Successful treatment of thrombotic thrombocytopenic purpura during pregnancy: A case report 2 2 A 28 years’ pregnant woman with 24 weeks’ gestational age referred with petechiae and purpura from previous day without any trauma. She had an occipital headache from last night. Overt petechial and purpuric lesions were seen in the mouth and skin. There was neither hepatosplenomegaly nor lymphadenopathy. She was conscious and oriented. The patient was febrile, anemic and thrombocytopenic with stable vital signs. All liver enzymes were elevated. Coagulation profile was normal. WBCs were normal. RBCs were reduced, and she had polychromatosis. Overt shistocytosis was seen. Platelets were significantly decreased. The first diagnosis was TTP. All necessary laboratory tests were done to rule out the secondary rheumatologic causes of TTP; which all were normal. Coombs tests were negative. ADAMTS 13 Ab was elevated. Fetal ultrasonography was normal. Treatment started with plasmapheresis and corticosteroid. After treatment, platelets count begins elevated, and LDH decreased. The patient discharged with a good general condition and normal lab tests. She continued her pregnancy until term, and born a normal infant without any complication. She did not have a recurrence of TTP until September 2014. 1 3 2016/02/28 1394/12/9 2016/08/14 1395/5/24 Elham Naghshineh Elham Naghshineh Department of Obstetrics/Gynecology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Iran naghshineh@med.mui.ac.ir Mehrdad Mostaghaci Mehrdad Mostaghaci Department of Occupational Medicine, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran Iran mehrdadmostaghaci@gmail.com TTP Pregnancy Treatment Scully M, Thomas M, Underwood M, Watson H, Langley K, Camilleri RS, et al. Thrombotic thrombocytopenic purpura and pregnancy: presentation, management, and subsequent pregnancy outcomes. Blood. 2014; 124(2):211-9.## Ducloy-Bouthors AS, Caron C, Subtil D, Provot F, Tournoys A, Wibau B, et al. Thrombotic thrombocytopenic purpura: medical and biological monitoring of six pregnancies. Eur J Obstet Gynecol Reprod Biol. 2003;111(2):146-52.## George JN. The association of pregnancy with thrombotic thrombocytopenic purpura–hemolytic uremic syndrome. Curr Opin Hematol. 2003;10(5):339-44.##Uğur Bilgin A, Karaselek MA, Çamlı K. Successful management of thrombotic thrombocytopenic purpura associated with pregnancy. Transfus Apher Sci. 2014;50(3):433-7.## Castella M, Pujol M, Julia A, Massague I, Bueno J, Ramon Grifols J, et al. Thrombotic thrombocytopenic purpura and pregnancy: a review of ten cases. Vox Sang. 2004;87(4):287-90.##Allford S, Hunt B, Rose P, Machin S. Haemostasis and thrombosis task force, British committee for standards in haematology. Guidelines on the diagnosis and management of the thrombotic microangiopathic haemolytic anaemias. Br J Haematol. 2003;120(4):556-73.##McMinn JR, George JN. Evaluation of women with clinically suspected thrombotic thrombocytopenic purpura‐hemolytic uremic syndrome during pregnancy. J Clin Apher. 2001;16(4):202-9.## Mwita JC, Vento S, Benti T. Thrombotic Thrombocytopenic Purpura-Haemolytic Uremic Syndrome and pregnancy. Pan Afr Med J. 2014; 17:255. ## ##

Prevalence of HIV, hepatitis B and C infections among volunteer blood donors at the blood transfusion center of Ilam city, Iran 2 2 Introduction: Blood derived products have been known as an effective treatment for many years. However, this treatment is not without risk of infections transmission including hepatitis B virus (HBV) and hepatitis C virus (HCV) and human immune-deficiency virus (HIV) in people who received the blood. Nevertheless, due to a high risk of blood born diseases through blood transfusion, screening for these viruses according to the World Health Organization (WHO) is mandatory. The main aim of this research was to assess the prevalence of HBV, HCV and HIV infections among healthy blood donors of Blood Donor Center of Ilam (BDCI). Materials and methods: In this study we used the information from first and repeated blood donors who referred to BDCI within February 2009 to January 2013. Demographic characteristics of donors including marital status, age, gender and blood donation pattern was extracted. Routine donor laboratory screening tests for HBV, HIV and HCV were performed. Results: HBV infection had the highest prevalence (0.14%) while HIV had the lowest ones (0.006%). The highest prevalence was among male blood donors. The prevalence of HBv, HIV and HCV infections were more common among men and first time donors (P < 0.01). The prevalence of HIV and HCV infections were more common among married donors than singe ones (P < 0.01) HBV prevalence in singles was more (P < 0.05) compared to married blood donors. The highest and the lowest subjects with HBV, HCV and HIV infections were in range of 51 to 60 years and 18-35 years old, respectively. Conclusion: It is estimated that the prevalence of HBV, HCV and HIV infections are low in voluntary blood donors than general population which confirmed the effectiveness of education and examination of blood donors. This usually arising from the pre donation screening for risky behaviors, so deleting the high risk people. Since unsafe blood products are not used for blood transfusion, they are not considered as risk for blood safety system, but identification of these blood units is a problem for blood transfusion centers. 4 8 2016/02/282016/04/14 1395/1/26 2016/08/142016/07/2 1395/4/12 Hasan Boustani Hasan Boustani Department of Hematology, Faculty of Allied medical Sciences, Ilam University of Medical Sciences, Ilam, Iran Iran anvari_ph@yahoo.com Enayat Anvari Enayat Anvari Student’s Research Committee, Ilam University of Medical Sciences, Ilam, Iran Iran anvari_ph@yahoo.com Sedighe Saiadi Sartang Sedighe Saiadi Sartang Student’s Research Committee, Ilam University of Medical Sciences, Ilam, Iran Iran anvari_ph@yahoo.com Mehdi Omidi Mehdi Omidi Department of Mathemetics, Ilam University, Ilam, Iran Iran omidi_280@yahoo.com Esmaeil Rostami Esmaeil Rostami Department of Hematology, Faculty of Allied medical Sciences, Sabzevar University of Medical Sciences, Sabzevar, Iran Iran hasanboostani@yamail.com Zahra Mohamadi Zahra Mohamadi Student’s Research Committee, Ilam University of Medical Sciences, Ilam, Iran Iran hasanboostani@yamail.com Blood donor Ilam City Hepatitis B virus Hepatitis C virus HIV Schreiber GB, Busch MP, Kleinman SH, Korelitz JJ. The risk of transfusion transmitted viral infections. N Engl J Med. 1996; 334(26):1685-90.## Sheikh MY, Atla PR, Ameer A, Sadiq H, Sadler PC. Seroprevalence of Hepatitis B and C Infections among Healthy Volunteer Blood Donors in the Central California Valley. Gut Liver. 2013; 7(1): 66–73.## Munro JB, Mothes W. Structure and dynamics of the native HIV-1 Env trimer. Virology. 2015; 89 (11): 5752-5.##World Health Organization. Screening Donated Blood for Transfusion Transmissible Infections Recommendations. 2009; ISBN: 9789241547888.## Majdzadeh R, Pouajal J. Prevalnce of Hepatitis B in Iran, a systematic review. Epidemiology. 2008; 4 (3): 1-8. ## Glynn SA, Kleinman SH, Schreiber GB, Busch MP, Wright DJ, Smith JW, et al. Trends in incidence and prevalence of major transfusion-transmissible viral infections in US blood donors, 1991 to 1996. Retrovirus Epidemiology Donor Study (REDS). JAMA. 2000;284(2):229-35.## Busch MP, Young MJ, Samson SM, Mosley JW, Ward JW, Perkins HA. Risk of human immunodeficiency virus (HIV) transmission by blood transfusions before the implementation of HIV-1 anti‌body screening. The Transfusion Safety Study Group. Transfusion. 1991;31(1):4-11.##Zuck TF. Transfusion-transmitted AIDS reassessed. N Engl J Med. 1988;318(8):511-2.## Bastiaans MJ, Nath N, Dodd RY, Barker LF. Hepatitis-associated markers in the American Red Cross volunteer blood donor popu‌lation. IV. A comparison of HBV-associated serologic markers in HBsAg-positive first-time and repeat blood donors. Vox Sang. 1982;42(4):203-10.## Murphy EL, Fang J, Tu Y, Cable R, Hillyer CD, Sacher R, et al. Hepatitis C virus prevalence and clearance among US blood donors, 2006-2007: associations with birth cohort, multiple pregnancies, and body mass index. J Infect Dis. 2010;202(4):576-84.##Regev A, Schiff ER. Viral hepatitis A, B, and C. Clin Liver Dis. 2000;4(1):47-71, vi. Review.## Khodabandehloo M, Roshani D, Sayehmiri K. Prevalence and trend of hepatitis C virus infection among blood donors in Iran: A systematic review and meta-analysis. J Res Med Sci. 2013;18(8):674-82.## Kafi-abad SA, Rezvan H, Abolghasemi H, Talebian A. Prevalence and trends of human immunodeficiency virus, hepatitis B virus, and hepatitis C virus among blood donors in Iran, 2004 through 2007. Transfusion. 2009;49(10):2214-20.## Zali MR, Adibi P, Alavian SM. Hepatitis C virus in Iran: epidemiology of an emerging infection. Arch Iranian Med. 2005; 8 (2): 84-90.## Persian BBC. 14 December 2015.## ##

The short-term survival following acute myocardial infarction: A prospective observational study 2 2 Introduction: To investigate 28 days survival rate following first acute myocardial infarction (AMI) associated with the presence of classical risk factors and treatment modalities in Gorgan, north of Iran. Materials and methods: Our cohort including all patients hospitalized due to AMI from 2010 to 2013. Data were collected on demographic, prophylactic drugs and classical coronary risk factors. Data were analyzed using Kaplan-Meier and log rank tests. Cox proportional hazard model was built to estimate relative risk by taking into account other variables. Results: Median age of subjects was 58 and 64 for men and women, respectively; moreover, the occurrence of diabetes was 52.5 and 24.5 for them. A poorer outcome was detected in survival rate for women in the final model. Excess death occurrence in Fars or non- Persian ethnicity, and those who were not prescribed statin was detected in the adjusted model. Conclusion: A better survival rate for those who were prescribed statin suggest that it could be beneficial in treatment modality and non-Persian ethnicities needed to be considered as a group of at risk for earlier screening programs. In spite of some other studies a poorer outcome following AMI for women was found even after taking into account age and comorbidity. 9 16 2016/02/282016/04/142016/01/31 1394/11/11 2016/08/142016/07/22016/07/15 1395/4/25 Mohammad Aryaie Mohammad Aryaie Health Management and Social Development Research Center, Golestan University of Medical Sciences, Gorgan, Iran Iran aryaie@goums.ac.ir Fozieh Bakhsha Fozieh Bakhsha Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran Iran bakhsha_fo@yahoo.com Seyyed Yaghub Jafari Seyyed Yaghub Jafari Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran Iran syjafarib@gmail.com Zahra Yousefi Zahra Yousefi Laboratory Sciences Research Center, Golestan University