en Physiology Physiology پژوهشي Research <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Introduction: </span></b><span new="" roman="" style="font-family:" times="">One of the major energy systems for power performance in muscles is glycolysis that is regulated by the levels of Hypoxia-inducible factor-1&alpha; (HIF1), which acts as a transcription factor in response to hypoxia. The purpose of this study was to compare the HIF1 gene polymorphism (rs11549465) between elite and amateur power karate-kas versus non-athletes.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Materials and Methods: </span></b><span new="" roman="" style="font-family:" times="">In this survey, the C/T polymorphism allelic and genotypic distribution of the &ldquo;HIF1 rs11549465&rdquo; gene was detected in 550 healthy Iranian persons who were divided into three groups: elite karate-kas (86 males and 86 females) and amateur karate-kas (100 males and 72 females) versus 206 non-athletes (100 males and 106 females). &nbsp;5 cc blood was taken for DNA extraction and the HIF1 gene T/C polymorphism was determined by PCR from the extracted DNA. Also, RFLP analyses was exerted by electrophoresis separation. Statistical analyses included Chi-Square and multinomial regression tests and data with P < 0.05 were considered to be a significant amount.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Results: </span></b><span new="" roman="" style="font-family:" times="">The distribution of HIF1 C/T genotype in the groups was significantly different in all of subjects (TC: 25.6%; TT: 11.4% and CC: 63%) (&chi;²= 99.889, P = 0.0001), but it was not different between the groups significantly (P > 0.05). Furthermore, multiple regression analysis demonstrated that the genotype of HIF1 was not related to the karate-ka&#39;s athletic status.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Conclusion: </span></b><span new="" roman="" style="font-family:" times="">In Iranian population, the HIF1 gene C/T polymorphism is not related to the karate-ka athletic status.</span></span></span></span><br> &nbsp; HIF1 protein, Polymorphism, Karate, Elite 38 48 http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-396-2&slc_lang=en&sid=1 Mohammad Reza Batavani batavani@iut.ac.ir 10031947532846007568 10031947532846007568 Yes Faculty of Center of Physical Education, Isfahan University of Technology, Isfahan 84156-83111, Iran Sayyed Mohammad Marandi smmarandi2001@yahoo.com 10031947532846007569 10031947532846007569 No Department of Physiology, Faculty of Center of Physical Education, University of Isfahan, Isfahan, Iran Kamran Ghaedi 10031947532846007570 10031947532846007570 No Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Iran Mojtaba Babaei Khorzoghi babaei@iut.ac.ir 10031947532846007571 10031947532846007571 No Faculty of Center of Physical Education, Isfahan University of Technology, Isfahan 84156-83111, Iran Neda Boroushak 10031947532846007572 10031947532846007572 No Department of sports Biomechanics, Sport Sciences Research Institute, Tehran, Iran