@article{ author = {Shafiei, Jalil and Heidari, Farideh and Khashen, Elham and Ghandehari‐Alavijeh, Rana and Darmishonnejad, Zahr}, title = {Distinctive deregulation of miR-27a and miR-27b in relapsing remitting multiple sclerosis}, abstract ={Introduction: Previous studies have proposed that microRNAs (miRNAs) expression might be responsible for immunological features associated with multiple sclerosis (MS) pathogenesis. We aimed to elucidate the alternation in miR-27a and miR-27b expression in relapsing-remitting multiple sclerosis (RRMS) patients compared to the healthy subjects. Materials and methods: In this study, the expression levels of miR-27a and miR-27b were evaluated in peripheral blood samples of 60 RRMS patients (30 recurrence patients and 30 patients two months after relapse) and 30 healthy subjects by quantitative real-time PCR. Results: The findings indicated that the expression of miR-27a was significantly decreased in recurring patients (P<0.0001) and two months after relapse patients (P<0.003) in comparison with healthy subjects. Moreover, miR-27b showed down regulation in recurring patients (P<0.001) and two months after relapse patients (P<0.002). Conclusion: The results demonstrated an association between expression of miRNAs studied and RRMS disease during recurrence and two months after relapse. However, further research is warranted to confirm observed associations.}, Keywords = {microRNA, miR-27a, miR-27b, Multiple sclerosis}, volume = {7}, Number = {2}, pages = {1-6}, publisher = {ilam university of medical sciences}, url = {http://jbrms.medilam.ac.ir/article-1-480-en.html}, eprint = {http://jbrms.medilam.ac.ir/article-1-480-en.pdf}, journal = {Journal of Basic Research in Medical Sciences}, issn = {2383-0506}, eissn = {2383-0972}, year = {2020} } @article{ author = {Hemati, Saeed and Shams, Morteza and Rahmatian, Arash and Nourmohammadi, Hasan and Abdoli, Amir and Maleki, Farajolah and Talee, Ghasem and Mahdavi, Zahra and Bastani, Elham and Hatami, Vahid and Kohzadi, Fatemeh}, title = {The patterns of aminoglycoside and fluoroquinolones resistance among uropathogenic Escherichia coli isolates}, abstract ={Introduction: In the study we sought to determine the patterns of regional antibiotic resistances among uropathogenic Escherichia coli (UPEC) isolates. Our finding could be useful for better recognition of regional antibiotic resistances and scheduling a program to control this condition. Materials and methods: In the study, 270 nonduplicate UPEC isolates were examined from urine samples of outpatients with urinary tract infections (UTIs). All isolates were identified by gram staining and standard conventional biochemical tests. Antimicrobial susceptibility test was performed by disk diffusion (Kirby–Bauer) method. The commercial antibiotics disks (PADTAN TEB Co., Iran) were applied in the study, included amikacin (30 μg), gentamycin (10 μg), ciprofloxacin (5 μg), and nalidixic acid (30 μg). Results: Most of the patients were female (221, 81.9%). The highest resistance was observed for nalidixic acid (56%), followed by ciprofloxacin 39.64%. In contrast, the lowest resistance was seen for amikacin (3.90%) and gentamicin (10.04%). Moreover, 19.62% of the isolates were multidrug-resistant (MDR). Conclusion: Amikacin and gentamicin could be chosen as first line antibiotics in treatment of UTIs. Continuous monitoring studies recommended for acquire a suitable regional antibiotic resistance pattern.}, Keywords = {Escherichia coli, UTIs, Antimicrobial susceptibility}, volume = {7}, Number = {2}, pages = {7-12}, publisher = {ilam university of medical sciences}, url = {http://jbrms.medilam.ac.ir/article-1-483-en.html}, eprint = {http://jbrms.medilam.ac.ir/article-1-483-en.pdf}, journal = {Journal of Basic Research in Medical Sciences}, issn = {2383-0506}, eissn = {2383-0972}, year = {2020} } @article{ author = {Rahimnahal, Somayeh and Shams, Morteza and Tarrahimofrad, Hossein and Mohammadi, Yahy}, title = {Analysis to describe the catalytic critical residue of keratinase mojavensis using peptidase inhibitors: A docking-based bioinformatics study}, abstract ={Introduction: Vital catalysts have long been widely used in the food industry, but with new applications in many industries, such as the chemical industry, they have become even more important. In biocatalysis, all parts of the cell, cell extract, purified enzyme, inactive cell, or inactive enzymes are used as catalysts in various processes. Enzymes are essential for various industrial, pharmaceutical, and especially biotechnological processes. Keratinase is produced by various microorganisms in the presence of keratin as a substrate. It mainly targets disulfide bonds. In this study, the biochemical properties of keratinase enzymes derived from Bacillus mojavensis (B. mojavensis) were investigated. Materials and methods: The 3D structure of keratinases from B. mojavensis was created using Modeler software, and