Introduction: Epithelial–mesenchymal transition (EMT) phenomenon in cancer cells is one of the most sensitive stages in metastasis. In addition, it has been shown that many molecular factors and cellular signals, including the Sox2 gene, are involved in arising EMT, which together cause EMT. The phenomenon of EMT in cancer cells is one of the most sensitive stages in metastasis. Expression of Sox2 gene as an effective gene in altering aggressive behavior of cancer cells has not been studied in patients with gastric cancer. Materials and methods: RNA was extracted from 50 tumoral and 50 normal samples from patients with gastric cancer. Then cDNA synthesis was performed on the extracted RNAs. Primers for the target genes were designed and synthesized. Quantitative real-time PCR was performed to determine the relative expression of the studied genes and the relationship between the clinic pathologic parameters and the amount of RNA was analyzed by Pearson correlation coefficient analysis. Results: mRNA expression of Sox2 in tumor tissue was significantly higher than that in healthy adjacent tumor tissue. The mRNA expression level of Sox2 in tumor tissue was significantly higher than that in healthy adjacent tumor tissue. Also, mRNA levels of Sox2 in tumor tissue indicated a direct and significant correlation with tumor staging (TNM stages) (τ = 0.329, P = 0.02). In addition, there was no significant correlation between mRNA levels of Sox2 in tumor tissue and tumor size (τ = 0.138, P = 0.177). Conclusion: Assessment of Sox2 gene expression in the study of genes involved in EMT process in gastric cancer patients and its relation to pathologic, clinical and metastatic findings in gastric cancer is an effective method in the diagnosis of gastric cancer.
Hanieh Moallem Bandani, Fardin Ali Malayeri, Reza Haji Hosseini, Amirnader Emami Razavi, Volume 7, Issue 2 (3-2020)
Abstract
Introduction: H. pylori infection has a strong association with prevalence of iron deficiency and gastric cancer (GC). Cancer cells reprogram the iron metabolism in order to provide required iron. H. pylori is also need iron for its own growth and reproduction. SLC11A2 encodes a member of the solute carrier family 11 protein family which involved in transportation of divalent metals and iron absorption. We evaluated the relative expression of SLC11A2 in patients with GC and its relation to H. pylori infection and pathological characteristics of tumor. Materials and methods: Forty-five patients with GC were involved in this research of whom 24 patients have been infected with H. pylori. Relative expression of SLC11A2 gene was estimated by quantitative real-time PCR. The relationship of SLC11A2 expression change with Pathological characteristics such as size and grade of tumor cells, lympho-vascular and perineural invasion and clinical stage of disease were evaluated in both infected and uninfected patients. Results: SLC11A2 relative expression was significantly higher (P =0.026) in GC patients infected with H. pylori (11.33 ± 5.22) in comparison to those without infection (2.56 ± 0.65). Although it was not statistically significant, the expression of SLC11A2 in all participants was higher at higher stages (III &IV) of disease (9.84 ± 4.35) in comparison to those with lower stages (2.54 ± 0.75). However, among the patients infected with H. pylori, SLC11A2 expression was significantly (P= 0.027) upregulated in the higher stages of disease (16 ± 7.6) compare to the lower stages (1.8 ± 1.06). Conclusion: SLC11A2 is probably a target gene for H. pylori in order to supply its need to iron. The relative expression changes of SLC11A2 in GC patients were associated with the infection of H. pylori, and pattern of its association with the prognosis of the disease changes in the presence and absence of infection with H. pylori.
