Journal of Basic Research in Medical Sciences
مجله ی تحقیقات پایه در علوم پزشکی
Journal of Basic Research in Medical Sciences
Medical Sciences
http://jbrms.medilam.ac.ir
1
admin
2383-0506
2383-0972
doi
en
jalali
1393
9
1
gregorian
2014
12
1
1
4
online
1
fulltext
en
Induction of type 2 diabetes with high concentration and long term fructose intake in male Sprague-Dawley rats
Physiology
Physiology
پژوهشي
Research
Introduction: High calorie diet is becoming the most important health problem in most developed western societies. Diets with high caloric contents such as high fructose intake are associated with increasing risk of type two diabetes (T2D) and insulin resistance (IR). However, large differences have been seen in the dose and duration of fructose consumption to induction of T2D. Interest to develop animal models, in which IR is induced by feeding a high caloric diet, is the main aim of this study. Materials and methods: Male Sprague-Dawley rats were randomly sorted into three groups, control and fructose-treated (10 and 20 %) animals. After 6 and 14 weeks, fluid intake, body weight, intraperitoneal glucose tolerance test, metabolic parameters (glucose, insulin, triglyceride, cholesterol, uric acid) and homeostasis model assessment of insulin resistance were checked. Results: There was no difference between the body weight of the fructose-treated (10 and 20 %) and control groups during the experiment. Fluid intake in fructose-treated ones (10 and 20 %) was significantly higher than the control group from third week to the end of experiment. Intraperitoneal glucose tolerance test was the same for all groups in either sixth or fourteenth weeks. Fasting plasma insulin, triglyceride, uric acid and homeostasis model assessment of insulin resistance and glucose tests showed significant enhancement in fructose-treated rats (20%) only after 14 weeks. Conclusion: Consumption of high fructose solution (20%) for 14 weeks could induce IR, but using of low dose of fructose (10% for 6 or 14 weeks) didn’t develop it.
Fructose, Insulin resistance, Type two diabetes, Sprague-Dawley rat
42
50
http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-99-1&slc_lang=en&sid=1
Enaiat
Anvari
Anvari_ph@yahoo.com
10031947532846003552
10031947532846003552
No
Department of physiology, Shiraz University of Medical Sciences, Shiraz, Iran
Sara
Keshtgar
Anvari_ph@yahoo.com
10031947532846003553
10031947532846003553
No
Ali
Noorafshan
Anvari_ph@yahoo.com
10031947532846003554
10031947532846003554
No
Ali
Rafati
A_rafati@yahoo.com
10031947532846003555
10031947532846003555
Yes