Journal of Basic Research in Medical Sciences
مجله ی تحقیقات پایه در علوم پزشکی
Journal of Basic Research in Medical Sciences
Medical Sciences
http://jbrms.medilam.ac.ir
1
admin
2383-0506
2383-0972
doi
en
jalali
1400
3
1
gregorian
2021
6
1
8
3
online
1
fulltext
en
Identification of extended-spectrum β-lactamase genes in Escherichia coli isolated from patients with urinary tract infection
Microbiology
Microbiology
پژوهشي
Research
<div><strong>Introduction:</strong> Recentlly, resistance to antibiotics has increased and antibiotic-resistant strains producing extended-spectrum beta-lactamases (ESBLs) have emerged among Enterobacteriaceae, mainly in Escherichia coli (<em>E. coli</em>). In this study we aimed to determine phenotypic and genotypic ESBL production in isolated <em>E. coli</em> from women with urinary tract infection (UTI).<br>
<strong>Materials and Methods:</strong> In total, 92 <em>E. coli </em>isolates were collected from patients with UTI. The antimicrobial susceptibility of all <em>E. coli</em> isolates were investigated. Morover, Mast D68C test and polymerase chain reaction (PCR) were used for phenotypic and genotypic investigation of ESBLs in<ins cite="mailto:1" datetime="2021-03-10T08:17"> the</ins> studied isolates.<br>
<strong>Results:</strong> Totally, 92 isolates of <em>E. coli</em> were investigated, <ins cite="mailto:1" datetime="2021-03-10T08:18">among </ins>which 51 (55.4%) isolates were resistant to cefotaxime/ceftazidime. Th<ins cite="mailto:1" datetime="2021-03-10T08:18">e</ins><del cite="mailto:1" datetime="2021-03-10T08:18">i</del>s<ins cite="mailto:1" datetime="2021-03-10T08:18">e</ins> resistante isolates were included in the study. Among the resistant isolates, 40 (78.4%) cases were ESBL producers. Moreover, all <ins cite="mailto:1" datetime="2021-03-10T08:18">the </ins>40 isolates were observed with both CTX-M-15 and CTX-M-14 resistance genes.<br>
<strong>Conclusion: </strong>In general, <del cite="mailto:1" datetime="2021-03-10T08:19">high </del><ins cite="mailto:1" datetime="2021-03-10T08:19">increasing </ins>prevalence of ESBL producer <em><ins cite="mailto:1" datetime="2021-03-10T08:19">E. coil</ins></em> isolates <del cite="mailto:1" datetime="2021-03-10T08:19">of <em>E. coil</em> </del>is a serious problem in the investigated region. Therefore, development of a rapid and simple method is essential <del cite="mailto:1" datetime="2021-03-10T08:21">to </del><ins cite="mailto:1" datetime="2021-03-10T08:21">for the </ins>identification of various ESBL producer isolates.</div>
Urinary tract infection, Escherichia coli, Extended-spectrum β-lactamase, CTX-M
1
7
http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-503-5&slc_lang=en&sid=1
Alireza
Jahantabi
alirezajahantabi@gmail.com
10031947532846004398
10031947532846004398
No
Department of Biological Sciences, Islamic Azad University, Tehran North Branch, Tehran, Iran
Farzaneh
Hosseini
hosseinmicrobiology@gmail.com
10031947532846004399
10031947532846004399
Yes
Department of Biological Sciences, Islamic Azad University, Tehran North Branch, Tehran, Iran
Mohammad
Asgharzadeh
Asgharzadeh.Mohammad@gmail.com
10031947532846004400
10031947532846004400
No
Department of Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
Abbas
Akhavan Sepehi
akhavansepahy@gmail.com
10031947532846004401
10031947532846004401
No
Department of Biological Sciences, Islamic Azad University, Tehran North Branch, Tehran, Iran
Hossein
Samadi Kafil
Samadi.hossein@yahoo.com
10031947532846004402
10031947532846004402
No
Department of Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran