en Physical medicine Physical medicine پژوهشي Research <div><strong>Introduction: </strong>Alzheimer&#39;s disease (AD) is a disease associated with disorders of the nervous system. Inflammation in the central nervous system can play a major role in the formation of AD<span dir="RTL">.</span> This study aimed to investigate the effect of AD induction as well as running on positive and negative slopes on TNF-&alpha; and INF-&gamma; gene expression in the muscle tissue of rats with AD.<br> <strong>Materials and methods: </strong>In this experimental study 15 rats were injected with 8 mg/kg Trimethyltin chloride (TMT) intra- peritoneally. After being assured of AD rats were divided in three groups of 5 rats, including: 1) control, 2) running in positive slope, and 3) running in negative slope. To investigate the effects of AD induction on the TNF-&alpha; and INF-&gamma; gene expression levels, 5 rats were assigned to the healthy control group. During eight weeks the positive running group (at a speed of 16 m/min in positive upward slope) and negative running group (at a speed of 16 m/min in negative downhill slope) ran on the treadmill for five sessions per week and 60 minutes per session. The Shapiro- Wilk test was used for investigate the normal distribution of data and one-way ANOVA with LSD <em>post- hoc</em> tests were used to analyze the data (P<0.05).<br> <strong>Results: </strong>AD induction by TMT significantly increased TNF-&alpha; (P=0.004) and INF-&gamma; (P=0.04), nevertheless running on positive and negative slope significantly decreased TNF-&alpha; (P=0.001) also running on negative slope significantly decreased INF-&gamma; (P=0.04)<span dir="RTL">.</span></div> <strong>Conclusion: </strong>Running on a positive and negative slope seems to improve TNF-&alpha; gene expression in rats with AD. Running, Alzheimer's disease, TNF-α, INF- γ 35 42 http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-464-1&slc_lang=en&sid=1 Mehdi Noura noora.mehdi@yahoo.com 10031947532846003646 10031947532846003646 No Department of Sport Physiology, South Tehran Branch, Islamic Azad University, Tehran, Iran Sajjad Arshadi arshadi.sajjad@yahoo.com 10031947532846003647 10031947532846003647 Yes Department of Sport Physiology, South Tehran Branch, Islamic Azad University, Tehran, Iran Ardeshir Zafari ardeshirzafari@gmail.com 10031947532846003648 10031947532846003648 No Department of Sport Physiology, Zanjan Branch, Islamic Azad University, Zanjan, Iran Abdolali Banaeifar alibanaeyfar@yahoo.com 10031947532846003649 10031947532846003649 No Department of Sport Physiology, South Tehran Branch, Islamic Azad University, Tehran, Iran