Journal of Basic Research in Medical Sciences
مجله ی تحقیقات پایه در علوم پزشکی
Journal of Basic Research in Medical Sciences
Medical Sciences
http://jbrms.medilam.ac.ir
1
admin
2383-0506
2383-0972
doi
en
jalali
1393
12
1
gregorian
2015
3
1
2
1
online
1
fulltext
en
Effect of maternal anastrozole treatment on ovarian follicle development in neonatal mouse: A morphologic study
Medical Histology
Medical Histology
پژوهشي
Research
Introduction: The origin of neonatal oocyte development is unknown. However, estrogen plays an essential role during development of the female reproductive system. Anastrozole is used as both ovulation stimulating and an anticancer drug. The aim of this study was to evaluate the impact of Anastrozole on follicular development and differentiation in mice.
Materials and methods: In the present study, 30 adult female and 15 adult male mice were used. Then, two female mice (at their sterous cycle) were kept with one male mouse in a cage for mating. After observing the vaginal plug (considered as first day of pregnancy) female mice were divided into two groups of control and experimental. In the experimental group, on the 14th day of pregnancy the mice received anastrozole (50 mg/Kg, i.p. injection). After delivery 16 pups were selected in each group. Then 2, 4 and 7 day pups were studies for primordial, primary and growing follicles number.
Results: According to the morphometric studies, in the 2 day pup, the breakdown was not complete in treatment group. However, the number of primordial, primary and growing follicles of 4 and 7 day pups were not significantly difference in the control and experimental groups.
Conclusion: According to the studies, estrogen is necessary for follicular breakdown and maternal anastrozole can reduce the primordial follicles. However, maternal anastrozole and estrogen depression couldn’t effect on the histology of ovarian follicle in neonatal mouse.
Anastrozole, Ovarian follicles, Neonatal mouse, Histology
42
48
http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-108-1&slc_lang=en&sid=1
Kobra
Afsordeh
Kobra.afsordeh@yahoo.com
1003194753284600954
1003194753284600954
Yes
Department of Medical Sciences, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran.
Tahmineh
Mokhtari
mokhtari.tmn@gmail.com
1003194753284600955
1003194753284600955
No
Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Ardeshir
Moayeri
moayeri46@yahoo.com
1003194753284600956
1003194753284600956
No
Department of Anatomy, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran.
Leila
Roshangar
Lroshangar@yahoo.com
1003194753284600957
1003194753284600957
No
Department of Anatomy, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Hassan
Safari
Akray2008@gmail.com
1003194753284600958
1003194753284600958
No
Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Soudabeh
Houshyari
Shmhoushyari@gmail.com
1003194753284600959
1003194753284600959
No
Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.