OTHERS_CITABLE Sugar cane extract: A treatment for atherosclerosis disease? Introduction: Sugar cane is a giant plant of grain products. Regarding the benefits of the use of medications with herbal origin, in the present study the impact of sugar cane extract on atherosclerosis and LDL-c and HDL-c in the serum of hypercholesterolemic rabbits was studied. Materials and methods: 24 adult male New Zealand rabbits race with an average weight of 2 kg were classified into four groups: the control group had a normal diet, Sham group and the 1 , 2 experimental group were cholesteroled with 2% high cholesterol regime and received drug solvent, sugar cane extract with 3.7 and 7.4 mg/kg doses as an oral treatment per day, respectively . After eight weeks of treatment, Blood were collected and subjected for measuring of LDL-C and HDL-c. For histological studies, Aorta was removed and was fixed in formalin %10. SPSS with ANOVA was applied for data analysis. Results: The results demonstrated treatment via sugar cane extract with 3.7 and 7.4 mg/kg per day, significant reduction in LDL-c and significant increase in HDL-c, in compare with Sham group, was occurred. In addition, histological examination showed that treatment by 3.7 and 7.4 mg/kg per day of Sugar cane extract (the experimental groups 1, 2, respectively), prevented atheroma plaques. Conclusion: Sugar cane extract may be effective in reducing the amount of LDL-c and increasing HDL-c and prevents the formation of atheroma plaques. http://jbrms.medilam.ac.ir/article-1-82-en.pdf 2015-02-22 1 6 Sugar cane LDL-c HDL-c atherosclerosis Mansour Amraei amraei_m@yahoo.com 1 Dept of Physiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran AUTHOR Safoura Mohamadpour safoura.mohamadpour@yahoo.com 2 Dept of Physiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran AUTHOR Mohamadhesam Amraei amraei_m@yahoo.com 3 Dept of Physiology, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran AUTHOR
OTHERS_CITABLE Follicular fluid the best medium of Maturation, fertilization and development of immature oocytes Introduction: In vitro maturation and development of immature oocytes, as an artificial reproduction technique, is useful especially in women who are affected by cancer and polycystic ovary syndrome. Despite using many types of in vitro media, an appropriate environment has not been reported yet. Present study was designed to assess the effect of heated human follicular fluid (hHFF), which is similar to in vivo environment for oocyte, on the maturation and fertilization potential of mouse immature oocytes. Materials and methods: Healthy female mice, aged 4-6 weeks, were sacrificed via cervical dislocation and their ovaries were extracted under sterile conditions. After washing, the separated immature oocytes were divided into three groups: In the first group, 236 immature oocytes were placed in culture medium contained DMEM, HCG, FSC 25%, and rFSH. In the second group, 229 immature oocytes were put in culture medium contained 100% hHFF. In the third group, 255 immature oocytes were placed in culture medium contained DMEM, HCG, rFSH, and 25% hHFF. Immature oocytes were placed in an incubator for 24 hours. Then, the stages of oocyte maturation were assessed by invert microscope and mature oocytes in each group were transferred to sperm-contained drops. After 24 hr, rate of two-cell embryos was recorded using invert microscope. Data was analyzed by Chi square test. Results: Maturation rate of oocytes in the second group (87.8%) was significantly higher than first (64.9%) and third (63.2%) groups (p<0.0005). The difference between first and third groups was not statistically significant (P<0.2). The formation rate of two-cell embryo in the second group (82.1%) was higher than first (50.2%) and third (54.3%) groups (p<0.002 and P<0.01, respectively). Conclusion: it seems hHFF could improve in vitro maturation and fertility potential of immature oocytes and consequently the formation rate of two-cell embryos in mice, in comparison with DMEM even supplemented with 25% hHFF. http://jbrms.medilam.ac.ir/article-1-77-en.pdf 2015-02-25 7 13 Follicular fluid Maturation fertilization oocytes Hojjat-Allah Abbaszadeh dr.abbaszadeh@yahoo.com 1 AUTHOR Reza Mastery Farahani dr.abbaszadeh@yahoo.com 2 AUTHOR Mohsen Noorozian dr.abbaszadeh@yahoo.com 3 AUTHOR Maryam Sadat Khoramgah dr.abbaszadeh@yahoo.com 4 AUTHOR Ardeshir Moayeri dr.abbaszadeh@yahoo.com 5 AUTHOR Ali Asghar Keramati Nia dr.abbaszadeh@yahoo.com 6 AUTHOR Mohammad Hassan Karimfar dr.abbaszadeh@yahoo.com 7 AUTHOR
OTHERS_CITABLE Job stress and susceptibility to cardiovascular diseases: A case-control study Introduction: Job stress is one of the main characteristics of modern life which is increasing and has been recognized as an important risk factor of cardiovascular diseases. The present study was conducted to determine the association between job stress at work environment and cardiovascular diseases. Materials and methods: A number of 300 persons including 150 cases and 150 controls were considered in the present study. The participants were diagnosed as patient or healthy persons, based on the cardiologist visits. Then, ERI and demographic questionnaires were completed by interview with subjects. After data collection, the data was interpreted, scored and analyzed using SPSS-16 (chi-square and independent sample T tests). Results: Mean age of subjects was 54.2±11.3 years. There was a significant difference between case and control groups in term of extrinsic effort and esteem reward (p=0.05). No significant difference was also observed between case and control groups in term of effort and reward imbalance (p=0.059). Mean fasting blood sugar and triglycerides in patients with effort-reward imbalance was higher than in patients with effort-reward balance (p=0.05). Conclusion: Cardiovascular disease has a close association with risk factors including job stress. Health education and screening of risk factors of cardiovascular diseases are recommended to early diagnosis of such diseases. http://jbrms.medilam.ac.ir/article-1-85-en.pdf 2015-02-27 14 20 Job stress cardiovascular disease case-control study Kobra Farokhimoghadam k_farokhimoghadam@yahoo.com 1 AUTHOR Yousef Mohsenzade k_farokhimoghadam@yahoo.com 2 AUTHOR Heshmatollah Nourmoradi k_farokhimoghadam@yahoo.com 3 AUTHOR Reza Beiranvand k_farokhimoghadam@yahoo.com 4 AUTHOR Kourosh Soleiman Nejad k_farokhimoghadam@yahoo.com 5 AUTHOR Ali Delpisheh alidelpisheh@yahoo.com 6 AUTHOR Sima Avish k_farokhimoghadam@yahoo.com 7 AUTHOR Tahereh Sahraei k_farokhimoghadam@yahoo.com 8 AUTHOR
OTHERS_CITABLE Environmental health and safety in elementary schools: A case study in Ilam city, Iran Introduction: Students in elementary schools due to the physical and behavioral characteristics more than adults have exposed to environmental hazards. This study was conducted to examine the environmental health and safety indices in girls' elementary schools in Ilam city. Materials and methods: This cross-sectional study was carried out via census sampling method in girls' elementary schools (21 schools) in Ilam city. Data were collected through a checklist of management system evaluation. Finally, the data were analyzed by SPSS-16 and the schools were classified in 5 levels (1 to 5 stars). Results: The results showed that 71.4 %, 66.7 % and 85.7 % of the schools were desirable in term of providing clinical services, improving nutrition in schools and parents and society participation in health promoting program, respectively. However, 100% of the schools in term of promoting the school staff were in undesirable condition. Conclusion: According to the results, most schools in term of environmental health and safety indices were in moderate condition. It was revealed that 33% of the schools, based on the health improvement program, were classified as 1 star and no school was found as 5 stars. This issue requires more attention to improve and promote the schools health level by related authorities. http://jbrms.medilam.ac.ir/article-1-81-en.pdf 2015-02-27 21 27 Elementary School Environmental Health Safety Feizollah Mirzaei feam.1391@gmail.com 1 Ilam University of Medical Sciences AUTHOR Fatemeh Nori fatemehnori123@gmail.com 2 Ilam University of Medical Sciences AUTHOR Mehdi Mirzaei raeis68@yahoo.com 3 Zanjan University of Medical Sciences AUTHOR Heshmatollah Nourmoradi Ilam_nourmoradi@yahoo.com. 4 Ilam University of Medical Sciences AUTHOR
