Journal of Basic Research in Medical Sciences
مجله ی تحقیقات پایه در علوم پزشکی
Journal of Basic Research in Medical Sciences
Medical Sciences
http://jbrms.medilam.ac.ir
1
admin
2383-0506
2383-0972
doi
en
jalali
1399
6
1
gregorian
2020
9
1
7
4
online
1
fulltext
en
Association of ESR-α and ESR-β gene polymorphisms with implantation failure in IVF-treated women in northwest of Iran: A case-control study
Genetics
Genetics
پژوهشي
Research
<div><strong>Introduction:</strong> Estrogen, a crucial hormone during pregnancy, acts through two types of receptors. The estrogen receptor alpha and beta (ESR-α and ESR-β) are more abundant and exists in all human reproductive systems. Association of ESR-α and ESR-β genes polymorphisms has been reported in some reproductive problems such as spontaneous abortion, endometriosis-related infertility, and <em>in vitro</em> fertilization failure. In the present study, we investigated association between single nucleotide polymorphisms rs9340799 and rs2234693 (ESR-α) and rs1256049 and rs4986938 (ESR-β) with implantation failure in Iranian women.<br>
<strong>Materials and Methods:</strong> In this case-control study, we collected 60 women with implantation failure as case group and 60 age and ethnically matched IVF-treated women with successful implantation as controls. Extraction of genomic DNA of both case and control members was performed using salting out method. The case and control groups genotyping was performed using tetra-primer amplification refractory mutation system-polymerase chain reaction (Tetra-ARMS PCR) method.<br>
<strong>Results:</strong> There were no significant differences in the frequencies of genotype and allele frequency in ESR-β gene rs4986938 polymorphism between patients and control groups (p>.005). In contrast, we observed a significant difference in the frequencies alleles and genotypes of rs9340799 and rs2234693 (ESR-α) and rs1256049 (ESR-β) polymorphisms between patients and control groups.<br>
<strong>Conclusion: </strong>We demonstrated that rs9340799 and rs2234693 (ESR-α), and rs1256049 (ESR-β) polymorphisms may play important role in implantation failure in women in northwest of Iran. However, more studies on different geographic areas, races and ethnicities are required to determine exact role of ESR-α and ESR-β genes polymorphisms in implantation failure.</div>
Polymorphisms, ESR-α, ESR-β, Implantation failure
12
19
http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-452-3&slc_lang=en&sid=1
Parisa
Pagard
parisapagard@gmail.com
10031947532846004064
10031947532846004064
No
Department of Genetic, Tabriz Branch, Islamic Azad University, Tabriz, Iran
Seyed Ali
Rahmani
seyyedalirahmani@gmail.com
10031947532846004065
10031947532846004065
Yes
Department of Medical Genetics, Tabriz University of Medical Sciences, Tabriz, Iran
Lida
Heidari
lida.heidari@yahoo.com
10031947532846004066
10031947532846004066
No
Gynecology Department, Eastern Azerbaijan ACECR ART Center, Eastern Azerbaijan Branch of ACECR, Tabriz, Iran