of Medical Sciences, Gorgan, Iran Iran stu.yousefi@yahoo.com Ali Reza Heidari Ali Reza Heidari Health Management and Social Development Research Center, Golestan University of Medical Sciences, Gorgan, Iran Iran Heidari_ar_2000@yahoo.com Zahra Esmaeili Zahra Esmaeili Research Committee, Golestan University of Medical Science, Gorgan, Iran Iran zahraa.esmaeily@yahoo.com Sakineh Sobhani Sakineh Sobhani Research Committee, Golestan University of Medical Science, Gorgan, Iran Iran Yassobhani28@gmail.com Shima Aghili Shima Aghili Research Committee, Golestan University of Medical Science, Gorgan, Iran Iran sh_aghili92@yahoo.com Acute myocardial infarction Survival Risk factor Treatment Kim AS, Johnston SC. Global variation in the relative burden of stroke and ischemic heart disease. Circulation. 2011;124(3):314-23.##Griffith D, Hamilton K, Norrie J, Isles C. Early and late mortality after myocardial infarction in men and women: prospective observational study. Heart. 2005;91(3):305-7.##McGovern PG, Jacobs DR, Shahar E, Arnett DK, Folsom AR, Blackburn H, et al. Trends in acute coronary heart disease mortality, morbidity, and medical care from 1985 through 1997 the Minnesota Heart Survey. Circulation. 2001; 104(1):19-24.##Tunstall-Pedoe H, Kuulasmaa K, Mahonen M, Tolonen H, Ruokokoski E. Contribution of trends in survival and coronar y-event rates to changes in coronary heart disease mortality: 10-year results from 37 WHO MONICA Project populations. Lancet. 1999;353(9164):1547-57.## Naghavi M, Abolhassani F, Pourmalek F, Lakeh MM, Jafari N, Vaseghi S, et al. The burden of disease and injury in Iran 2003. Popul Health Metr. 2009;7(1):9.## Kubota I, Ito H, Yokoyama K, Yasumura S, Tomoike H. Early mortality after acute myocardial infarction: observational study in Yamagata, 1993-1995. Jpn Circ J. 1998;62(6):414.## Davies CA, Leyland AH. Trends and inequalities in short-term acute myocardial infarction case fatality in Scotland, 1988-2004. Popul Health Metr. 2010; 8(33):1-8.##Schroder R, Biamino G, Von Leitner ER, Linderer T, Bruggemann T, Heitz J, et al. Intravenous short-term infusion of streptokinase in acute myocardial infarction. Circulation. 1983;67(3):536-48.##Wilcox RG, Olsson CG, Skene AM, Von Der Lippe G, Jensen G, Hampton JR, et al. Trial of tissue plasminogen activator for mortality reduction in acute myocardial infarction: Anglo-Scandinavian Study of Early Thrombolysis (ASSET). Lancet. 1988;332(8610):525-30.## Abildstrom SZ, Rasmussen S, Rosen M, Madsen M. Trends in incidence and case fatality rates of acute myocardial infarction in Denmark and Sweden. Heart. 2003;89(5):507-11.##Rosen M, Alfredsson L, Hammar N, Kahan T, Spetz C-L, Ysberg A-S. Attack rate, mortality and case fatality for acute myocardial infarction in Sweden during 1987-95. Results from the national AMI register in Sweden. J Intern Med. 2000;248(2):159-64.## Yusuf S, Zucker D, Passamani E, Peduzzi P, Takaro T, Fisher LD, et al. Effect of coronary artery bypass graft surgery on survival: overview of 10-year results from randomised trials by the Coronary Artery Bypass Graft Surgery Trialists Collaboration. Lancet. 1994;344(8922):563-70.## Yusuf S, Peto R, Lewis J, Collins R, Sleight P. Beta blockade during and after myocardial infarction: an overview of the randomized trials. Prog Cardiovasc Dis. 1985;27(5):335-71.## Hampton JR. Coronary artery bypass grafting for the reduction of mortality: an analysis of the trials. Br Med J (Clinical research ed). 1984;289(6453):1166.## Maynard C, Every NR, Martin JS, Kudenchuk PJ, Weaver WD. Association of gender and survival in patients with acute myocardial infarction. Arch Intern Med. 1997;157(12):1379.