en Biochemistry Biochemistry پژوهشي Research <div><strong>Introduction</strong>: In the current study we designed a sophisticated drug delivery nanoparticle to control the methylprednisolone succinate delivery rate and affect the cancer cell growth in culture condition.<br> <strong>Materials and methods: </strong>Magnetic nanoparticles were synthesized through co-precipitation method. Fe₃O₄ was first prepared via co-precipitation method and then its surface was functionalized with polyamidoamine (PAMAM) nanodendrimer. PAMAM synthesis trait was detected via FT-IR and SEM methods. Methylprednisolone drug was loaded on PAMAM@Fe₃O₄ and its effect against cancer cell lines was studied. In order to slow down drug release rate from nanoparticles, PAMAM@Fe₃O₄ were coated with trimethylchitosan (TMC) after drug loading. &nbsp;Performance of PAMAM@Fe₃O₄@TMC nanoparticles loaded with mmethylprednisolone, were evaluated against two cell lines to detect the cytotoxic and apoptotic effects by invert light scanning microscopy, immunoassay, and LDH cytotoxicity Kit.<br> <strong>Results: </strong>According to SEM, image size of Fe₃O₄ was 4.79-6.37nm, which is smaller than nanodendrimer (6.30-43.67 nm). FT-IR spectrum for ester bond Methylacrylate @ Ethylendiamin was obtained to be 1720-1730 cm^-1. FT-IR Spectrums 600 cm^-1, 1000 cm^-1 belong to Fe₃O₄, and Fe₃O₄@ NH₂. Also, trimethyl chitosan coated Nanoparticle @ Drug, smearing trimethyl chitosan with Glutaraldehyde, created cross link between TMC monomer at low drug doses in each complete nanoparticle, gave confidence drug side effect, therefore, this nanoparticle could be safe for future cancer therapy.<br> <strong>Conclusion: </strong>The results showed that drug delivery via PAMAM@Fe₃O₄ nanoparticle reduces cell viability in vitro condition.&nbsp;</div> PAMAM dendrimer, Fe3O4, Trimethylchitosan, Cytotoxicity, Magnetic, Drug delivery 6 13 http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-168-6&slc_lang=en&sid=1 Leila Sofi Maryo Sofi Maryo gol.keshavarzi@gmail.com 10031947532846003023 10031947532846003023 Yes Department of Biochemistry, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran Nahid Haghnazari n.haghnazari@gmail.com 10031947532846003024 10031947532846003024 No Department of Biochemistry, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran Fatemeh Keshavarzi fkeshavarzi@iausdj.ac.ir 10031947532846003025 10031947532846003025 No Department of Biology, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran Hassan Zhaleh 10031947532846003026 10031947532846003026 No Substance Abuse Prevention Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran Farzad Seidi 10031947532846003027 10031947532846003027 No Department of Chemistry, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran