en Microbiology Microbiology پژوهشي Research <div><strong>Introduction</strong>: The <em>Enterococcus faecalis (E. faecalis)</em> is the one of the pathogenic bacteria that become famous and considerable in the recent years. Here we tried to do typing the <a name="_Hlk54454297"><em>E. faecalis</em> </a>isolates to provide advantageous information that can help us to understand epidemiological communication between the <em>E. faecalis</em> isolates.<br> <strong>Materials and methods</strong>: One hundred &nbsp;<em>E. faecalis</em> were isolated from urine samples of Imam Khomeini Hospital, Ilam, Iran. Afterwards, all isolates were confirmed by the phenotyping method and then for more certainty, every isolates were authenticated by PCR analysis of 16sRNA gene. Eventually, all isolates were considered as <em>E. faecalis.</em> For Multilocus Sequence Typing (MLST), 7 housekeeping genes were used to gain MLST scheme for epidemiological study. In addition, to determine various type of <em>E. faecalis</em> pubmlst database was selected and the MLST analysis was done based on recommended instruction by the pubMLST.org.<br> <strong>Results</strong>: The disk diffusion results demonstrated that fifty-four out of one hundred isolates were resistant, four isolates were semi sensitive and forty-two isolates were sensitive to vancomycin. So, 90 isolates were MLST. Using seven structural genes and using pubMLST.org database, different types of <em>E. faecalis</em> were determined. The MLST results demonstrated that 26 different group and Sequence Types (ST) obtained. Our findings demonstrated that the isolates were from different types.<br> <strong>Conclusion:</strong> &nbsp;Accoeding to our results, we couldn&#39;t find any epidemic correlation between the isolates. Given that most of these isolates had resistance to vancomycin, they had low clonal correlation with each other and only had few similar STs pattern.</div> E. faecalis, Multilocus Sequence Typing, Iran 31 35 http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-529-1&slc_lang=en&sid=1 Farzad Mohammadi farzad.mohammadi2010@gmail.com 10031947532846004110 10031947532846004110 No Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran Jalil Vand Yousefi sadeghgudarz@gmail.com 10031947532846004111 10031947532846004111 Yes Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran Naser Harzandi reza_ah61@yahoo.com 10031947532846004112 10031947532846004112 No Department of Microbiology, Karaj Branch, Islamic Azad University, Karaj, Iran Sobhan Ghafourian sobhan.ghafurian@gmail.com 10031947532846004113 10031947532846004113 No Clinical Microbiology Research Center, Ilam University of Medical Sciences, Ilam, Iran