Journal of Basic Research in Medical Sciences

en Polymorphism of the Testis-Specific Serine/Threonine Kinase 6 (TSSK6) Gene in Spermatozoa of Asthenozoospermia Patients at Institut Pasteur of Côte d'Ivoire Cellular and molecular biology Cellular and molecular biology پژوهشي Research <div style="text-align: justify;">Introduction:Phosphorylation reactions highlight the essential role of protein kinases in sperm motility. TSSK6 protein is thought to play a crucial role in this process. Indeed, sperm obtained from mice with the Tssk6-/- genotype are unable to carry out fertilization and exhibit decreased motility. This leads us to investigate the causes of asthenozoospermia by searching for polymorphisms in the TSSK6 gene. Therefore, the objective of this study is to identify polymorphisms in the TSSK6 gene in men affected by asthenozoospermia. Material & Methods: The methodology involved direct sequencing of spermatozoa DNA. Thirty ejaculates were analyzed, including 20 from asthenozoospermic men and 10 from normozoospermic men. Spermograms were performed according to WHO procedures. DNA extraction was carried out using the phenol/chloroform method followed by conventional PCR. The amplicons were sequenced using the Sanger method, and the sequences were analyzed with BioEdit software. The data were analyzed using Fisher and Mann-Whitney tests. Results: The results revealed mutations in the TSSK6 gene in both normospermic and asthenozoospermic ejaculates. The synonymous mutations c.690T>C (p.Tyr230Tyr) and c.372C>A (p.Arg124Arg) were the most frequent, occurring at rates of 50% and 33.33%, respectively. Analysis of the mutations using PolyPhen-2 indicated that all mutations observed in normozoospermic samples are benign and would not affect sperm quality. However, only the mutations described in asthenozoospermic samples are predicted to be damaging to the protein. Conclusion: In conclusion, mutations in the TSSK6 gene were observed in infertile men. Deleterious mutations in the TSSK6 protein are associated with asthenozoospermia  </div> Infertility, Male, Gene, Protein, Mutation, TSSK6 66 72 http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-806-1&slc_lang=en&sid=1 Konan Guy Sylvère N'zi guysylverenzi@yahoo.fr 10031947532846006856 0000000214620417 Yes Alassane Ouattara University, Department of Science and Technology, Côte d’Ivoire Jules Eric Hermann Ayekoue ayekouejuleseric@gmail.com 10031947532846006857 10031947532846006857 No Alassane Ouattara University, Department of Science and Technology, Côte d’Ivoire Marie Florence N'guessan miflo2@yahoo.fr 10031947532846006858 10031947532846006858 No Institut Pasteur of Côte d’Ivoire Founzégué Amadou Coulibaly founzegue@yahoo.fr 10031947532846006859 10031947532846006859 No University of San Pedro, UFR Agriculture, fisheries resources and agro-industry, Côte d’Ivoire Allico Joseph Djaman djamanj@yahoo.fr 10031947532846006860 10031947532846006860 No Alassane Ouattara University, Department of Science and Technology, Côte d’Ivoire