en Clinical Biochemistry Clinical Biochemistry پژوهشي Research <span style="font-size:9pt"><span minion="" pro="" style="font-family:"><b>Introduction</b>: Heart disease (HD) is a major global death cause, with adenosine triphosphate metabolites, carnitine, and other biomarkers aiding in timely detection and disease prediction. This study aims to investigate the changes in inosine, hypoxanthine, and carnitine levels in the serum of ischemic HD patients and to examine the relationship of these components with the extent of coronary artery blockage, as well as for the detection of coronary artery disease (CAD).</span></span><br> <span style="font-size:9pt"><span minion="" pro="" style="font-family:"><b>Materials & Methods</b><b><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">: </span></span></b>In this cross-sectional and case-control study, from patients who underwent angiographic assessment for suspected CAD, 40 control subjects, 40 stable angina patients, 40 unstable angina patients, and 40 myocardial infarction patients were selected. Serum samples were collected, and the amounts of inosine, hypoxanthine, and carnitine were measured using an HPLC-UV-visible method. The differences of the three substances in the patients and their clinical importances were analyzed by SPSS V.22 (P < 0.05).<span lang="FA" dir="RTL" style="font-family:"Times New Roman",serif"></span></span></span><br> <span style="font-size:9pt"><span minion="" pro="" style="font-family:"><b>Results</b><b><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">: &nbsp;</span></span></b>The results of this study showed that the levels of inosine and hypoxanthine in patients were higher than those in the control group (P < 0.01), while the level of carnitine was lower than that in the control group (P = 0.04). Conversely, the concentration of carnitine in patients was lower than in the healthy control group, with respective concentrations of 31.84 (28.58-35.62), 34.01 (28.25-37.13), and 33.59 (28.58-38.87) &micro;g/ml.</span></span><br> <span style="font-size:9pt"><span minion="" pro="" style="font-family:"><b>Conclusion</b><b><span style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">: &nbsp;</span></span></b>This study indicated that inosine and hypoxanthine could be effective and rapid discriminators, in comparison with other heart biomarkers, for the timely detection of HD.</span></span><br> <br> &nbsp; Heart disease, Inosine, Hypoxanthine, Carnitine, Cardiovascular diseases 64 76 http://jbrms.medilam.ac.ir/browse.php?a_code=A-10-26-7&slc_lang=en&sid=1 Hossein Nikpour hossein.nikp@gmail.com 10031947532846008049 0009-0007-6371-8043 No Department of Clinical Biochemistry, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran Ali Seidkhani-Nahal seidkhani-a@medilam.ac.ir 10031947532846008050 10031947532846008050 No Department of Clinical Biochemistry, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran Samad Golshani sgolshani@mazums.ac.ir 10031947532846008051 10031947532846008051 No Department of Cardiology, Faculty of Medicine, Cardiovascular Research Center, Mazandaran University of Medical Sciences, Sari, Iran Gholam Basati gholam.basati@gmail.com 10031947532846008052 10031947532846008052 Yes Department of Clinical Biochemistry, School of Medicine, Ilam University of Medical Sciences, Ilam, Iran