en Genetics Genetics پژوهشي Research <div> <p><strong>Introduction:</strong> <em>Toxoplasma gondii </em>(<em>T. gondii</em>) is distributed worldwide and infects most species. The&nbsp;serious&nbsp;incidence and severe or&nbsp;fatal&nbsp;injury&nbsp;caused by <em>T. gondii</em> infection clearly indicates&nbsp;the necessity&nbsp;for&nbsp;the event&nbsp;of a&nbsp;vaccine. The current study goals were to evaluate serological applications of Toxoplasma gondiirhoptry protein 1 (ROP1) antigen.</p> <p><strong>Materials and methods:</strong> We created&nbsp;a polymer&nbsp;vaccine by using the eukaryotic plasmid, pROP1. Purification by one-step metal affinity chromatography allowed recovery of milligram amounts of purified recombinant proteins per liter of culture. The&nbsp;quality&nbsp;of this&nbsp;matter&nbsp;for&nbsp;diagnosing&nbsp;of human infections was provided and tested on 77 serum samples which were obtained during routine diagnostic tests. A panel of 20 serum samples from patients with acute toxoplasmosis was compared to a panel of 35 serum samples from individuals with chronic toxoplasmosis.</p> <p><strong>Results: </strong>Results of the study indicated that antibodies detected from patients with acute and chronic infections were 96% and 17%, respectively, by using of pROP1 recombinant antigen.</p> <p><strong>Conclusion: </strong>According to the present study an immunoglobulin G antibody against ROP1 antigen is made&nbsp;throughout&nbsp;the acute stage of toxoplasmosis infection, but not in the chronic phase of toxoplasmosis.</p> </div> Toxoplasma gondii, ROP1, Recombinant antigen, Acute toxoplasmosis 39 41 http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-168-2&slc_lang=en&sid=1 Fatemeh Keshavarzi gol.keshavarzi@gmail.com 10031947532846002585 10031947532846002585 Yes Department of Biology, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran Parviz Ashtari parvizashtari@gmail.com 10031947532846002586 10031947532846002586 No Radiation Application Research School, Nuclear Science and Technology Research Institute, Tehran, Iran Pirooz Ebrahimi gol.keshavarzi@gmail.com 10031947532846002587 10031947532846002587 No Sapienza University of Rome, Yas Women Hospital, Tehran