Journal of Basic Research in Medical Sciences
مجله ی تحقیقات پایه در علوم پزشکی
Journal of Basic Research in Medical Sciences
Medical Sciences
http://jbrms.medilam.ac.ir
1
admin
2383-0506
2383-0972
doi
en
jalali
1396
10
1
gregorian
2018
1
1
5
1
online
1
fulltext
en
Cucurbita maxima (Pumpkin) seeds: Scolicidal activity and preventive efficacy of its extract on experimental hydatidosis in mice
Parasitologiy
Parasitologiy
پژوهشي
Research
<div><strong>Introduction: </strong>Hydatid Disease (HD), a helminth infection with various clinical complications is a serious economic and public health concern around the world. Nowadays, different agents have been broadly applied as scolicids; these agents have several side effects on patients. Therefore, the aims of this experimental study were to evaluate the scolicidal activity of Cucurbita maxima seeds methanolic extract (ME) against protoscolices of hydatid cyst in-vitro. Also, an in vivo assay was designed to prove the preventive effects of the extract.<br>
<strong>Materials and methods:</strong> For this work, Two different concentrations (25 and 50 mg/ml) of the C. maxima seeds ME were used in various exposure times (10, 20, 30, 40, 50, and 60 min). Additionally, thirty mice were infected intraperitoneally by injection of 1000 viable protoscolices and were divided into three groups with ten mice in each Group I (treated by Albendazole), group II (received 50 mg/ml of C. maxima seeds ME) and group III (untreated controls).<br>
<strong>Results:</strong> Overall, C. maxima seeds ME exhibited significant scolicidal effects in compared with control group (P<0.05). At a concentration of 50 mg/ml, the mortality percentage increased to 84.7%, 96.1% and 100% after 40, 50 and 60 minutes, respectively. Also, the obtain data in necropsy demonstrated that C. maxima seeds ME have an inhibitory effect on the hydatid cyst development. No hydatid cyst formation observed in mice treated with C. maxima seeds ME (50 mg/ml) versus control group which hydatid cyst occurred in 6 (from 10) mice.</div>
<strong>Conclusion: </strong>The current investigation indicated that applying C. maxima seeds ME could be considered as a potential scolicidal agent due to being economical, safer and non-toxic compared to the reference chemical drugs. However, further studies on probably active compounds and also to the determination of its mode of action on parasites are required.
Hydatid disease, Cucurbita maxima methanolic extracts, Scolicidal, Preventive assay
22
28
http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-127-7&slc_lang=en&sid=1
Arash
Babaei
a.babaei@sheffield.ac.uk
10031947532846002973
10031947532846002973
Yes
Department of Biology, Faculty of Sciences, Malayer University, Malayer, Iran
Arash
Jafari
shamsi_ilam@yahoo.com
10031947532846002974
10031947532846002974
No
Department of Pathobiology, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran
Mohammad
Asadpour
shamsi_ilam@yahoo.com
10031947532846002975
10031947532846002975
No
Department of Pathobiology, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran
Morteza
Shamsi
shamsi_ilam@yahoo.com
10031947532846002976
10031947532846002976
No
Department of Parasitology, Faculty of Paramedical Sciences, Ilam University of Medical Sciences, Ilam, Iran