en Clinical Biochemistry Clinical Biochemistry پژوهشي Research <span style="font-size:9pt"><span style="font-family:&quot;Minion Pro&quot;,&quot;serif&quot;"><b>Introduction</b>: Diabetes can cause metabolic disorders and the liver is an organ at risk due to its crucial role in glucose and lipid metabolism. Oxidative stress and inflammation play key roles in the development of diabetic liver disease. Folate, an antioxidant water-soluble B-vitamin, might prove beneficial in the treatment of these conditions through decreasing oxidative stress and regulating the metabolism of homocysteine. The present study was designed to investigate the effect of folate supplementation on glucose metabolism, redox status and liver dysfunction in streptozotocin-induced diabetic rats.</span></span><br> <span style="font-size:9pt"><span style="font-family:&quot;Minion Pro&quot;,&quot;serif&quot;"><b>Materials & Methods</b><b><span style="font-size:11.0pt"><span style="font-family:&quot;Times New Roman&quot;,&quot;serif&quot;">: </span></span></b>Adult male Wistar rats (200 +/- 20 g) were randomly divided into three groups (n=6): control, STZ-induced diabetic and folate-treated diabetic. Diabetic status in the two diabetic groups was induced by a single intraperitoneal (i.p.) injection of streptozotocin (55 mg/kg). Folate (15 mg/kg, i.p.) was administered once daily for 4 weeks in the therapy group. At end of the study, fasting blood sugar (FBS), serum liver enzymes, hepatic MDA, SOD activity and TAC were determined. Data were analyzed by one-way ANOVA and Dunnett&rsquo;s post hoc test.<span lang="FA" dir="RTL" style="font-family:&quot;Times New Roman&quot;,&quot;serif&quot;"></span></span></span><br> <span style="font-size:9pt"><span style="font-family:&quot;Minion Pro&quot;,&quot;serif&quot;"><b>Results</b><b><span style="font-size:11.0pt"><span style="font-family:&quot;Times New Roman&quot;,&quot;serif&quot;">: &nbsp;</span></span></b>Folate supplementation resulted in mild improvement in body weight, FBS, ALT and AST levels in diabetic rats. However, these changes were not significant when compared to untreated diabetic animals. There were no significant changes in the hepatic levels of MDA and TAC, and a mild non-significant increase in SOD activity after the administration of folate.</span></span><br> <span style="font-size:9pt"><span style="font-family:&quot;Minion Pro&quot;,&quot;serif&quot;"><b>Conclusion</b><b><span style="font-size:11.0pt"><span style="font-family:&quot;Times New Roman&quot;,&quot;serif&quot;">: &nbsp;</span></span></b>Folate exerted small, possibly beneficial effects on metabolic and hepatic oxidative stress in STZ-induced diabetic rats. The slight improvement in antioxidant status, although not statistically significant, suggests that folate may have protective effects in diabetic conditions. Its therapeutic efficacy may be enhanced when used as an adjunct to conventional antidiabetic treatments.</span></span> Diabetes, Oxidative Stress, Liver Function, Folate, Malondialdehyde, Total Antioxidant Capacity, Streptozotocin 49 57 http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-1519-5&slc_lang=en&sid=1 Rahman Mohammadi 10031947532846008535 0009-0005-7497-9607 No Department of Comparative Biosciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Akram Vatannejad vatannejad@ut.ac.ir 10031947532846008534 10031947532846008534 Yes Department of Comparative Biosciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Asma Kheirollahi 10031947532846008536 10031947532846008536 No Department of Comparative Biosciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran