi
en
jalali
1398
3
1
gregorian
2019
6
1
6
3
online
1
fulltext
en
Up-regulation of circulating miR-93-5p in patients with relapsing-remitting multiple sclerosis
Genetics
Genetics
پژوهشي
Research
<div><strong>Introduction: </strong>Multiple sclerosis (MS) is an inflammatory demyelinating in which there is no standardized method to detect this disease activity. It has shown abnormal microRNAs (miRNAs) function in peripheral blood immune cells. miRNAs expression is probably responsible for immunological features associated with MS. The purpose of the current study was to investigate the association of miR-93-5p expression with MS disease.<br>
<strong>Materials and methods: </strong>In this case-control study, a totally of 30 relapsing-remitting MS (RRMS) patients and 30 healthy subjects were enrolled. Following miRNA extraction and cDNA synthesis, miR-93-5p expression was examined in peripheral blood mononuclear cells (PBMCs) using real-time polymerase chain reaction.<br>
<strong>Results: </strong>The expression of miR-93-5p was significantly increased in RRMS patients compared to healthy subjects (P=0.001). Receiver operating curve (ROC) analysis identified a strong predictive power of miR‑93-5p on discriminating MS from healthy individuals, with the area under the curve (AUC) of 0.939 (95% CI; 0.8581-1.000). On the other hand, the samples were analyzed based on the type of drug treatment (interferon and non-interferon), which did not show any considerable differences (P=0.863).<br>
<strong>Conclusion: </strong>Our findings showed that <em>miR-93-5p</em> has highly elevated expression in patients with RRMS compared to healthy subjects. Based on the results <em>miR-93-5p</em> may be a prospective biomarker with the potential use for diagnosis of RRMS patients.</div>
Multiple sclerosis, MicroRNA, miR-93-5p
4
11
http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-438-1&slc_lang=en&sid=1
Jalil
Shafiei
jalilshafiei20@gmail.com
10031947532846003373
10031947532846003373
No
Department of Genetic, Science and Research Branch, Islamic Azad University, Tehran, Iran
Gholamreza
Javadi
gh.vadi@yahoo.com
10031947532846003374
10031947532846003374
No
Department of Genetic, Science and Research Branch, Islamic Azad University, Tehran, Iran
Behnaz
Nateghi
behnaz.nateqi@gmail.com
10031947532846003375
10031947532846003375
No
Department of Biochemistry, Faculty of Science, Nourdanesh Institutions of Higher Education, Meimeh, Isfahan, Iran
Vahid
Shaygannejad
vahid.shaygannejad@yahoo.com
10031947532846003376
10031947532846003376
No
Department of Neurology, Isfahan Neurosciences Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
Mansoor
Salehi
M_salehi@med.mui.ac.ir
10031947532846003377
10031947532846003377
Yes
Department of Genetics and Molecular Biology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran