:: Volume 5, Issue 2 (3-2018) ::
2018, 5(2): 8-13 Back to browse issues page
Time dependent of epigenetic effect of disulfiram on tumor suppressor gene of RASSF1A in Hela cancer cell line
Seyedeh naghme Noorirad , Mohsen Pourghasem , Farideh Feizi , Zeinab Abedian , Masoumeh Ghasemi , Zahra Babazadeh * , Navid Rabiee
Department of Anatomical Sciences, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran , zbabazadeh@gmail.com
Abstract:   (4461 Views)
Introduction: Cervical cancer is the third most common tumor among women. Surgery, radiotherapy, and chemotherapy are common treatments, however high stage tumors have frequently poor prognosis. Nowadays, the epigenetic reversion introduced as an efficient strategy of treatment of cervical cancer. In the process, inhibitors of DNA methyltransferase (DNMT) induce re-expression of tumor suppressor genes. Among these inhibitors, disulfiram (DSF) has been suggested as non-nucleoside analogous. In this research, we evaluated the epigenetic effect of DSF on demethylation of the tumor suppressor gene, RASSF1A, in Hela cell line.
Materials and methods: Hela cells were cultured and treated with different doses from 2.5 to 37.5µM during 24, 48 and 72 hours. MTT assay was carried out to find half maximal inhibitory concentration (IC50). The methylation specific PCR (MSP) assay was applied to evaluate methylation pattern.
Results: The IC50 of DSF was determined at the 2.5, 12.5, and 15µM after72 hours. The MSP results showed partial demethylation at mentioned concentrations after 72h but unmethylated band was not observed after 24h.
Conclusion: Our findings indicated that, IC50 of DSF exerted a biphasic effect in Hela cell line and at least 72 hours treatment is needed for the epigenetic reversion of DSF on RASSF1Ain Hela cell line.
Keywords: Disulfiram, RASSF1A, Epigenetic reversion, Methylation, Cervical cancer
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Type of Study: Research | Subject: Genetics
Received: 2017/06/1 | Accepted: 2017/08/4 | Published: 2018/01/26

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