OTHERS_CITABLE Evaluation of Ilam university of medical science faculty members\' views in relation to curriculum Introduction: Curriculum has a major impact on education. The faculty members are the most important element in the success of the curriculum. The aim of this study was to determine the viewpoints of university of medical science faculty in relation to factors affecting their participation in the academic curricula. Materials and methods: This was a cross-sectional study which included 66 members university of medical science faculty, from Ilam-IR, during the year 2014. Data collection tool was a questionnaire and sampling method was census. Results: According to findings, scientific (27.4±9.8) and outside the organization factors (9.1±2.8) on their participation in the curriculum was moderate . The impact of Professional factors (28.4±7.2), internal organizational factors (25.7±6.1) , internal (19.1±4.5) and external motivational factors (19.1±4.5) on members' participation in curriculum planning was the high level. Motivational factors most impact on the participation of faculty in academic curricula. Conclusion: Organization and educational managers should use programs that can improve job satisfaction, organizational commitment, responsibility and self-confidence, improving scientific, academic freedom and professional autonomy, attention to the law, the facilities and faculty teamwork culture, workshops and conferences to increase their participation in academic curricula recommended. http://jbrms.medilam.ac.ir/article-1-83-en.pdf 2015-03-03 28 33 Curriculum Faculty member Education Medical Science Molouk Jaafarpour jaafarpourm@gmail.com 1 Department of Midwifery, Nursing & Midwifery Faculty, Ilam University of Medical Science, Ilam, IR-Iran AUTHOR Meysam Gholami ghm_msm@yahoo.com 2 Student Research Committee,Ilam University of Medical Sciences, Ilam, Iran. AUTHOR Ali Akbar Jafarpour ajafarpour@gmail.com 3 Department of Educational systems, School of Agriculture, Kermanshah, IR- Iran. AUTHOR Ali Khani nimakhani@gmail.com 4 Department of Nursing, Nursing & Midwifery Faculty, Ilam University of Medical Science, Ilam, IR-Iran. AUTHOR
OTHERS_CITABLE Using transient transfected Chinese hamster ovary (CHO) cells by pET28a-LIC-NTF4 in gene therapy Introduction: Neurotrophins, as a family of proteins, are responsible for induction of the survival, development, and function of neurons. Also, neurotropic factors are growth factors like Neurotrophins that help neurons survive. Moreover, Neurotrophins differentiate between progenitor cells so that neurons are formed. Despite the fact that the majority of mammalian brain neurons are produced prenatally, the capability of growing new neurons from neural stem cells will be preserved by parts of the adult brain. This process is known as neurogenesis. Neurogenesis is stimulated and controlled by neurotrophins. Materials and methods: The recombinant plasmid transformed to E.coli ยด cell and colonies that contain plasmid were selected by Colony PCR. Enzyme digestion and sequencing were monitored to approve the accuracy of extracted plasmid of the clones. Results: Plasmid was verified correctly. Based on RT-PCR and western blotting, the transcription of NTF4 gene and the expression of NTF4 protein after transfection were proved. Conclusion: Plasmid was correctly constructed, CHO Cells were successfully transfected by transfection, and protein could be properly expressed. The results provided a solid foundation for the studies in the area of the transplantation of gene-modified CHO Cells to further spinal cord regeneration. http://jbrms.medilam.ac.ir/article-1-87-en.pdf 2015-03-07 34 41 Transfection Cloning Molecular Neurotrophins 4 Genetic Vectors Mohammad Hassan Karimfar 1 AUTHOR Reza Mastery Farahani 2 AUTHOR Mohsen Noorozian 3 AUTHOR Maryam Sadat Khoramgah 4 AUTHOR Ali Asghar Keramati Nia 5 AUTHOR Ardeshir Moayeri 6 AUTHOR Hadi Azimi 7 AUTHOR Hojjat-Allah Abbaszadeh 8 AUTHOR
