Journal of Basic Research in Medical Sciences
مجله ی تحقیقات پایه در علوم پزشکی
Journal of Basic Research in Medical Sciences
Medical Sciences
http://jbrms.medilam.ac.ir
1
admin
2383-0506
2383-0972
doi
en
jalali
1399
6
1
gregorian
2020
9
1
7
4
online
1
fulltext
en
High prevalence of Toxoplasma gondii infection in ovine aborted fetuses in Gilan Province, Iran: Molecular detection and genotype characterization
Parasitologiy
Parasitologiy
پژوهشي
Research
<div><strong>Introduction:</strong> Sheep farming is one of the most important economic aspects of the livestock industry in Gilan province, Iran. Toxoplasmosis is a prevalent zoonotic disease and a major cause of abortion, congenital infection, and stillbirth in animals and humans. Previously, it was assumed that sheep are mostly infected with <em>Toxoplasma gondii</em> (<em>T. gondii</em>) after birth. Nevertheless, recent studies have shown that <em>T. gondii </em>congenital transmission is more prevalent than previously speculated. Therefore, determining the genotypes of this parasite in the intermediate host plays an important role in human infections and prevention programs. This cross-sectional study aimed to determine the <em>T. gondii</em> genetic diversity in aborted ovine fetuses during the lambing season (2018-2019) in Gilan province, Iran.<br>
<strong>Materials and Methods:</strong> Molecular detection of <em>T. gondii </em>was performed in 44 brain tissue samples, collected from aborted ovine fetuses, using a nested polymerase chain reaction (nested-PCR) assay, to target the <em>GRA6</em> gene. Also, the nested-PCR products belonging to the <em>GRA6</em>-positive samples underwent genotyping with the help of <em>Tru1I </em>(<em>MseI</em>) restriction enzyme using the PCR-restriction fragment length polymorphism (PCR-RFLP) method to classify strains into one of the three major lineages of <em>T. gondii</em><em>.</em><br>
<strong>Results:</strong> The results revealed infection in 30 (68.18%) out of 44 brain samples of aborted ovine fetuses, based on the nested-PCR assay with <em>GRA6 </em>gene<em>. </em>Also, the PCR-RFLP results demonstrated the predominance of type II <em>T. gondii</em> in all of the isolates.<br>
<strong>Conclusion: </strong>Overall, the present results revealed the high incidence of <em>T. gondii</em> infection through congenital transmission.This is the first molecular detection and genotyping of <em>T. gondii</em> in ovine aborted fetuses in Gilan Province, Iran.</div>
Toxoplasmosis, Sheep, Abortion, Genotyping, Iran
53
62
http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-518-1&slc_lang=en&sid=1
Mohammad Reza
Chaechi Nosrati
mohammadchaichi1@gmail.com
10031947532846004143
10031947532846004143
No
Department of Pathobiology, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran
Bahar
Shemshadi
bshemshadi@yahoo.com
10031947532846004144
10031947532846004144
Yes
Department of Pathobiology, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran
Parviz
Shayan
pshayan@ut.ac.ir
10031947532846004145
10031947532846004145
No
Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
Shahrokh
Ranjbar Bahadori
bahadory_2000@yahoo.com
10031947532846004146
10031947532846004146
No
Department of Parasitology, Faculty of Veterinary Medicine, Garmsar Branch, Islamic Azad University,Gamsar, Iran
Ali
Eslami
alieslami1936@gmail.com
10031947532846004147
10031947532846004147
No
Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran