Journal of Basic Research in Medical Sciences
مجله ی تحقیقات پایه در علوم پزشکی
Journal of Basic Research in Medical Sciences
Medical Sciences
http://jbrms.medilam.ac.ir
1
admin
2383-0506
2383-0972
doi
en
jalali
1398
6
1
gregorian
2019
9
1
6
4
online
1
fulltext
en
The effects of endurance training and pumpkin seed consumption on oxidative stress and DNA damage markers in the cardiac muscle of rats poisoned with H2O2
Physical medicine
Physical medicine
پژوهشي
Research
<div><strong>Introduction: </strong>Oxidative stress can impair the antioxidant protective capacity of the body, which is associated with decreased capacity of the body's internal defense system. There are some evidences that training and pumpkin seed (PS) consumption by different mechanisms have a protective effect on myocardial tissue by inhibiting oxidative stress. The aim of the present study was to investigate the effects of endurance training (ET) and PS consumption on oxidative stress and DNA damage markers in the cardiac tissue of rats poisoned with H<sub>2</sub>O<sub>2</sub>.<br>
<strong>Materials and methods: </strong>In this experimental study, 42 healthy male Wistar rats were randomly divided into 7 groups, including: 1) control, 2) H<sub>2</sub>O<sub>2</sub>, 3) H<sub>2</sub>O<sub>2</sub>+ET, 4) H<sub>2</sub>O<sub>2</sub>+1mg/kg PS, 5) H<sub>2</sub>O<sub>2</sub>+2 mg/kg PS, 6) H<sub>2</sub>O<sub>2</sub>+ET+ 1 mg/kg PS, and 7) H<sub>2</sub>O<sub>2</sub>+ET+2 mg/kg PS. During eight weeks, groups 2 to 7 received 1 mg/kg of H<sub>2</sub>O<sub>2</sub> (manufactured by Sigma Aldrich Co.) peritoneally; also, groups 3, 6, and 7 ran for 30 minutes on a treadmill at a speed of 23 m/min for 5 sessions per week. Independent sample t-test and two-way ANOVA with Bonferroni’s <em>post- hoc</em> tests were used to analyze the data (P<0.05).<br>
<strong>Results: </strong>H<sub>2</sub>O<sub>2</sub>poisoning significantly decreased ATP and increased cytochrome-C, MDA and PAB (P=0.001); on the other hand, ET and PS consumption alone significantly increased ATP and decreased cytochrome-C and MDA (P=0.001); but the interactive effects of ET and PS consumption on increase of ATP and decrease of cytochrome-C, MDA and PAB were not significant (P>0.05). In addition, although consumption of 1 mg/kg PS had no significant effect on increase of ATP and decrease of cytochrome-C and MDA (P>0.05), 2 mg/kg PS significantly increased ATP and decreased cytochrome-C, MDA and PAB (P<0.05).<br>
<strong>Conclusion: </strong>It seems that ET and PS consumption alone can be a good strategy to reduce the adverse effects of toxicity by inducing oxidative stress; however the effects of PS are dose-dependent.</div>
Training, Pumpkin Seed, Oxidative stress, Heart, H2O2
12
19
http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-447-1&slc_lang=en&sid=1
Mahshid
Mohazzab
mahshidm60l@yahoo.com
10031947532846003468
10031947532846003468
No
Department of Sport Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
Hasan
Matinhomaee
hasanmatinhomaee@gmail.com
10031947532846003469
10031947532846003469
Yes
Department of Sport Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
Ali
Hosseini
alihoseini_57@yahoo.com
10031947532846003470
10031947532846003470
No
Department of Sport Physiology, Marvdasht Branch, Islamic Azad University, Marvdasht, Iran
Saleh
Rahmati Ahmad Abad
alihoseini_57@yahoo.com
10031947532846003471
10031947532846003471
No
Department of Physical Education, Pardis Branch, Islamic Azad University, Pardis, Iran