the model's validation and refinement indicators, including Prosa, Z-score, and Ramachandran Graph confirmed the high quality of the modeled protein. The PMSF, Pepstatin and leupeptin structures were prepared from the PubChem database server and introduced to the MVD software along with the 3D structure of the keratinase for molecular docking. Results: The binding energies (Eaint#) for the Mojavensis-PMSF, Mojavensis-Pepstatin and Mojavensis-Leupeptin complexes were -71.73, -334.1 and -211.2, respectively. In all three Mojavensis-PMSF, Mojavensis-Pepstatin and Mojavensis-Leupeptin complexes, the Serine 277 keratinase mojavensis formed a hydrogen bond with inhibitors. Serine 295 also interacted with inhibitors in both of Pepstatin and Leupeptin complexes. Glutamic 299 keratinase mojavensis also interacted with PMSF and Leupeptin. All three PMSF, Pepstatin and leupeptin peptidase inhibitors were able to interact with keratinase mojavensis. Conclusion: Docking results showed that Serine amino acids 277 and 295 in the active site of keratinase mojavensis, may play a key role in its catalytic function.}, Keywords = {Bioinformatics, Keratinase, Enzyme, Homology modeling, Docking}, volume = {7}, Number = {2}, pages = {13-28}, publisher = {ilam university of medical sciences}, url = {http://jbrms.medilam.ac.ir/article-1-508-en.html}, eprint = {http://jbrms.medilam.ac.ir/article-1-508-en.pdf}, journal = {Journal of Basic Research in Medical Sciences}, issn = {2383-0506}, eissn = {2383-0972}, year = {2020} } @article{ author = {Nazari, Pari and Yaghmaei, Parichehreh and Rangin, Alireza and Abbasi, Naser}, title = {Influence of Smyrnium cordifolium Boiss extract on the hippocampus Mu-opioid receptors in Mice}, abstract ={Introduction: Agonists of the Mu-opioid receptors (MOR), such as morphine are lengthily used for the treatment of moderate to pain, depression and anxiety. But the dose involved achieving adequate pain relief often elicits multiple unwanted side effects, including addiction and tolerance. Opioids produce their actions at a cellular level by activating MOR. These receptors are distributed throughout the central nervous system (CNS). The target of this study was the effect of Smyrnium cordifolium extract (SCE) on the hippocampus Cornu Ammonis 1 (CA1) aria MOR compared to clonidine. Materials and methods: Extract of the aerial parts S.cordifolium was extracted by Soxhlet method. Addiction was created using the subcutaneous injections of morphine for 7 days. To evaluate the effects of SCE, the mice were divided up 5 groups. The first group (Control) received just morphine. The 2th group received morphine and Clonidine (0.2mg/kg). Groups 3, 4 and 5 were treated morphine and SCE (E1, E2 and E3). In all groups, on the seventh day 30 min after naloxone injection, their brains were perfusion with formaldehyde and removed for immunohistochemical investigation. Results: The present immunohistochemical of the CA1 hippocampus study showed that group E1, there is a significant difference in MOR optical density compared to the control group at the level (P<0.05) and relative to the CLO group at the level (P<0.001). However, in groups E2, and E3 the MOR optical density increased compared to the control group and had a significant difference in level (P<0.001) and did not have a significant difference compared to the CLO group. Conclusion: The study showed that with increasing S.cordifolium extract concentration, the optical density of MOR in the hippocampus increased, and this increase was dose-dependent. This increase in the CA1 hippocampus MOR optical density may be due to endocytosis or desensitization of MOR in neurons.}, Keywords = {Hippocampus, Mu-opioid receptor, Mice, Smyrnium cordifolium}, volume = {7}, Number = {2}, pages = {29-37}, publisher = {ilam university of medical sciences}, url = {http://jbrms.medilam.ac.ir/article-1-497-en.html}, eprint = {http://jbrms.medilam.ac.ir/article-1-497-en.pdf}, journal = {Journal of Basic Research in Medical Sciences}, issn = {2383-0506}, eissn = {2383-0972}, year = {2020} } @article{ author = {Hoseini, Seyed nabiyollah and AbbassiDaloii, Asieh and Ziaolhagh, Seyed Javad and Saeidi, Ayoub}, title = {The effect of aerobic exercise and a synthesized insulin nanocomposite hydrogel on TNF-α and IL-6 in plasma of type 1 diabetic rats}, abstract ={Introduction: Diabetes is one of the leading causes of mortality in the world. The present study aimed to investigate the effect of aerobic exercise training and a synthesized insulin nanocomposite hydrogel on TNF-α and IL-6 in type 1 diabetic rats. Materials and methods: Twenty-five rats were divided into five equal groups of animals each containing five animals, control - healthy, Control-diabetes, Nano-insulin Diabetes, Exercise Diabetes and Nano-insulin Diabetes-Exercise. The exercise training program lasted eight weeks. After the five days of familiarization, exercise time for the exercise groups were as follows: 20 m/min for the first and second weeks, 25 m/min for the third and fourth weeks and 30 m / min for the fifth and sixth weeks. The rats were also given the Nano-insulin supplement. Rats were killed 48 hours after the last training session. Their plasma was taken and used for the analysis of markers. Results: There were significant differences in IL-6 (P<0.001) and TNF-α (P<0.001) between the groups. Conclusion: The results of this study showed that aerobic exercise training along with Nano-insulin supplementation significantly reduced TNF-α and IL-6 in rats with type 1 diabetes and these changes were more in the Nano-insulin Diabetes group.