en پژوهشي Research <div><strong>Introduction: </strong>Diabetes is one of the leading causes of mortality in the world. The present study aimed to investigate the effect of aerobic <a name="_Hlk46211722">exercise</a> training and a synthesized insulin nanocomposite hydrogel on TNF-&alpha; and IL-6 in type 1 diabetic rats.<span dir="RTL"></span><br> <strong>Materials and methods: </strong>Twenty-five rats were divided into five equal groups of animals each containing five animals, control - healthy, Control-diabetes, Nano-insulin Diabetes, Exercise Diabetes and Nano-insulin Diabetes-Exercise. The exercise training program lasted eight weeks. After the five days of familiarization, exercise time for the exercise groups were as follows: 20 m/min for the first and second weeks, 25 m/min for the third and fourth weeks and 30 m / min for the fifth and sixth weeks. The rats were also given the Nano-insulin supplement. Rats were killed 48 hours after the last training session. Their plasma was taken and used for the analysis of markers. <strong><span dir="RTL"></span></strong><br> <strong>Results: </strong>There were significant differences in IL-6 (P<0.001) and TNF-&alpha; (P<0.001) between the groups.<br> <strong>Conclusion: </strong>The results of this study showed that aerobic exercise training along with Nano-insulin supplementation significantly reduced TNF-&alpha; and IL-6 in rats with type 1 diabetes and these changes were more in the Nano-insulin Diabetes group.</div> Aerobic training, Synthesized insulin nanocomposite hydrogel, TNF-α, IL-6, Type 1 diabetic rat 38 46 http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-361-3&slc_lang=en&sid=1 Seyed nabiyollah Hoseini nabi562017@gmail.com 10031947532846003856 10031947532846003856 No Department of Exercise Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran Asieh Abbassi Daloii abbasi.daloii@gmail.com 10031947532846003857 10031947532846003857 Yes Department of Exercise Physiology, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran Seyed Javad Ziaolhagh javadzia@gmail.com 10031947532846003858 10031947532846003858 No Department of Exercise Physiology, Shahroud Branch, Islamic Azad University, Shahroud, Iran Ayoub Saeidi saeidi_as68@yahoo.com 10031947532846003859 10031947532846003859 No Department of Physical Education, Damghan Branch, Islamic Azad University, Damghan, Iran