en Medical genetics Medical genetics پژوهشي Research <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times=""><span style="color:black">Introduction: </span></span></b><span new="" roman="" style="font-family:" times=""><span style="color:black">Asthenozoospermia (AZS), sperm immobility, is one of the major cause of men infertility. Evidence suggested that microRNAs (miRNAs) play a critical role in spermatogenesis process. However, the association of miRNAs polymorphisms with idiopathic male infertility remains unknown. Therefore, we investigated correlation between miR-196a2 rs11614913 polymorphism and idiopathic AZS among Iranian Azeri men.</span></span><span lang="EN-GB" new="" roman="" style="font-family:" times=""><span style="color:black"></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times=""><span style="color:black">Materials and Methods</span></span></b><span new="" roman="" style="font-family:" times=""><span style="color:black">: In this study, 50 men with idiopathic AZS (case group) as well as 50 age and ethnically matched healthy men (control group) were enrolled from East Azerbaijan, Iran. The proteinase K method was used to extract the genomic DNA from sperm samples. Finally, genotyping was conducted using tetra-primer amplification refractory mutation system-polymerase chain reaction (Tetra-ARMS PCR) method. </span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times=""><span style="color:black">Results: </span></span></b><span new="" roman="" style="font-family:" times="">The frequency of TT, TC, and CC genotypes were 12%, 54%, and 34%, respectively, in patients with AZS; whereas the figures were 8%, 40%, and 52% in healthy controls, respectively. We found a significant difference between case and control groups in term of CC genotype frequency (P = 0.016). <b><span style="color:black"></span></b></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times=""><span style="color:black">Conclusion: </span></span></b><span new="" roman="" style="font-family:" times=""><span style="color:black">We found a significant correlation between miR-196a2 rs11614913 polymorphism and AZS in Iranian Azeri men.</span></span></span></span></span><br> &nbsp; Asthenozoospermia, miR-196a2, Polymorphism, Tetra-ARMS PCR 25 30 http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-563-2&slc_lang=en&sid=1 Hamed Mohammadzadeh Zangalani hamedmohamadzadeh93@gmail.com 10031947532846007562 10031947532846007562 No Department of Molecular Biology, Tabriz Branch, Islamic Azad University, Tabriz, Iran Seyed Babak Khalilzadeh Kaleybar b_khalifehzadeh@yahoo.com 10031947532846007563 10031947532846007563 Yes Department of Pathology, Tabriz Branch, Islamic Azad University, Tabriz, Iran