en Genetics Genetics پژوهشي Research <div><strong>Introduction:</strong> Cervical cancer is the third most common tumor among women. Surgery, radiotherapy, and chemotherapy are common treatments, however high stage tumors have frequently poor prognosis. Nowadays, the epigenetic reversion introduced as an efficient strategy of treatment of cervical cancer. In the process, inhibitors of DNA methyltransferase (DNMT) induce re-expression of tumor suppressor genes. Among these inhibitors, disulfiram (DSF) has been suggested as non-nucleoside analogous. In this research, we evaluated the epigenetic effect of DSF on demethylation of the tumor suppressor gene, RASSF1A, in Hela cell line.<br> <strong>Materials and methods: </strong>Hela cells were cultured and treated with different doses from 2.5 to 37.5&micro;M during 24, 48 and 72 hours. MTT assay was carried out to find half maximal inhibitory concentration (IC₅₀). The methylation specific PCR (MSP) assay was applied to evaluate methylation pattern.<br> <strong>Results: </strong>The IC₅₀ of DSF was determined at the 2.5, 12.5, and 15&micro;M after72 hours. The MSP results showed partial demethylation at mentioned concentrations after 72h but unmethylated band was not observed after 24h.<br> <strong>Conclusion: </strong>Our findings indicated that, IC₅₀ of DSF exerted a biphasic effect in Hela cell line and at least 72 hours treatment is needed for the epigenetic reversion of DSF on RASSF1Ain Hela cell line.</div> Disulfiram, RASSF1A, Epigenetic reversion, Methylation, Cervical cancer 8 13 http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-303-1&slc_lang=en&sid=1 Seyedeh naghme Noorirad naghmenoorirad@gmail.com 10031947532846002831 10031947532846002831 No Department of Anatomical science, Faculty of Medicine, Babol University of Medical Sciences Mohsen Pourghasem Mpourghasem@hotmail.com 10031947532846002832 10031947532846002832 No Department of Anatomical science, Faculty of Medicine, Babol University of Medical Sciences Farideh Feizi faridehfeizi@yahoo.com 10031947532846002833 10031947532846002833 No Department of Anatomical science, Faculty of Medicine, Babol University of Medical Sciences Zeinab Abedian zeinab_abedian@yahoo.com 10031947532846002834 10031947532846002834 No Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran Masoumeh Ghasemi MSm.ghasemi@gmail.com 10031947532846002835 10031947532846002835 No Cellular and Molecular Biology Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran Zahra Babazadeh zbabazadeh@gmail.com 10031947532846002836 10031947532846002836 Yes Department of Anatomical Sciences, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran Navid Rabiee 10031947532846002837 10031947532846002837 No Department of Chemistry, Shahid Beheshti University, Tehran, Iran