eng ilam university of medical sciences Journal of Basic Research in Medical Sciences 2383-0506 2383-0972 2019-01 6 1 1 11 article Barine Innocent Nwiloh bin4him@yahoo.com 1 Augustine Amadikwa Uwakwe 2 Joyce Oronne Akaninwor 3 Department of Biochemistry, Faculty of Science, University of Port Harcourt, Choba, Rivers State, Nigeria Department of Biochemistry, Faculty of Science, University of Port Harcourt, Choba, Rivers State, Nigeria Department of Biochemistry, Faculty of Science, University of Port Harcourt, Choba, Rivers State, Nigeria Introduction: In southern part of Nigeria, root bark of Salacia nitida is use traditionally in the treatment of malaria. Therefore, this study is designed to evaluate the antipyretic and haematomodulatory activities of ethanolic extract from Salacia nitida root bark in Plasmodium berghei-malaria infected mice.   Materials and methods: Fresh roots of S. nitida were collected in February, 2016. Thirty P. berghei-infected mice, divided into five groups containing six mice each were used for the study against another six uninfected control mice. 280, 430 and 580 mg/kg of extract were given to infected mice in groups B, C and D, 4mg/kg of artesunate given to group E mice, while group A and F mice were given 4ml/kg of physiological saline daily for five days. Body temperatures and levels of haematological parameters were evaluated. Results: Results obtained showed significant increase (P < 0.05) in haemoglobin concentration, erythrocyte, leukocyte and platelet counts, and decrease in absolute neutrophil and lymphocyte counts. Body temperatures of experimental mice also decreased significantly (P < 0.05). Conclusion: Therefore, this study suggests that ethanolic extract from S. nitida root bark is effective in treatment of malaria. http://jbrms.medilam.ac.ir/article-1-401-en.pdf Antipyretic Haematological parameters Mice Plasmodium berghei Salacia nitida eng ilam university of medical sciences Journal of Basic Research in Medical Sciences 2383-0506 2383-0972 2019-01 6 1 12 20 article Gelavij Mahmoodi g.mahmoudi@iauksh.ac.ir 1 Sabrieh Amini amini.biology@gmail.com 2 Department of Biology, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran Department of Biology, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran. Introduction: The role of medicinal plants in enhancement of memory and improvement of Alzheimer disease symptoms has attracted researchers’ attention. Genus Salvia (sage) is the largest and most valuable type of herbal medicine from Lamiaceae family, and its therapeutic effects have long been considered. This study investigated the effect of hydroalcoholic extract of Salvia officinalis (S. officinalis) leaves on scopolamine-induced amnesia in adult male mice. Materials and methods: A step-through inhibitory avoidance task was used for memory assessment. Animals received hydroalcoholic extract of Salvia (10, 20 or 40 mg/kg) 30 minutes after administration of scopolamine (1 mg/kg, intraperitoneally) after training and before testing, (based on experimental design). Animals were tested 24 h after the training session, and step-through latency in entering the dark compartment was recorded as passive avoidance memory. The data were analyzed by one-way ANOVA, followed by Tukey test. Statistical significance level was set at P<0.05. Results: The results indicated that administration of scopolamine impaired both consolidation and retrieval of passive avoidance memory. Administration of 40 mg/kg hydroalcoholic extract of Salvia after training, or 20 and 40 mg/kg on the day of experiment ameliorated the effect of scopolamine. Conclusion: The hydroalcoholic extract of Salvia can inhibit scopolamine-induced impairment of passive avoidance memory in mice. http://jbrms.medilam.ac.ir/article-1-379-en.pdf Salvia officinalis Scopolamine Mice Memory impairment eng ilam university of medical sciences Journal of Basic Research in Medical Sciences 2383-0506 2383-0972 2019-01 6 1 21 30 article Ayoub Saeidi gharahcholo@yahoo.com 1 Hassane Zouhal souri348@yahoo.com 2 Akbar Nouri-Habashi Mahnosh_sa@yahoo.com 3 Souri Heydari m83.ahmadi@gmail.com 4 Mahnoosh Salarinahand akbarnori52@gmail.com 5 Mahtab Ahmadi 6 Fatemeh Malekian 7 Exercise Biochemistry Division, Faculty of Physical Education and Sport Sciences, University of Mazandaran, Babolsar, Mazandaran, Iran University of Rennes, M2S (Movement Sports Science