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Showing 6 results for Oodi
Roohollah Mirzaee Khalilabadi, Fatemeh Yari, Naser Amirizadeh, Mahin Nikougoftar, Arezoo Oodi,
Volume 4, Issue 2 (3-2017)
Abstract
Introduction: Recognition of human leukocyte antigens (HLA) is of importance for hematopoietic stem cell transplantation. Any HLA-mismatches between the donor and recipient can cause graft rejection or other complications. In HLA-typing experiments, usage of HLA-known reference cells accompany with HLA-unknown samples is obligatory. Some international centers represent these cells with high expenses. On the other hand, transferring of these cells is problematic and in some instances is not practical. In this study, we introduced umbilical cord-derived mesenchymal stem cells (MSCs) as reference cells for HLA genotyping. These cells are national and can be prepared locally.
Materials and methods: We isolated MSCs from three umbilical cord and after their growth and proliferation, these cells were characterized by flow cytometry technique using antibodies to CD29, CD34, CD44, CD45, CD73, CD90 and CD105. HLA-typing was then carried out by PCR-SSP kits for HLA-A, -B and -DRB allele’s identification.
Results: Isolated MSCs were positive for MSCs markers; CD29, CD44, CD73, CD90, and CD105 and negative for hematopoietic stem cell markers; CD34 and CD45. HLA alleles were determined. One of the samples was homologous for HLA alleles and the others were heterologous.
Conclusion: We can develop a reference panel for HLA-typing by obtaining MSCs from available sources like umbilical cord.
Aazam Khani Daramroodi, Fatemeh Keshavarzi, Farshid Raissi,
Volume 5, Issue 2 (3-2018)
Abstract
Introduction: Group B Streptococcus (GBS) is one of the most causes of neonatal infections. The bacterium colonizes genitourinary tracts of pregnant women and transmits to infants. The aim of this study was investigating colony PCR and culture methods to detection of GBS in pregnant women.
Materials and methods: Hundred pregnant women, at the 35th and 37th weeks of pregnancy, were selected from the Obstetrics and Gynecology Unit of the Moatazedi and Shahid Chamran Hospitals in Kermanshah province. Specimens were collected from vaginal introitus and investigated by selective culture and colony PCR methods. Then, antibiotic resistance tests were performed according to the latest guidelines of Clinical and Laboratory Standards Institute (CLSI).
Results: Prevalence of GBS colonization was shown to be 5% and 6% by the culture and colony PCR methods, respectively. Also, resistance rate to erythromycin, penicillin, vancomycin, and the clindamycin were determined to be 50%, 16.66%, 16.66% and 33.33 %, respectively. Moreover, the highest resistance was for erythromycin and the appropriate antibiotics were penicillin and then vancomycin.
Conclusion: A higher prevalence of GBS colonization in pregnant women in the Kermanshah city of Iran was detected using colony PCR method compared to culture method.
Gelavij Mahmoodi, Sabrieh Amini,
Volume 6, Issue 1 (1-2019)
Abstract
Introduction: The role of medicinal plants in enhancement of memory and improvement of Alzheimer disease symptoms has attracted researchers’ attention. Genus Salvia (sage) is the largest and most valuable type of herbal medicine from Lamiaceae family, and its therapeutic effects have long been considered. This study investigated the effect of hydroalcoholic extract of Salvia officinalis (S. officinalis) leaves on scopolamine-induced amnesia in adult male mice.
Materials and methods: A step-through inhibitory avoidance task was used for memory assessment. Animals received hydroalcoholic extract of Salvia (10, 20 or 40 mg/kg) 30 minutes after administration of scopolamine (1 mg/kg, intraperitoneally) after training and before testing, (based on experimental design). Animals were tested 24 h after the training session, and step-through latency in entering the dark compartment was recorded as passive avoidance memory. The data were analyzed by one-way ANOVA, followed by Tukey test. Statistical significance level was set at P<0.05.
Results: The results indicated that administration of scopolamine impaired both consolidation and retrieval of passive avoidance memory. Administration of 40 mg/kg hydroalcoholic extract of Salvia after training, or 20 and 40 mg/kg on the day of experiment ameliorated the effect of scopolamine.
Conclusion: The hydroalcoholic extract of Salvia can inhibit scopolamine-induced impairment of passive avoidance memory in mice.
Fatemeh Keshavarzi, Shahnaz Chaghakaboodi, Abdolrasool Khalafi,
Volume 6, Issue 2 (3-2019)
Abstract
Introduction: Epithelial–mesenchymal transition (EMT) phenomenon in cancer cells is one of the most sensitive stages in metastasis. In addition, it has been shown that many molecular factors and cellular signals, including the Sox2 gene, are involved in arising EMT, which together cause EMT. The phenomenon of EMT in cancer cells is one of the most sensitive stages in metastasis. Expression of Sox2 gene as an effective gene in altering aggressive behavior of cancer cells has not been studied in patients with gastric cancer.
Materials and methods: RNA was extracted from 50 tumoral and 50 normal samples from patients with gastric cancer. Then cDNA synthesis was performed on the extracted RNAs. Primers for the target genes were designed and synthesized. Quantitative real-time PCR was performed to determine the relative expression of the studied genes and the relationship between the clinic pathologic parameters and the amount of RNA was analyzed by Pearson correlation coefficient analysis.
Results: mRNA expression of Sox2 in tumor tissue was significantly higher than that in healthy adjacent tumor tissue. The mRNA expression level of Sox2 in tumor tissue was significantly higher than that in healthy adjacent tumor tissue. Also, mRNA levels of Sox2 in tumor tissue indicated a direct and significant correlation with tumor staging (TNM stages) (τ = 0.329, P = 0.02). In addition, there was no significant correlation between mRNA levels of Sox2 in tumor tissue and tumor size (τ = 0.138, P = 0.177).
Conclusion: Assessment of Sox2 gene expression in the study of genes involved in EMT process in gastric cancer patients and its relation to pathologic, clinical and metastatic findings in gastric cancer is an effective method in the diagnosis of gastric cancer.
Mohammad Mianroodi, Siham Touchal,
Volume 6, Issue 3 (6-2019)
Abstract
Joint replacement surgery in the wrist is less common than other replacement, but can be an option if you have painful arthritis that does not respond to other treatments.
In wrist joint replacement surgery, the damaged parts of the wrist bones are removed and replaced with artificial components, called a wrist prosthesis. If the cartilage is worn away or damaged by injury, infection, or disease, the bones themselves will rub against each other, wearing out the ends of the bones. This causes a painful, arthritic condition. Osteoarthritis, the most common form of arthritis, results from a gradual wearing away of the cartilage covering on bones. Rheumatoid arthritis is a chronic inflammatory disease of the joints that results in pain, stiffness and swelling. Rheumatoid arthritis usually affects several joints on both the right and left sides of the body. Both forms of arthritis may affect the strength of the fingers and hand, making it difficult to grip or pinch.
Fatemeh Poodineh , Abbas Salehikia , Dr Maryam Banparvari , Zahra Raghi,
Volume 11, Issue 1 (1-2024)
Abstract
Introduction: Osteoporosis, characterized by diminished bone mass and increased fracture susceptibility, prompts the investigation into the impact of a four-month TRX training regimen on femur neck bone mineral density (BMD) and its correlation with serum leptin levels in women with osteopenia.
Material & Methods: In this quasi-experimental study, 30 women with osteopenia were purposefully selected and randomly assigned to TRX training (n = 15) or control (n = 15) groups. The intervention group underwent TRX exercises thrice weekly for 45-60 minutes per session for four months. Blood samples collected at baseline and post-intervention measured serum leptin levels via ELISA kit (Bio vendor, Czech Republic), while Dexa Lexxos Digital (2D model, USA) determined femur neck BMD. SPSS 20 software, dependent/independent samples t-tests, and Pearson correlation were used for data analysis.
Results: TRX training significantly increased femur neck BMD, reducing serum adiponectin levels versus the control group (P = 0.001 and P = 0.01, respectively). Compared to baseline, TRX training raised femur neck BMD and lowered serum leptin levels (P = 0.000 and P = 0.01, respectively). A significant inverse correlation emerged between femoral neck BMD and serum leptin levels in women with osteopenia (P = 0.00).
Conclusion: Findings suggest that TRX resistance training holds promise for enhancing the rehabilitation of women with osteopenia
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