en Clinical microbiology Clinical microbiology پژوهشي Research <span style="font-size:9pt"><span minion="" pro="" style="font-family:"><b>Introduction</b>: This study aimed to measure the amount of estrogen and heavy metals (cadmium, mercury, lead, and arsenic) in the liver of industrial chickens from the selected areas of Ilam City, Iran.</span></span><br> <span style="font-size:9pt"><span minion="" pro="" style="font-family:"><b>Materials & Methods</b><b><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">: </span></span></b>This study is a cross-sectional study conducted on 30 liver tissue samples randomly selected from poultry farms in Ilam, Iran. The concentrations of the heavy metals (mg/kg) and 17-beta estradiol (ppb) were measured using atomic absorption spectrometer (AAS) and high-performance liquid chromatography (HPLC), respectively. The SPSS software version 21 was used for the data analysis.<span lang="FA" dir="RTL" style="font-family:"Times New Roman","serif""></span></span></span><br> <span style="font-size:9pt"><span minion="" pro="" style="font-family:"><b>Results</b><b><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">: &nbsp;</span></span></b>The mean level of 17-beta estradiol was 3.578 &plusmn; 0.756 ppb compared to the standard limit of 0.24 ppb (p < 0.05). The mean level of arsenic was 0.942 &plusmn; 0.842 mg/kg versus the standard limit of 0.1 mg/kg versus the standard limit of 0.24 ppb (p < 0.05). The mean concentrations of lead, mercury, and cadmium were 0.03&plusmn;0.03, 0.11&plusmn;0.13, and 0.015&plusmn;0.02 mg/kg, respectively, and the differences were not significant compared to the standard values (all p values >0.05). The results showed no correlation between mean heavy metal levels and 17-beta estradiol. The target hazard quotient for arsenic was calculated to be 1.33.</span></span><br> <span style="font-size:9pt"><span minion="" pro="" style="font-family:"><b>Conclusion</b><b><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">: &nbsp;</span></span></b>The results show that the average levels of 17-beta estradiol and arsenic in the samples were higher than the standard limit. This may threaten the general health of residents in the area.</span></span><br> &nbsp; Chicken, Estrogen, Heavy metal, Lead, Mercury, Cadmium, Arsenic 31 39 http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-915-8&slc_lang=en&sid=1 Kosar mennati kosarmennati1996@gmail.com 10031947532846008311 0009-0008-0392-7887 No School of Medicine, Ilam University of Medical Sciences, Ilam, Iran Naser Abbasi ilamfarma@gmail.com 10031947532846008312 10031947532846008312 No Biotechnology and Medicinal Plants Research Center, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran Zahra Vahdat Shariatpanahi z.shariatpanahi@sbmu.ac.ir 10031947532846008313 10031947532846008313 No Department of Clinical Nutrition and Dietetics, Faculty of Nutrition and Food Technology, National Nutrition and Food Technology Research Institute, Shahid Beheshti University of Medical Sciences, Tehran, Iran Shaahin Shahbazi mdkabe@gmail.com 10031947532846008314 10031947532846008314 Yes School of Medicine, Ilam University of Medical Sciences, Ilam, Iran