A Comparative Analysis of Two High-Intensity Interval Training (HIIT) Programs on PGC-1α, p53, and Citrate Synthase Protein Levels in Cardiomyocytes of Male Type 2 Diabetic Rats

Khayampour , Nadia; Peeri, Maghsoud; Azarbayjani , Mohammad Ali

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Volume 10, Issue 4 (12-2023)

2023, 10(4): 54-66

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A Comparative Analysis of Two High-Intensity Interval Training (HIIT) Programs on PGC-1α, p53, and Citrate Synthase Protein Levels in Cardiomyocytes of Male Type 2 Diabetic Rats

Nadia Khayampour

, Maghsoud Peeri

, Mohammad Ali Azarbayjani

Department of Exercise Physiology, Physical Education Faculty, Central Tehran Branch, Islamic Azad University, Tehran, Iran , m.peeri@iauctb.ac.ir

Abstract: (660 Views)

Introduction: This study investigates the impact of two high-intensity interval training (HIIT) programs on PGC-1α, p53, and citrate synthase (CS) proteins within cardiomyocytes of male type 2 diabetic rats, aiming to discern potential molecular mechanisms influencing cardiac health.
Material & Methods: Twenty-four male Wistar rats were randomly assigned to control (NC), diabetic control (DC), diabetic with type 1 HIIT (HIIT-1), and diabetic with type 2 HIIT (HIIT-2) groups. Streptozotocin (STZ) induced type 2 diabetes, excluding the NC group. A four-week HIIT intervention, six sessions per week, preceded the analysis of heart tissue for PGC-1α, p53, and CS protein levels. Statistical analysis employed GraphPad Prism software version 8 and one-way ANOVA (P < 0.05).
Results: Both HIIT-1 (p=0.004) and HIIT-2 (p=0.007) groups exhibited significantly elevated cardiac PGC-1α levels compared to DC. CS levels increased notably in HIIT-1 (p=0.001) and HIIT-2 (p<0.001), with HIIT-2 surpassing HIIT-1 significantly (p=0.010). Concurrently, p53 levels significantly decreased in both HIIT-1 (p=0.005) and HIIT-2 (p=0.001) groups compared to DC.
Conclusion: Exercise training (HIIT) positively influences cardiac metabolism, evident in PGC-1α and CS upregulation and p53 downregulation. While these findings provide valuable insights, further exploration is crucial for a comprehensive understanding of the underlying molecular mechanisms. This study advances our understanding of optimizing exercise interventions for improved cardiac health in type 2 diabetes.

Keywords: Exercise Therapy, Type 2 Diabetes Mellitus, Mitochondrial Diseases, PGC-1alpha, Tumor Suppressor Protein p53, Citrate (si)-Synthase

Full-Text [PDF 1268 kb] (146 Downloads)

Type of Study: Research | Subject: Physiology
Received: 2022/02/23 | Accepted: 2022/02/26 | Published: 2023/12/28

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Khayampour N, Peeri M, Azarbayjani M A. A Comparative Analysis of Two High-Intensity Interval Training (HIIT) Programs on PGC-1α, p53, and Citrate Synthase Protein Levels in Cardiomyocytes of Male Type 2 Diabetic Rats. Journal of Basic Research in Medical Sciences 2023; 10 (4) :54-66
URL: http://jbrms.medilam.ac.ir/article-1-671-en.html

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