Laboratory), Rennes, France Department of Sport and Exercise Physiology, Faculty of Sports Sciences, Urmia University, Urmia, Iran Department of Physical Education and Sport Science, Payame Noor University, Iran Department of Physical Education and Sport Science, Hakim Ferdosi Pardis, Farhangian University, Iran Department of Exercise Physiology, Faculty of Physical Education and Sport Science, Karaj Payam Noor University, Karaj, Iran Professor and Director, Southern University Agricultural Land Grant Campus, Baton Rouge Louisiana, USA Introduction: Aging and low physical activity results in a decrease in Adiponectin and an increase in leptin, which can cause cardiovascular diseases, obesity and diabetes. The aim of present study was to investigate the effect of 8 weeks circuit-resistance training (CRT) with Zataria multiflora (Z. multiflora) supplementation on plasma leptin and adiponectin in postmenopausal women. Materials and methods: 48 untrained postmenopausal women took part in this study. The participants were randomly divided in 4 groups with 12 persons in each: resistance training (RTG), Z. multiflora (ZG), control (CG) and Z. multiflora- resistance training (ZRTG). Resistance training program contained 12 stations (each station 30 second with 35 percent of one maximum repetition) and continued for 8 weeks (3 sessions per week). Participants in the ZG and ZRTG consumed 500 mg of Z. multiflora supplementation daily before breakfast. Blood samples were collected 48 hours before first session and 48 hours after last session to measure plasma levels of leptin and Adiponectin. Results: Significant difference between ZRTG and CG in plasma Adiponectin was observed (P˂0.05). However, there was no significant difference between groups in leptin (P˃0.05). Conclusion: This study indicated that CRT can cause a decrease in leptin and an increase in Adiponectin. Also, when Z. multiflora intervention included in the training program, this effects increased. http://jbrms.medilam.ac.ir/article-1-321-en.pdf Circuit-resistance training Zataria multiflora supplementation Adiponectin Leptin Postmenopausal women eng ilam university of medical sciences Journal of Basic Research in Medical Sciences 2383-0506 2383-0972 2019-01 6 1 31 35 article Manijeh Khalili dr_khalili2000@yahoo.com 1 Gholamreza Kalvandi pezeshk1351@yahoo.com 2 Iraj Shahramian ir_buper@yahoo.com 3 Ali Bazi m.baziali@gmail.com 4 Bita Farsian bita_far@yahoo.com 5 Mahnaz Shahrakipour shahrakipoor@gmail.com 6 Zahedan University of Medical Sciences Ilam University of Medical Sciences Zabol University of Medical Sciences Zabol University of Medical Sciences Zahedan University of Medical Sciences Zahedan University of Medical Sciences Introduction: Constipation is one of the most common gastroenterological disorders worldwide, especially in developing countries. The knowledge toward the influences of constipation on weight-gain and normal growth is inadequate among families and pediatricians. In present study, we aimed to assess weigh-gain pattern in children with constipation during six months of routine therapy. Materials and methods: In this analytical study, our population constituted all the children with age of 1-15 years old admitted to the Gastroenterology section of Children Hospital of Zahedan city during 2015-2017. The constipation diagnosis was based on less than three defecations per week persisting for at least one month. Demographic and growth parameters including height, weight, and BMI, and appetite were recorded for each subject following three months of routine treatment. Results: The mean age of patients under study was 5.67 ± 3.03 years old, and the mean of height was 108.47 ± 19.18 cm. The mean weight and BMI of the children were significantly higher at three months following treatment compared to baseline, however, there was no significant change in neither weight nor BMI regarding individual genders. Appetite improvement was recorded in 60 (57.1%) and 77 (73.3%) of the children at one and three months after treatment, respectively. Conclusion: Therapeutic intervention in children with constipation can effectively improve weight-gain their pattern and appetite. http://jbrms.medilam.ac.ir/article-1-396-en.pdf Constipation Body mass index Appetite Children eng ilam university of medical sciences Journal of Basic Research in Medical Sciences 2383-0506 2383-0972 2019-01 6 1 