Journal of Basic Research in Medical Sciences
مجله ی تحقیقات پایه در علوم پزشکی
Journal of Basic Research in Medical Sciences
Medical Sciences
http://jbrms.medilam.ac.ir
1
admin
2383-0506
2383-0972
doi
en
jalali
1400
12
1
gregorian
2022
3
1
9
2
online
1
fulltext
en
Nociceptive threshold response and alterations of special genes expression during methamphetamine administration and treatment with buprenorphine
Neurology
Neurology
پژوهشي
Research
<div style="border-bottom:solid windowtext 1.0pt; border-top:solid windowtext 1.0pt; border-left:none; border-right:none; padding:1.0pt 0in 0in 0in"><span style="font-size:11pt"><span style="line-height:normal"><span style="tab-stops:445.5pt"><span style="text-autospace:none"><span style="font-family:"Calibri",sans-serif"><b><span style="font-family:"Times New Roman",serif">Introduction: </span></b><span style="font-family:"Times New Roman",serif">Methamphetamine is a nerve stimulant. Buprenorphine has been widely used in the management of various types of pain and reducing addiction side effects. This study aimed </span><span style="font-family:"Times New Roman",serif">to investigate the role of methamphetamine, buprenorphine, or their interaction on analgesic threshold and the expression of protein kinase B (AKT) and glycogen synthase kinase 3 (GSK3b) genes in the lumbar spinal cord of male rats.</span><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="font-family:"Calibri",sans-serif"><b><span style="font-family:"Times New Roman",serif">Materials and Methods:</span></b> <span style="font-family:"Times New Roman",serif">In this experimental study, 56 male Wistar rats (weight </span><span style="font-family:"Times New Roman",serif">200 ± 50 </span><span style="font-family:"Times New Roman",serif">g) were randomly divided into </span><span style="font-family:"Times New Roman",serif">eight</span><span style="font-family:"Times New Roman",serif"> groups: The </span><span style="font-family:"Times New Roman",serif">control group, sham group, </span><span style="font-family:"Times New Roman",serif">methamphetamine group</span><span style="font-family:"Times New Roman",serif">, two </span><span style="font-family:"Times New Roman",serif">buprenorphine groups</span><span style="font-family:"Times New Roman",serif">, two </span><span style="font-family:"Times New Roman",serif">methamphetamines</span><span style="font-family:"Times New Roman",serif"> +</span><span style="font-family:"Times New Roman",serif"> buprenorphine</span><span style="font-family:"Times New Roman",serif"> groups, and deprivation group. The drugs of </span><span style="font-family:"Times New Roman",serif">methamphetamine</span><span style="font-family:"Times New Roman",serif"> and buprenorphine were injected intraperitoneal (i.p) for five days. </span><span style="font-family:"Times New Roman",serif">To measure the </span><span style="font-family:"Times New Roman",serif">analgesic threshold</span><span style="font-family:"Times New Roman",serif">, the </span><span style="font-family:"Times New Roman",serif">Tail-Flick test</span><span style="font-family:"Times New Roman",serif"> was used. Additionally, the </span><span style="font-family:"Times New Roman",serif">real-time PCR technique </span><span style="font-family:"Times New Roman",serif">was applied to evaluate the </span><span style="font-family:"Times New Roman",serif">expression levels of AKT and GSK3b genes</span> <span style="font-family:"Times New Roman",serif">in the lumbar spinal cord of male rats.</span><span style="font-family:"Times New Roman",serif"> A </span><span style="font-family:"Times New Roman",serif">one-way ANOVA </span><span style="font-family:"Times New Roman",serif">test was used to analyze the data.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="font-family:"Calibri",sans-serif"><a name="_Hlk83282040"><b><span style="font-family:"Times New Roman",serif">Results</span></b></a><b><span style="font-family:"Times New Roman",serif">:</span></b><span style="font-family:"Times New Roman",serif"> Intraperitoneal injection of methamphetamine (10 mg/kg) induced analgesia</span><span style="font-family:"Times New Roman",serif"> (P­ <­ 0.05)</span><span style="font-family:"Times New Roman",serif"> and increased the expression of the gene of AKT</span><span style="font-family:"Times New Roman",serif"> (P ­<­ 0.05)</span><span style="font-family:"Times New Roman",serif"> in the lumbar spinal cord of male rats. In addition, the injection of buprenorphine (6 and 10 mg/kg) potentiated the effect of methamphetamine on analgesia </span><span style="font-family:"Times New Roman",serif">(P­ < ­0.01)</span><span style="font-family:"Times New Roman",serif"> and increased the expression of the GSK3b gene </span><span style="font-family:"Times New Roman",serif">(P­ < ­0.05)</span><span style="font-family:"Times New Roman",serif">, whereas the higher dose of buprenorphine reduced the impact of methamphetamine on the expression of AKT gene </span><span style="font-family:"Times New Roman",serif">(P­ <­ 0.05)</span><span style="font-family:"Times New Roman",serif">. Furthermore, the deprivation of methamphetamine, did not alter Tail Flick latency and the expression level of AKT and GSK3b genes.</span></span></span></span></span><br>
<span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="font-family:"Calibri",sans-serif"><a name="_Hlk85713844"><b><span style="font-family:"Times New Roman",serif">Conclusion</span></b></a><b><span style="font-family:"Times New Roman",serif">:</span></b><span style="font-family:"Times New Roman",serif"> Our results indicated a possible reinforcing role of the buprenorphine on the increasing impact of acute methamphetamine injection on the expression of the GSK3b gene and analgesia. </span><b> </b><b><span style="font-family:"Times New Roman",serif"></span></b></span></span></span></span></div>
Methamphetamine, Buprenorphine, Gene expression
25
34
http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-574-1&slc_lang=en&sid=1
Reza
Shahbazi
rezashahbazi1993@yahoo.com
10031947532846004866
10031947532846004866
Yes
Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
Homiera
Hatami Nemati
h.hatami@tabrizu.ac.ir
10031947532846004867
10031947532846004867
No
Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
Hatam
Ahmadi
hahadi@cfu.ac.ir
10031947532846004869
10031947532846004869
No
Department of Basic Sciences, University of Farhangian, Tehran, Iran
Faezehe
Zogoulipour
zoghipourfaezeh@gmail.com
10031947532846004868
10031947532846004868
No
Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran