Journal of Basic Research in Medical Sciences
مجله ی تحقیقات پایه در علوم پزشکی
Journal of Basic Research in Medical Sciences
Medical Sciences
http://jbrms.medilam.ac.ir
1
admin
2383-0506
2383-0972
doi
en
jalali
1398
6
1
gregorian
2019
9
1
6
4
online
1
fulltext
en
The effects of ginger extract on cyclooxygenase-2 gene expression in polycystic ovary syndrome rats
Physiology
Physiology
پژوهشي
Research
<div><strong>Introduction: </strong>Polycystic ovary syndrome (PCOS) is one of the most common endocrine disorders, can be associated with problems, such as hyperandrogenism, chronic anovulation and infertility. Ginger and its active components, have powerful anti-inflammatory and antioxidant effects. Cyclooxygenase-2 (COX-2) is the enzyme in the biosynthesis of prostaglandins and operates as an inducible enzyme with a number of inflammatory stimuli like PCOS. In this research, we evaluated the effects of ginger extract on ovarian COX-2 gene expression and reproductive improvement in PCOS rats.<br>
<strong>Materials and methods:</strong> After induction of PCOS (by Estradiol Valerate injection), the rats divided into, control, PCOS control, PCOS treated with ginger extract (150 and 300 mg/kg) groups. At the end of treatment period, biochemical factors were measured by ELISA kits and histological assessment was done. Then RNA isolation and cDNA synthesis of ovarian tissues was performed. The data were analized by one way ANOVA ,followed by Tucky test and gene expression data were evaluated by using ΔΔCT method. Statistical significance level was set at P<0.05.<br>
<strong>Results:</strong> Administration of ginger extract to PCOS treated groups, led to improved gonadotropin, sex steroids and ovarian functioning. In addition, treatment of the PCOS group with 300 mg/kg of ginger extract caused to reduce COX-2 gene expression significantly (P<0.01).<br>
<strong>Conclusion: </strong>Ginger extract can act as a natural anti-inflammatory agent, and can use as a replacement of conventional synthetic anti-inflammatory drugs, in the chronic inflammatory conditions like PCOS.</div>
Ginger, Cyclooxygenase, PCOS,Gene expression
37
44
http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-368-1&slc_lang=en&sid=1
Parisa Sadat
Pournaderi
parisa.pournaderi@gmail.com
10031947532846003509
10031947532846003509
No
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Parichehreh
Yaghmaei
parichehrehyaghmaei@gmail.com
10031947532846003510
10031947532846003510
No
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Seyed Hossein
Hejazi
seyyedhosein.hejazi@gmail.com
10031947532846003511
10031947532846003511
Yes
Skin Disease and Leishmaniasis Research Center, Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan , Iran
Zahra
Noormohammadi
nourmohamadi.zahraa@gmail.com
10031947532846003512
10031947532846003512
No
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Hamid Reza
Khodaei
khodaei.hr@gmail.com
10031947532846003513
10031947532846003513
No
Department of Animal Sciences, Islamic Azad University, Golpayegan Branch, Isfahan, Iran