}, Keywords = {Aerobic training, Synthesized insulin nanocomposite hydrogel, TNF-α, IL-6, Type 1 diabetic rat}, volume = {7}, Number = {2}, pages = {38-46}, publisher = {ilam university of medical sciences}, url = {http://jbrms.medilam.ac.ir/article-1-437-en.html}, eprint = {http://jbrms.medilam.ac.ir/article-1-437-en.pdf}, journal = {Journal of Basic Research in Medical Sciences}, issn = {2383-0506}, eissn = {2383-0972}, year = {2020} } @article{ author = {Amani-Shalamzari, Sadegh and AghaAlinejad, Hamid and Shahbazi, Shirin and Alizadeh, Shab}, title = {The Effect of endurance training on expression of miR-21 and its downstream in breast cancer bearing mice}, abstract ={Introduction: Overexpression of oncomir-21 promotes proliferation of breast cancer cells. This study aimed to assess the effect of endurance training on the expression of miR-21 and its downstream, Bcl2 and upstream targets, STAT3 in breast cancer bearing mice. Materials and methods: After orientation to the environment, breast cancer cells, MC4-L2 were injected to mice and they randomly were categorized into two groups, control (n=10) and training (n=10) groups. Training group performed progressive endurance training 5 days per week for 6 weeks and control group did not perform any exercise. Tumor volume was measured by a digital caliper every week. Finally, the mice were sacrificed; tumor tissue was removed and immediately frozen and kept in -70°C. RNA extraction and cDNA synthesis were carried out by trizol reagent and specific kits and level of genes were measured by quantitative real-time PCR.   Results: Endurance training decreased significantly expression of miR-21, STAT3 and Bcl2 (P<0.05). In addition, Tumor volume developed further in control group compared to training group (P<0.05). There was significantly positive correlation (P<0.001) between miR-21 with STAT3(R=0.66) and miR-21 with Bcl2 (R=0.61) Conclusion: Endurance training leads to suppress expression of STAT3/miR-21/Bcl2 signaling pathway, thereby involved in slow tumor growth. Therefore, one of the beneficial effects of endurance training on tumor progression in estrogen dependent mouse model of breast cancer is reducing intrtumor anti-apoptotic genes.}, Keywords = {Estrogen receptor dependent breast cancer, STAT3, Bcl2, miR-21}, volume = {7}, Number = {2}, pages = {47-57}, publisher = {ilam university of medical sciences}, url = {http://jbrms.medilam.ac.ir/article-1-479-en.html}, eprint = {http://jbrms.medilam.ac.ir/article-1-479-en.pdf}, journal = {Journal of Basic Research in Medical Sciences}, issn = {2383-0506}, eissn = {2383-0972}, year = {2020} } @article{ author = {MoallemBandani, Hanieh and AliMalayeri, Fardin and HajiHosseini, Reza and EmamiRazavi, Amirnader}, title = {Upregulation of SLC11A2 in gastric cancer patients with Helicobacter pylori infection}, abstract ={Introduction: H. pylori infection has a strong association with prevalence of iron deficiency and gastric cancer (GC). Cancer cells reprogram the iron metabolism in order to provide required iron. H. pylori is also need iron for its own growth and reproduction. SLC11A2 encodes a member of the solute carrier family 11 protein family which involved in transportation of divalent metals and iron absorption. We evaluated the relative expression of SLC11A2 in patients with GC and its relation to H. pylori infection and pathological characteristics of tumor. Materials and methods: Forty-five patients with GC were involved in this research of whom 24 patients have been infected with H. pylori. Relative expression of SLC11A2 gene was estimated by quantitative real-time PCR. The relationship of SLC11A2 expression change with Pathological characteristics such as size and grade of tumor cells, lympho-vascular and perineural invasion and clinical stage of disease were evaluated in both infected and uninfected patients. Results: SLC11A2 relative expression was significantly higher (P =0.026) in GC patients infected with H. pylori (11.33 ± 5.22) in comparison to those without infection (2.56 ± 0.65). Although it was not statistically significant, the expression of SLC11A2 in all participants was higher at higher stages (III &IV) of disease (9.84 ± 4.35) in comparison to those with lower stages (2.54 ± 0.75). However, among the patients infected with H. pylori, SLC11A2 expression was significantly (P= 0.027) upregulated in the higher stages of disease (16 ± 7.6) compare to the lower stages (1.8 ± 1.06). Conclusion: SLC11A2 is probably a target gene for H. pylori in order to supply its need to iron. The relative expression changes of SLC11A2 in GC patients were associated with the infection of H. pylori, and pattern of its association with the prognosis of the disease changes in the presence and absence of infection with H. pylori.}, Keywords = {SLC11A2, Gastric cancer, H. pylori}, volume = {7}, Number = {2}, pages = {58-68}, publisher = {ilam university of medical sciences}, url = {http://jbrms.medilam.ac.ir/article-1-451-en.html}, eprint = {http://jbrms.medilam.ac.ir/article-1-451-en.pdf}, journal = {Journal of Basic Research in Medical Sciences}, issn = {2383-0506}, eissn = {2383-0972}, year = {2020} } @article{ author = {Mohammadzadeh, Zeinab and Maserat, Elham}, title = {The need for an integrated portal: Effective approach for infodemic management in Covid 19}, abstract ={Dear Editor,   Shortly after the first outbreak in China, the Corona virus has affected many countries around the world. The first confirmed case of the virus in Iran was reported in Qom on February 2020, and then it spread to other cities in Iran. The outbreak has caused many social and economic challenges for the international community; also, there is no complete information about this disease. One of the most important challenges is the prevalence of inaccurate information about the disease, which has many psychological, political, social and economic effects on society. Infodemic or the spread of wrong information about the disease through various social media in the global community is a serious problem for public health. The dissemination of information can severely affect people's behavior and alter the effectiveness of governments’ countermeasures. In this present letter was reviewed integrated portal as a one of the solutions to infodemic management. The use of authentic sources to provide accurate information in the community is one of the best approaches to combat this phenomenon. Timely information sharing and comparison of epidemiological and genomic data on infectious diseases leads to faster and more efficient control of global outbreaks and disease tracking. In spite of all the problems in this area, various solutions have been proposed for this issue. The portal is one of the significant technologies which provide correct and required data for different users. Web portals are increasingly becoming part of modern life. Portals collect uniform information from multiple sources and provide consistent access to multiple information, software and applications from different databases to organizations and community. Portal content is available from a variety of tools such as PCs and smartphones. For example, government web-portals can be used by public and private organizations. Many studies have confirmed the usefulness of portals for managing infectious and chronic diseases, if it is designed according users need. The accurate and comprehensive information sharing with all stakeholders and health organizations such as WHO about Covid-19 is essential for better management of the disease. One of the applications of portals is sharing of accurate and reliable information by responsible authorities for managing this disease. Justice must be respected in presenting results of recent research on Covid 19, and everyone has equal access to this information according to their role in society. By sharing the results of scientific research, the general public are also preparing themselves to fight the disease. In recent days, various applications, soft wares and websites have been provided by the relevant organizations for the effective management of CVD 19. The integration of these technologies into comprehensive portal is essential for a comprehensive management of disease. The portal will be accessible to public, patients, providers and policy makers. It can prepare educational, medical, and research information for users. Some benefits of portal including information gathering from various sources, availability of information for all user groups (general and specific users), uploading information by users according to the access level, designing customized pages by users or groups, decreasing the attendance of patients and public in high risk areas, increasing information security, reducing people's confusion about finding the required information and facilitating communication.}, Keywords = {Infodemic, Covid-19, Portal}, volume = {7}, Number = {2}, pages = {69-70}, publisher = {ilam university of medical sciences}, url = {http://jbrms.medilam.ac.ir/article-1-484-en.html}, eprint = {http://jbrms.medilam.ac.ir/article-1-484-en.pdf}, journal = {Journal of Basic Research in Medical Sciences}, issn = {2383-0506}, eissn = {2383-0972}, year = {2020} }