en پژوهشي Research <div><strong>Introduction:&nbsp;</strong>One of the famous cancer treatments that are associated with an increment in inflammatory factors is X-ray radiation. This study aimed to evaluate the effect of high-intensity interval training on inflammatory markers in male rats undergoing X-ray radiation.<br> <strong>Materials and Methods:</strong>&nbsp;In this experimental study, 24 male rats were randomly divided into 4 groups: healthy control (n=6), HIIT (n=6), X-ray radiation (n=6) and X-ray radiation + HIIT (n=6). first, X-ray radiation and HIIT + X-ray radiation groups were anesthetized with ketamine xylazine solution (K, 60-90 kg/mg; Z, 6-10 kg/mg) and then placed on a 1 cm thick Plexiglas plate. The X-ray radiation photon beam was performed using X-ray with 4 Gy linear accelerators (Elekta Compact 6-MV China). The training program included ten weeks of HIIT. Serum levels of Tumor Necrosis Factor Alpha (TNF-&alpha;) and <a name="_Hlk86773301">Interleukin-6 (IL-6) </a>were measured by rat ELISA Kits. One-way ANOVA was used to determine the existence of a significant difference among groups.<br> <strong>Results:</strong>&nbsp;The results showed a significant difference between TNF-&alpha; and IL-6 in the HIIT, X-ray radiation, and HIIT + X-ray radiation groups with the control group (P < 0.05), but there was no significant difference between these three groups (P > 0.05).<br> <strong>Conclusion:</strong>&nbsp;X-ray radiation can increase TNF-&alpha; and IL-6. HIIT, on the other hand, can also enhance these factors. It seems better for people undergoing radiotherapy to use another exercise training instead of HIIT.&nbsp;</div> X-ray radiation, High-intensity interval training, TNF-α, IL-6 10 18 http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-512-4&slc_lang=en&sid=1 Mohammad Parastesh M-parastesh@araku.ac.ir 10031947532846007671 10031947532846007671 No Department of Sports Physiology, Faculty of Sport Sciences, Arak University, Arak, Iran Mohammadreza Bayatiani bayatiani@arakmu.ac.ir 10031947532846007672 10031947532846007672 No Department of Radiotherapy and Medical Physics, Faculty of Para Medicine, Arak University of Medical Sciences and Khansari Hospital, Arak, Iran Mina Khaleghi kebria141@gmail.com 10031947532846007673 10031947532846007673 No Department of Sports Physiology, Faculty of Sport Sciences, Arak University, Arak, Iran Mohammadreza Sadeghian Shahi rsadeghian@yazd.ac.ir 10031947532846007674 10031947532846007674 Yes Department of Physical Education and Sports Science, School of Psychology and Educational Sciences, Yazd University, Yazd, Iran Farahnaz Ayatizadeh Tafti 10031947532846007675 10031947532846007675 No Department of Physical Education and Sports Science, School of Psychology and Educational Sciences, Yazd University, Yazd, Iran Behzad Aria b.aria@yazd.ac.ir 10031947532846007676 10031947532846007676 No Department of Physical Education and Sports Science, School of Psychology and Educational Sciences, Yazd University, Yazd, Iran