36 40 article Khadijeh Abdal dr.faribaabdal@yahoo.com 1 Keywan Mortezaee fariba4479@yahoo.com 2 Sara Haidari marzidarvish@yahoo.com 3 Marzieh Darvishi marzidarvish@yahoo.com 4 Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Ilam University of Medical Sciences, Ilam, Iran Department of Anatomy, Faculty of Medicine, Kurdistan University of Medical Sciences, Sanandaj, Iran Department of Oral and Maxillofacial Radiology, Faculty of Dentistry, Ilam University of Medical Sciences, Ilam, Iran Department of Anatomy, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran Introduction: Oral cancer is the sixth most common malignant neoplasm and one of the top ten causes of death worldwide. Dentists’ lack of knowledge about the signs and symptoms of oral cancer is an important for diagnosing the oral cancer accurately and at early stages. In this study, we examined the level of dentists’ awareness toward oral cancer in Ilam, Iran. Materials and methods: A total of 98 dentists (65 males and 33 females) with average age of 35.57±7.9 years were invited to take part in the study and respond to suitably designed questionnaires. Data were collected and a possible relationship between age and knowledge score was further analyzed. Results: The maximum awareness score that respondents could attain was 13. The mean score was 8.14 ± 1.8, which was higher than the national average level. The average scores for men and women were 7.58 ± 2.1 and 8.46 ± 1.5, respectively. But, this difference was not statistically significant (P = 0.1).There was no statistically significant relationship between age and knowledge score (P = 0.3, r = -0.11).In addition, knowledge score in different age groups were compared, and there was no significant difference (P = 0.5). Conclusion: Results of this study indicated that dentists’ awareness about oral cancer is higher than average in Ilam; however, the need for educational programs for increasing the level of knowledge about oral cancer is recommended. http://jbrms.medilam.ac.ir/article-1-367-en.pdf Oral cancer Knowledge Dentist eng ilam university of medical sciences Journal of Basic Research in Medical Sciences 2383-0506 2383-0972 2019-01 6 1 41 48 article Parvin Sadat Hosseini 1 Maryam Rafieirad Rafieirad.m@gmail.com 2 Somayeh Esmaeili 3 MSc student Department of Psychology, Izeh Branch, Islamic Azad University, Izeh, Iran Assistant Professor Department of Biology, Izeh Branch, Islamic Azad University, Izeh, Iran Department of Physical Education and Sport Sciences, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran Introduction: Epileptic seizures are product of abnormal electrical discharges of the brain. Electrical wave productive of epileptic seizure generates disturbances in brain data processing circuits and these patients suffer from memory impairment. In this study, the effect of different doses of oleuropein on the treatment of memory impairment Caused by frequent seizures was in male rats investigated.   Materials and methods: Forty rats were randomly divided into four groups of 10 (the negative control group received normal saline, the positive control group received diazepam 1 mg/kg and the two treatment groups received doses 10 and 20 mg/kg of oleuropein). Thirty minutes after administration of different doses of oleuropein or saline or diazepam, pentylenetetrazole (PTZ) at a dose of 85 mg/kg was injected intraperitoneal into rats, and after creating seizure and animal survival, tests of memory were performed. One-way ANOVA and Tukey's tests were the procedures used to analyze the results. Results: In both tests, the memory of the control group (normal saline recipient) decreased significantly (P<0.001). The administration of 10mg/kg oleuropein shows a significant increase in periodic behavior measurements by maze y (P<0.01). Both doses of 10 and 20 mg/kg increased passive avoidance memory (P<0.001). Conclusion This study shows that the oleuropein has an appropriate anticonvulsant effect and improves the working memory and passive avoidance in epileptic rat and Future studies appear to be necessary to understand further how the mechanism of its effect. http://jbrms.medilam.ac.ir/article-1-414-en.pdf Oleuropein Memory Pentylenetetrazole Rat eng ilam university of medical sciences Journal of Basic Research in Medical Sciences 2383-0506 2383-0972 2019-01 6 1 49 56 article Ciamak Ghazaei ciamakghazaei@yahoo.com 1 Department of Microbiology, University of Mohaghegh Ardabili, Ardabil, Iran Introduction: Molecular transformation of bacterial cells plays a central role in molecular DNA transfer. At present, transformation is carried out either by chemical methods or by electric shock (electroporation). This study tended to investigate the effect of thermal shock factors and different cell treatments on transformation efficiency in E.coli.   Materials and methods: In this method, E.coli cells were treated by different concentrations of peptide CM11 (0.1, 1, 2, 3 and μg/ml) and lysozyme enzyme (40 mg/ml and 80 mg/ml) based on competence by calcium chloride (100 and 200 mM). They also underwent thermal and cold shocks at different temperature intervals. Then, pUC19 plasmid (2.7 kbp) was transferred as a model and separately to the competent bacteria. Results: The results showed that increasing the thermal shock in three temperature intervals (37, 42 and 47°C) with cold shock (0 and 5°C) led to an increase in the number of transformed colonies and it has a significant relationship with transformation (P<0.05). Increased CaCl2 concentration was a positive factor in the amount of transformation, but no significant relationship was found between increased lysozyme concentration and transformation (P>0.05). Increased CM11 peptide concentration showed a significant relationship with transformation (P<0.05). There was no significant relationship between three transformations (P>0.05), while there was a significant relationship between peptide CM11 concentration and lysozyme enzyme concentration in transformation simultaneously (P˂0.05). Conclusion: It can be concluded that increased thermal shock and stability of cold shock at minimum temperature (0°C), as well as increased CaCl2 concentration and increased peptide concentration to 1 μg/ml can increase the number of transformed colonies in E.coli. Finally, peptide concentration and enzyme concentration are introduced as a simple and convenient method for increasing the transformation efficiency. http://jbrms.medilam.ac.ir/article-1-389-en.pdf Transformation Escherichia coli Plasmid Lysozyme Enzyme Peptide CM11 eng ilam university of medical sciences Journal of Basic Research in Medical Sciences 2383-0506 2383-0972 2019-01 6 1 57 66 article Kambiz Moradnejadi kambiz.1358@yahoo.com 1 Ali Torabian ali.torabian@gmail.com 2 Heshmatollah Nourmoradi Ilam_nourmoradi@yahoo.com 3 Sayed Ahmad Mirbagheri mirbagheris@gmail.com 4 Department of Environmental Engineering, School of Energy and Environment, Islamic Azad University, Science and Technology Branch, Tehran, Iran Department of Environmental Engineering, University of Tehran, Tehran, Iran Biotechnology and Medical Plants Research Center, Ilam University of Medical Sciences, Ilam, Iran Department of Environmental Health Engineering, School of Health, Ilam University of Medical Sciences, Ilam, Iran Introduction: Pesticide including lindane has been extensively applied in agriculture as insecticide. It is considered as one of the most significant organic pollutants that have reasoned environmental pollution especially surface and ground waters. In the present study, mineral sand, as a media of slow sand filtration in water treatment, was used to remove lindane from aqueous solutions. Materials and methods: This study was carried out in batch mode. The influences of various parameters such as contact time, pH, lindane content and solution ion strength were investigated on the sorption process. The isotherm and kinetics of the sorption was also determined by the research. Results: The maximum removal efficiency of lindane by sand was achieved at the contact time and pH of 150 min and 4, respectively. The removal efficiency of lindane at abovementioned condition was (28.86% equal to 2.88 µg/g) in the concentration of 100 µg/L. The results of various isotherms and kinetics models analysis also showed that the adsorption process described well by the Freundlich isotherm and pseudo-first-order kinetics models, respectively. Addition of the salt to lindane solution increased the removal efficiency to about 3.5 times. Conclusion: This study showed that the sorption is considered as one of the major parameters of mineral sand as main media in sand filtration of water treatment plant. http://jbrms.medilam.ac.ir/article-1-418-en.pdf Adsorption Sand Lindane Aqueous solution