en Clinical microbiology Clinical microbiology پژوهشي Research <div dir="ltr"> <p style="text-align: justify"><strong>Introduction:</strong> Although bacteria producing biofilm are more resistance to antimicrobial agents, biofilm formation can stimulated by sub-minimal inhibitory concentrations (sub-MICs) of some antimicrobial agents. Therefore, we designed present study to investigate the &nbsp;<em>in vitro</em> efficacy of several antibiotics (including ceftazidime, piperacillin, ticarcillin, carbenicillin, aztreonam, meropenem, gentamicin, amikacin and ciprofloxacin) and biocides (including savlon, benzalkonium chloride and chlorohexidin) on biofilm formation in <em>Pseudomonas aeruginosa</em> (<em>P. aeruginosa</em>) isolates.</p> <p style="text-align: justify"><strong>Materials and methods:</strong> A total of 10 uropathogenic <em>P. aeruginosa</em> isolates were collected from Mostafa Khomaini Hospital in Ilam. The isolates were evaluated for MIC, biofilm formation ability and finally the effect of different concentration of antimicrobial agents on the biofilm formation.</p> <p style="text-align: justify"><strong>Results:</strong> Our finding demonstrated that all antimicrobial agents except gentamicin, aztreonam, and savlon were able to induce biofilm formation at sub-MICs. Moreover, savlon was the best agents for encountering biofilm formation in <em>P. aeruginosa</em>.</p> <p style="text-align: justify"><strong>Conclusion:</strong> Some antimicrobial agents are able to induce biofilm formation at sub-MICs. Biofilm formation inducement depended on antimicrobial agents, strains, and matrix composition.</p> </div> Pseudomonas aeruginosa, Biofilm, Biofilm formation inducement 26 30 http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-194-1&slc_lang=en&sid=1 Saeed Hemati v.hatami1392@gmail.com 10031947532846002698 10031947532846002698 No Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran Nourkhoda Sadeghifard v.hatami1392@gmail.com 10031947532846002699 10031947532846002699 No Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran Sobhan Ghafurian v.hatami1392@gmail.com 10031947532846002700 10031947532846002700 No Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran Farajolah Maleki v.hatami1392@gmail.com 10031947532846002701 10031947532846002701 No Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran Zahra Mahdavi v.hatami1392@gmail.com 10031947532846002702 10031947532846002702 No Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran Azar Hassanvand v.hatami1392@gmail.com 10031947532846002703 10031947532846002703 No Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran Hassan Valadbeigi v.hatami1392@gmail.com 10031947532846002704 10031947532846002704 No Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran Samir Hemati v.hatami1392@gmail.com 10031947532846002705 10031947532846002705 No Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran Vahid Hatami v.hatami1392@gmail.com 10031947532846002706 10031947532846002706 Yes Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran