en Clinical Biochemistry Clinical Biochemistry پژوهشي Research <span style="font-size:9pt"><span minion="" pro="" style="font-family:"><b>Introduction</b>: Diabetes causes the body&rsquo;s metabolic balance to be in disarray, which damages organs such as the kidneys due to their high metabolic activity. Tissue damage is caused by oxidative stress and chronic inflammation. Folate may help protect against diabetes complications by reducing oxidative stress, regulating homocysteine and supporting vascular and kidney health. Thus, this study was designed to investigate the effects of daily folate supplementation on glycaemic control, oxidative stress and renal dysfunction in streptozotocin-induced diabetic rats.</span></span><br> <span style="font-size:9pt"><span minion="" pro="" style="font-family:"><b>Materials & Methods</b><b><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">: </span></span></b>Eighteen male Wistar rats were randomly divided into three groups. Diabetes was induced by a single intraperitoneal injection of streptozotocin. The first group included healthy control rats, and the second included untreated diabetic rats. The third group received daily IP injections of folate for four weeks. Fasting blood glucose was measured using a glucometer. Oxidative stress was measured in the renal tissue and serum by determining the levels of malondialdehyde (MDA) and total oxidant status (TOS), respectively. Renal histopathological changes were examined by haematoxylin and eosin staining. The data were analysed using one-way ANOVA followed by Dunnett&rsquo;s post hoc test. p < 0.05 was considered statistically significant.<span lang="FA" dir="RTL" style="font-family:"Times New Roman","serif""></span></span></span><br> <span style="font-size:9pt"><span minion="" pro="" style="font-family:"><b>Results</b><b><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">: &nbsp;</span></span></b>The results showed that folate reduced renal tissue MDA and serum triglyceride levels. Fasting blood glucose and serum TOS showed a decreasing trend, but the changes were not statistically significant. The tissue analysis showed slight improvements in kidney structure, but no full recovery was observed.</span></span><br> <span style="font-size:9pt"><span minion="" pro="" style="font-family:"><b>Conclusion</b><b><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">: &nbsp;</span></span></b>These data suggest that folate supplementation may provide some protection against renal injury due to diabetes. The effect on glycaemic control and full recovery of renal tissue appeared to be limited. These findings highlight the need for further studies to determine whether the combination of folate with other therapies could improve glycaemic control and promote tissue repair more effectively</span></span> Diabetes, Oxidative stress, Folate, Kidney function, Malondialdehyde 19 29 http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-1519-4&slc_lang=en&sid=1 Rahman Mohammadi 10031947532846008424 0009-0005-7497-9607 No Department of Comparative Biosciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Akram Vatannejad vatannejad@ut.ac.ir 10031947532846008425 10031947532846008425 Yes Department of Comparative Biosciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Asma Kheirollahi 10031947532846008426 10031947532846008426 No Department of Comparative Biosciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Sara Shokrpoor 10031947532846008427 10031947532846008427 No Department of Pathology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran AmirHossein RahimBakhsh 10031947532846008428 0009-0003-8524-2518 No Department of Comparative Biosciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Tahmoures Sedighi Pashaki 10031947532846008429 0009-0003-6479-7151 No Department of Comparative Biosciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran