en Microbiology Microbiology پژوهشي Research <span style="font-size:11pt"><span style="line-height:150%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Background</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">: &nbsp;Levels of the human epidermal growth factor receptor 2 (HER2) gene are low in normal breast tissue, but half of patients with breast cancer have higher levels of this receptor. The different expression levels of the HER2 gene in normal and malignant cells make this gene an excellent biomarker for therapeutic purposes. In this study, we evaluated the degree of HER2 overexpression in patients, its relationship with age and the occurrence of metastases.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:150%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Methods: </span></span></span></b><span lang="EN" style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">In this retrospective, registry-based and two-center cohort study</span></span></span><span lang="EN" style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">, information of 1500 patients </span></span></span><span style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">with breast cancer recruited at the Shahid Mostafa Khomeini hospital</span></span></span> <span style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">in Ilam province</span></span></span><span lang="EN" style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""> was collected during the years 2020 to 2023. </span></span></span><span style="font-size:12.0pt"><span style="line-height:150%"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:150%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Results:</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""> The possible of metastasis in cancer patients who had the HER2 gene expression was 3 times higher (adjusted OR: </span></span></span><span new="" roman="" style="font-family:" times="">2.82; </span><span style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">95% CI:</span></span></span><span new="" roman="" style="font-family:" times=""> 1.79 &ndash; 3.29</span><span style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">; P=0.001). </span></span></span>&nbsp;<span style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Also,</span></span></span> <span lang="EN" style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Nodes involved</span></span></span><span style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""> (adjusted OR: </span></span></span><span new="" roman="" style="font-family:" times="">2.01</span><span style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">; 95% CI:</span></span></span><span new="" roman="" style="font-family:" times=""> 0.87 &ndash; 3.79</span><span style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">; P=0.03) was significantly associated with increased metastasis.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:150%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></span></b><span style="font-size:12.0pt"><span style="line-height:150%"><span new="" roman="" style="font-family:" times=""> This study showed that the significant prognostic factor of the number of involved lymph nodes and HER2 gene expression is known as a factor that increases the risk of metastasis. Therefore, creating a background for breast cancer screenings can be an important factor for the treatment of the disease and the prevention of metastasis in breast cancer patients.</span></span></span></span></span></span><br> &nbsp; Breast Neoplasms,ERBB2 protein,human,Neoplasm Metastasis 20 27 http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-233-6&slc_lang=en&sid=1 Hassan Nourmohammadi eshafiei1524@gmail.com 10031947532846007481 10031947532846007481 No Non-Communicable Diseases Research Center Ilam University of Medical sciences syedeh negin mirbeigi mirbeigi@medilam.ac.ir 10031947532846007482 10031947532846007482 No Non-Communicable Diseases Research Center Ilam University of Medical sciences, Ilam, Iran Arash Nademi nademiarash@gmail.com 10031947532846007483 10031947532846007483 No Islamic Azad University Ilam Branch, Islamic Azad University Science and Research Branch, Ilam, Iran Elham Shafiei shafiei-e@medilam.ac.ir 10031947532846007484 10031947532846007484 Yes Clinical Research Development Unit, Ayatollah Taleghani Hospital, Ilam University of Medical Sciences, Ilam, Iran