OTHERS_CITABLE Induction of type 2 diabetes with high concentration and long term fructose intake in male Sprague-Dawley rats Introduction: High calorie diet is becoming the most important health problem in most developed western societies. Diets with high caloric contents such as high fructose intake are associated with increasing risk of type two diabetes (T2D) and insulin resistance (IR). However, large differences have been seen in the dose and duration of fructose consumption to induction of T2D. Interest to develop animal models, in which IR is induced by feeding a high caloric diet, is the main aim of this study. Materials and methods: Male Sprague-Dawley rats were randomly sorted into three groups, control and fructose-treated (10 and 20 %) animals. After 6 and 14 weeks, fluid intake, body weight, intraperitoneal glucose tolerance test, metabolic parameters (glucose, insulin, triglyceride, cholesterol, uric acid) and homeostasis model assessment of insulin resistance were checked. Results: There was no difference between the body weight of the fructose-treated (10 and 20 %) and control groups during the experiment. Fluid intake in fructose-treated ones (10 and 20 %) was significantly higher than the control group from third week to the end of experiment. Intraperitoneal glucose tolerance test was the same for all groups in either sixth or fourteenth weeks. Fasting plasma insulin, triglyceride, uric acid and homeostasis model assessment of insulin resistance and glucose tests showed significant enhancement in fructose-treated rats (20%) only after 14 weeks. Conclusion: Consumption of high fructose solution (20%) for 14 weeks could induce IR, but using of low dose of fructose (10% for 6 or 14 weeks) didn’t develop it. http://jbrms.medilam.ac.ir/article-1-80-en.pdf 2015-03-10 42 50 Fructose Insulin resistance Type two diabetes Sprague-Dawley rat Enaiat Anvari Anvari_ph@yahoo.com 1 Department of physiology, Shiraz University of Medical Sciences, Shiraz, Iran AUTHOR Sara Keshtgar Anvari_ph@yahoo.com 2 AUTHOR Ali Noorafshan Anvari_ph@yahoo.com 3 AUTHOR Ali Rafati A_rafati@yahoo.com 4 AUTHOR
OTHERS_CITABLE Prevalence of viral infection of blood donors in Ilam blood transfusion center Introduction:Blood-borne infectious agents are one of the main anxieties of Blood Transfusion Organization in all countries. According to WHO, the blood healthy and blood safety is included ensuring and monitoring of quality and efficiency for all patients requiring blood transfusion. We at the current study intend that survey prevalence HIV Ab, HCV Ab, HBS Ag Among blood donors refer to the Blood Transfusion Organization of Ilam in 2010-2011. Materials and methods:The sample size was folders of 4005 subjects who entered into the study with census method. Screening test was performed for hepatitis B surface antigen, Antibodies against hepatitis C virus and HIV Ab. For confirmed positive result that obtain by screening test we use for HBV, HCV, HIV respectively. Results: Number (percent) of subjects with hepatitis B (HBV), hepatitis C (HCV) and HIV among the infected blood donors were 29 (70.7%), 11 (26.8%) and 1 (2.5%), respectively. There was no significant association between education level and disease (P>0.05), Significant correlation (P<0.01) was found between job variables and disease Conclusion: The low prevalence of HIV infection in this study and other similar studies in Iran May be due to the higher percentage of more regular volunteer donors in Iran As the World Health Organization studies show that voluntary donors are with the lowest prevalence of HIV, hepatitis viruses and other blood-borne infections http://jbrms.medilam.ac.ir/article-1-90-en.pdf 2015-03-13 51 55 Prevalence Viral Infection Blood Donors Farajolah Maleki fmaleki88@yahoo.com 1 Ilam University of Medical Sciences AUTHOR Saeid Hemati fmaleki88@yahoo.com 2 Ilam University of Medical Sciences AUTHOR Zahra Mahdavi amraei_m@yahoo.com 3 AUTHOR Mitra Azizian fmaleki88@yahoo.com 4 Ilam University of Medical Sciences AUTHOR Mansour Amraei amraei_m@yahoo.com 5 Ilam University of Medical Sciences AUTHOR