Journal of Basic Research in Medical Sciences
مجله ی تحقیقات پایه در علوم پزشکی
Journal of Basic Research in Medical Sciences
Medical Sciences
http://jbrms.medilam.ac.ir
1
admin
2383-0506
2383-0972
doi
en
jalali
1398
10
1
gregorian
2020
1
1
7
1
online
1
fulltext
en
Prevalence of mutations in V Leiden and prothrombin genes in women with recurrent pregnancy loss: A retrospective study on Iranian Azeri women
Genetics
Genetics
پژوهشي
Research
<div><strong>Introduction:</strong> The thrombophilia is one of the most important cause of maternal thromboembolism, which is associated with recurrent pregnancy loss (RPL) Risk. The aim of present study was to investigate prevalence of prothrombin (FII, G20210A) and factor V Leiden (FVL, G1691A) genes mutation, as two important cause of thrombophilia, in Iranian Azeri women with RPL.<br>
<strong>Materials and methods:</strong> The subjects in this retrospective study consisted of 100 women (20-40 years old) with RPL recruited from Iranian Azeri population in East Azerbaijan province, Tabriz in Iran. The genomic DNA was extracted from 5 ml peripheral blood samples using the proteinase K method. The Allele and genotype of FII (G20210A) and FVL (G1691A) mutations were assessed using restriction fragment length polymorphism (RFLP) polymerase chain reaction (PCR) method.<br>
<strong>Results:</strong> Our results showed that the frequency of normal homozygous, heterozygous and mutation homozygous for the FII G20210A and FVL G1691A mutations were equally distributed among Iranian Azeri women with RPL. The genotype distribution in RPL patients was, 99% AA, 1% AG, and 0% GG in both of the mutations.<br>
<strong>Conclusion:</strong> In general, our study showed that the prevalence of FVL (G1691A) and FII (G20210A) mutations is low in the Iranian Azeri women with RPL. However, these mutations can be the important reasons for RPL, and more studies with large sample size are required to determine the exact frequency of FVL (G1691A) and FII (G20210A) mutations in Iranian Azeri women with RPL.
<div><strong>Introduction:</strong> The thrombophilia is one of the most important cause of maternal thromboembolism, which is associated with recurrent pregnancy loss (RPL) Risk. The aim of present study was to investigate prevalence of prothrombin (FII, G20210A) and factor V Leiden (FVL, G1691A) genes mutation, as two important cause of thrombophilia, in Iranian Azeri women with RPL.<br>
<strong>Materials and methods:</strong> The subjects in this retrospective study consisted of 100 women (20-40 years old) with RPL recruited from Iranian Azeri population in East Azerbaijan province, Tabriz in Iran. The genomic DNA was extracted from 5 ml peripheral blood samples using the proteinase K method. The Allele and genotype of FII (G20210A) and FVL (G1691A) mutations were assessed using restriction fragment length polymorphism (RFLP) polymerase chain reaction (PCR) method.<br>
<strong>Results:</strong> Our results showed that the frequency of normal homozygous, heterozygous and mutation homozygous for the FII G20210A and FVL G1691A mutations were equally distributed among Iranian Azeri women with RPL. The genotype distribution in RPL patients was, 99% AA, 1% AG, and 0% GG in both of the mutations.<br>
<strong>Conclusion:</strong> In general, our study showed that the prevalence of FVL (G1691A) and FII (G20210A) mutations is low in the Iranian Azeri women with RPL. However, these mutations can be the important reasons for RPL, and more studies with large sample size are required to determine the exact frequency of FVL (G1691A) and FII (G20210A) mutations in Iranian Azeri women with RPL.</div>
</div>
Mutation, Factor V Leiden, Prothrombin, Recurrent pregnancy loss
22
27
http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-452-1&slc_lang=en&sid=1
Elham
Abbaszadeh
abbaszadeh.elham@gmail.cpm
10031947532846004719
10031947532846004719
No
Department of Genetics, Tabriz Branch, Islamic Azad University, Tabriz, Iran
Seyyed Ali
Rahmani
rahmaniseyedali@yahoo.com
10031947532846004720
10031947532846004720
Yes
Department of Molecular Biology, Ahar Branch, Islamic Azad University, Ahar, Iran
Shahla
Danaei Mehrabad
danaei.shahla@yahoo.com
10031947532846004721
10031947532846004721
No
Department of Gynecology, Eastern Azerbaijan ACECR ART Center, Eastern Azerbaijan Branch of ACECR, Tabriz, Iran and