en Medical genetics Medical genetics پژوهشي Research <span style="font-size:9pt"><span minion="" pro="" style="font-family:"><b>Introduction</b>: Deafness is one of the most common sensory-neural disorders, with 80% of hereditary deafness being non-syndromic. The aim of this study was to investigate the frequency of the 167delT mutation of the <i>gap junction protein beta 2</i> (<i>GJB2</i>) gene in the deaf population of non-syndromic West Iran.</span></span><br> <span style="font-size:9pt"><span minion="" pro="" style="font-family:"><b>Materials & Methods</b><b><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">: </span></span></b>Seventy individuals were included in the study, with 34 being deaf and 36 being healthy. The RFLP-PCR technique was used to detect the 167delT mutation.<span lang="FA" dir="RTL" style="font-family:"Times New Roman","serif""></span></span></span><br> <span style="font-size:9pt"><span minion="" pro="" style="font-family:"><b>Results</b><b><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">: &nbsp;</span></span></b>The frequency of the homozygous mutant 167delT was 5.7% (4 individuals, equivalent to 8 chromosomes), with the heterozygous carriers at 75.7% (53 individuals, equivalent to 106 chromosomes), and the homozygous genotype in healthy individuals was 18.6% (13 individuals, equivalent to 26 chromosomes). In deaf individuals, the frequency of the homozygous mutant 167delT was 5.7% (4 individuals, equivalent to 8 chromosomes), the heterozygous genotype was 39.6% (27 individuals, equivalent to 54 chromosomes), and the frequency of the wild-type homozygous genotype was 4.7% (3 individuals, equivalent to 6 chromosomes). In healthy individuals, there was zero mutant genotype, 36.1% heterozygous carriers (26 individuals, equivalent to 52 chromosomes), and 13.9% wild-type homozygous (10 individuals, equivalent to 20 chromosomes).</span></span><br> <span style="font-size:9pt"><span minion="" pro="" style="font-family:"><b>Conclusion</b><b><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">: &nbsp;</span></span></b>Gene mutations play a role in causing non-syndromic autosomal recessive deafness in our statistical population from Kermanshah Province. The <i>GJB2</i> 167delT mutant is responsible for 5.7% of ARNSHL deafness in the population of Kermanshah, which differs from the rates reported in other parts of Iran.</span></span><br> &nbsp; non-syndromic deafness, 167delT, GJB2 gene, ARNSHL deafness 19 30 http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-168-9&slc_lang=en&sid=1 Parivash Bakhshipour Parivash.bakhshipour@gmail.com 10031947532846008308 0009-0007-3214-8973 No Department of Biology, Sa.C., Islamic Azad University, Sanandaj, Iran Marzieh Karami Marzieh.Karami@iau.ac.ir 10031947532846008309 10031947532846008309 No Department of Nursing and Midwifery, Sa.C., Islamic Azad University, Sanandaj, Iran Fatemeh Keshavarzi gol.keshavarzi@gmail.com 10031947532846008310 10031947532846008310 Yes Department of Biology, Sa.C., Islamic Azad University, Sanandaj, Iran