en پژوهشي Research <div style="text-align: justify;"><span style="font-size:9pt"><span minion="" pro="" style="font-family:"><b>Introduction</b>: A study was undertaken to investigate the presence of house dust mites in Ilam town and their climate-induced variability between January and June 2017.</span></span><br> <span style="font-size:9pt"><span minion="" pro="" style="font-family:"><b>Materials & Methods</b>: In this cross-sectional investigation, a total of fifty houses located in five different residential areas of Ilam town were selected using a cluster random sampling method. Dust samples, collected from mattresses, pillows, and carpets, were obtained from each house utilizing a portable vacuum cleaner. Each sample was then transferred into 70% alcohol solution, with an amount equivalent to 200mg preserved for further analysis. Under a stereomicroscope, mites were carefully isolated from the samples using micro-dissecting needles. Subsequently, these specimens underwent clearing processes before being mounted on Hoyer media for preservation purposes. The identification process involved employing standard taxonomic keys to determine the species of each specimen. Furthermore, statistical analyses provided both mean values as well as confidence intervals (CI) for the mite populations collected for every identified species. Additionally, binomial tests served to assess the distribution patterns across these species.</span></span><br> <span style="font-size:9pt"><span minion="" pro="" style="font-family:"><b>Findings</b>: Out of a grand total of 208 mite specimens analyzed during this investigation phase, seven distinct species namely <i>Haemolaelaps glasgowi</i> (24.52%), <i>Echinolaelaps echidninus</i> (12.02%), <i>Laelaps nuttalli</i> (11.06%), <i>Androlaelaps setosus</i> (13.94%), <i>Neoeucheyla iranica</i> (10.10%), <i>Cheyletus malaccensis</i> (14.90%), and <i>Ornithonyssus bacoti</i> (13.46%) were successfully identified. The distribution of all species displayed a significant statistical value (P < 0.01). Furthermore, an astonishing 80% of the visited households were infested with mites.</span></span><br> <span style="font-size:9pt"><span minion="" pro="" style="font-family:"><b>Conclusion</b><b><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">: </span></span></b>This particular study has shed light on the presence of a remarkably diverse array of house dust mite species. The prevalence of certain rodent-related mites suggests that rodents either inhabit or frequent residential homes, prompting the need for further investigation.</span></span></div> House dust mites, Fauna, Allergens, Iran, Ilam 12 20 http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-54-3&slc_lang=en&sid=1 Narges Sharifinia narges.sharifinia@gmail.com 10031947532846007522 10031947532846007522 No Department of Vector Biology and Control, School of Health, Ilam University of Medical Sciences, Ilam, Iran Fariba Mokhtari entomologistf@yahoo.com 10031947532846007523 10031947532846007523 No Department of Vector Biology and Control, School of Health, Ilam University of Medical Sciences, Ilam, Iran Aboozar Soltani abu2sol@yahoo.com 10031947532846007524 10031947532846007524 No Research Centre for Health Sciences, Department of Vector Biology and Control, School of Health, Shiraz University of Medical Sciences, Shiraz, Iran Manijeh Hoseiny-Rad m.hosseinirad85@gmail.com 10031947532846007525 10031947532846007525 No Department of Biology, Farhangian University, Tehran, Iran Ali-Ashraf Aivazi ali.medilam@gmail.com 10031947532846007526 10031947532846007526 Yes Department of Vector Biology and Control, School of Health, Ilam University of Medical Sciences, Ilam, Iran