Journal of Basic Research in Medical Sciences
مجله ی تحقیقات پایه در علوم پزشکی
Journal of Basic Research in Medical Sciences
Medical Sciences
http://jbrms.medilam.ac.ir
1
admin
2383-0506
2383-0972
doi
en
jalali
1393
3
1
gregorian
2014
6
1
1
1
online
1
fulltext
en
Investigating antimicrobial activity of hydroalcoholic extract and essential oil of Tymbra spicata against some pathogenic bacteria
Clinical microbiology
Clinical microbiology
پژوهشي
Research
<p>Introduction: Thymbra spicata (T. spicata) is a conventional medicinal herb which is commonly used in the folklore medicine system of Iran. Aim of the study was to assess the antibacterial activity of the essential oil and hudroalcholic extract of the plant T. spicata on some important bacteria. Materials & methods: The essential oil and extract of T. spicata were obtained by hydrodistillation and hydroalcoholic methods, respectively. Different concentrations of essential oil and hydroalcholic extract of the medicinal herb, T. spicata, were evaluated for their antibacterial activities against ten pathogenic bacteria (4 Gram-positive and 6 Gram-negative) by agar disc diffusion and macro Broth dilution method Results: All the bacteria showed a profound susceptibility to the essential oil and hydroalcholic extracts of the T. spicata. The essential oil was shown to be more effective against the examined bacteria. The zone of inhibition of the essential oil shown to be maximum against E. faecalis (28 mm) and M. morganii (30 mm). The hydroalcholic extract of T. spicata, showed the highest antibacterial activity against S. aureus (Gram-positive) and E. coli (Gram-negative) with the zone of inhibitions, 19 mm and 15 mm, respectively. The minimum inhibitory concentration (MIC) values for the essential oil and hydroalcholic extracts ranged from 7-60μg/ mL and 5-19 mg/ml, respectively against the tested bacteria. Conclusion: This study revealed the potential using of T. spicata as a source of antibacterial agents that could be effectively used for medicinal and pharmaceutical purposes.</p>
Tymbra spicata, Essential oil, hydroalcholic extract, antibacterial activity
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7
http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-26-1&slc_lang=en&sid=1
Salar
Bakhtiyari
bakhtiyaribio@gmail.com
10031947532846001699
10031947532846001699
No
Ilam University of Medical Sciences
Somaieh
Sabzali
bakhtiyaribio@gmail.com
10031947532846001700
10031947532846001700
No
Ilam University of Medical Sciences
Arman
Rostamzad
bakhtiyaribio@gmail.com
10031947532846001701
10031947532846001701
No
Ilam University of Medical Sciences
Gholam
Basati
basati-gh@medilam.ac.ir
10031947532846001702
10031947532846001702
Yes
Ilam University of Medical Sciences