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Gholamreza Jahangirzadeh Sooreh, Seyed Babak Khalifzadeh Kalibar,
Volume 9, Issue 3 (6-2022)
Abstract
Introduction: SEPT12 gene encode a testis-specific protein that play important role in terminal differentiation of germ cells. The product of this gene involved in sperm tail annulus constituent, and is essential for head-tail formation in sperm. Various polymorphisms on SEPT12 gene are identified that are associated with impairment of sperm function as well as spermatogenesis in males with infertility. In this study we investigated correlation of rs6500633 polymorphism in the SEPT12 gene in Iranian Azeri male with idiopathic asthenozoospermia (AZS).
Materials and Methods: We enrolled 50 men with idiopathic AZS as case group and 50 healthy men as control group from East Azerbaijan, Iran. Extraction of genomic DNA was conducted by proteinase K method from sperm samples. Genotyping was performed by tetra-primer amplification refractory mutation system-polymerase chain reaction (Tetra-ARMS PCR).
Results: Frequency of TT, TC, and CC genotypes were 10%, 86%, and 4% in the AZS. On the other hand, the frequencies were 4%, 90%, and 6% in the healthy controls, respectively. Our study indicated no significant difference between the patient and control groups for frequency of the rs6500633 polymorphism in the SEPT12 gene (P > 0.05).
Conclusion: We demonstrated no significant association between rs6500633 polymorphism in the SEPT12 gene and AZS among Iranian Azeri men.
Hamed Mohammadzadeh Zangalani, Seyed Babak Khalilzadeh Kaleybar,
Volume 10, Issue 1 (1-2023)
Abstract
Introduction: Asthenozoospermia (AZS), sperm immobility, is one of the major cause of men infertility. Evidence suggested that microRNAs (miRNAs) play a critical role in spermatogenesis process. However, the association of miRNAs polymorphisms with idiopathic male infertility remains unknown. Therefore, we investigated correlation between miR-196a2 rs11614913 polymorphism and idiopathic AZS among Iranian Azeri men.
Materials and Methods: In this study, 50 men with idiopathic AZS (case group) as well as 50 age and ethnically matched healthy men (control group) were enrolled from East Azerbaijan, Iran. The proteinase K method was used to extract the genomic DNA from sperm samples. Finally, genotyping was conducted using tetra-primer amplification refractory mutation system-polymerase chain reaction (Tetra-ARMS PCR) method.
Results: The frequency of TT, TC, and CC genotypes were 12%, 54%, and 34%, respectively, in patients with AZS; whereas the figures were 8%, 40%, and 52% in healthy controls, respectively. We found a significant difference between case and control groups in term of CC genotype frequency (P = 0.016).
Conclusion: We found a significant correlation between miR-196a2 rs11614913 polymorphism and AZS in Iranian Azeri men.
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