Journal of Basic Research in Medical Sciences
مجله ی تحقیقات پایه در علوم پزشکی
Journal of Basic Research in Medical Sciences
Medical Sciences
http://jbrms.medilam.ac.ir
1
admin
2383-0506
2383-0972
doi
en
jalali
1398
12
1
gregorian
2020
3
1
7
2
online
1
fulltext
en
Influence of Smyrnium cordifolium Boiss extract on the hippocampus Mu-opioid receptors in Mice
Pharmaceutical Biotechnology
Pharmaceutical Biotechnology
پژوهشي
Research
<div><strong>Introduction: </strong>Agonists of the Mu-opioid receptors (MOR), such as morphine are lengthily used for the treatment of moderate to pain, depression and anxiety. But the dose involved achieving adequate pain relief often elicits multiple unwanted side effects, including addiction and tolerance. Opioids produce their actions at a cellular level by activating MOR. These receptors are distributed throughout the central nervous system (CNS). The target of this study was the effect of <em>Smyrnium cordifolium</em> extract (SCE) on the hippocampus Cornu Ammonis 1 (CA1) aria MOR compared to clonidine.<span dir="RTL"></span><br>
<strong>Materials and methods: </strong>Extract of the aerial parts <em>S.cordifolium</em> was extracted by Soxhlet method. Addiction was created using the subcutaneous injections of morphine for 7 days. To evaluate the effects of SCE, the mice were divided up 5 groups. The first group (Control) received just morphine. The 2th group received morphine and Clonidine (0.2mg/kg). Groups 3, 4 and 5 were treated morphine and SCE (E1, E2 and E3). In all groups, on the seventh day 30 min after naloxone injection, their brains were perfusion with formaldehyde and removed for immunohistochemical investigation. <strong><span dir="RTL"></span></strong><br>
<strong>Results: </strong>The present immunohistochemical of the CA1 hippocampus study showed that group E1, there is a significant difference in MOR optical density compared to the control group at the level (P<0.05) and relative to the CLO group at the level (P<0.001). However, in groups E2, and E3 the MOR optical density increased compared to the control group and had a significant difference in level (P<0.001) and did not have a significant difference compared to the CLO group.<br>
<strong>Conclusion: </strong>The study showed that with increasing <em>S.cordifolium</em> extract concentration, the optical density of MOR in the hippocampus increased, and this increase was dose-dependent. This increase in the CA1 hippocampus MOR optical density may be due to endocytosis or desensitization of MOR in neurons.</div>
Hippocampus, Mu-opioid receptor, Mice, Smyrnium cordifolium
29
37
http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-87-1&slc_lang=en&sid=1
Pari
Nazari
Nazp14st@Gmail.com
10031947532846003836
10031947532846003836
No
Islamic Azad University
Parichehreh
Yaghmaei
Yaghmaei_p@yahoo.com
10031947532846003837
10031947532846003837
Yes
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Alireza
Rangin
alireza1121sar@gmail.com
10031947532846003838
10031947532846003838
No
Islamic Azad University
Naser
Abbasi
Abbasi-n@medilam.ac.ir
10031947532846003839
10031947532846003839
No
Department of Pharmacology, Ilam University of Medical Sciences, Ilam, Iran