## Tunstall-Pedoe H, World Health O. WHO MONICA project. Geneva: WHO. 2003.## Tunstall-Pedoe H, Kuulasmaa K, Amouyel P, Arveiler D, Rajakangas A-M, Pajak A. Myocardial infarction and coronary deaths in the World Health Organization MONICA Project. Registration procedures, event rates, and case-fatality rates in 38 populations from 21 countries in four continents. Circulation. 1994;90(1):583-612.## Gottlieb S, Harpaz D, Shotan A, Boyko V, Leor J, Cohen M, et al. Sex Differences in Management and Outcome After Acute Myocardial Infarction in the 1990s A Prospective Observational Community-Based Study. Circulation. 2000;102(20):2484-90.## Hafshejani A, Lari M, Hafshejani F. Predicting Factors of Short-term Survival in Patients with Acute Myocardial Infarction in Isfahan Using a Cox Regression Model. Iran J Epid. 2012;8(2)## Koek HL, de Bruin A, Gast F, Gevers E, Kardaun JWPF, Reitsma JB, et al. Short-and long-term prognosis after acute myocardial infarction in men versus women. Am J Cardiol. 2006;98(8):993-9.## MacIntyre K, Stewart S, Capewell S, Chalmers JWT, Pell JP, Boyd J, et al. Gender and survival: a population-based study of 201,114 men and women following a first acute myocardial infarction. J Am Coll Cardiol. 2001;38(3):729-35.##Tunstall-Pedoe H, Morrison C, Woodward M, Fitzpatrick B, Watt G. Sex differences in myocardial infarction and coronary deaths in the Scottish MONICA population of Glasgow 1985 to 1991 Presentation, diagnosis, treatment, and 28-day case fatality of 3991 events in men and 1551 events in women. Circulation. 1996;93(11):1981-92.##Herman B, Greiser E, Pohiabeln H. A sex difference in short-term survival after initial acute myocardial infarction The MONICA-Bremen Acute Myocardial Infarction Register, 1985-1990. Eur Heart J. 1997;18(6):963-70.## Kudenchuk PJ, Maynard C, Martin JS, Wirkus M, Weaver WD, Investigators MP. Comparison of presentation, treatment, and outcome of acute myocardial infarction in men versus women (the Myocardial Infarction Triage and Intervention Registry). Am J Cardiol. 1996;78(1):9-14.## Anderson RT. Quality of care among disadvantaged women: adding income level to health datasets.WHI. 2003;13(5):177-9.## Koh HK, Judge CM, Ferrer B, Gershman ST. Using public health data systems to understand and eliminate cancer disparities. Cancer Causes Control. 2005;16(1):15-26.##Machin Mn, Aldasoro E, Martأnez-Camblor P, Calvo M, Basterretxea M, Audicana C, et al. Socioeconomic differences in incidence and relative survival after a first acute myocardial infarction in the Basque Country, Spain. Gaceta Sanitaria. 2012;26(1):16-23.## Picciotto S, Forastiere F, Stafoggia M, D'Ippoliti D, Ancona C, Perucci CA. Associations of area based deprivation status and individual educational attainment with incidence, treatment, and prognosis of first coronary event in Rome, Italy. 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Comparison of short-and long-term prognosis in patients with anterior wall versus inferior or lateral wall non-Q-wave acute myocardial infarction. Am J Cardiol. 1997;79(6):717-21.## ##

Functional recovery assessment of spinal cord contusion model in male rats without therapeutic interventions 2 2 Introduction: Spinal cord injury (SCI) is one of the most serious clinical diseases, which not only affects the patient's physical and mental status, but its effects will be spread to family and community. After severe spinal cord injury, astrocytes of the central nervous system (CNS) become reactive astrocytes, and play the main role of glial scar formation. The scar is a major obstacle to regeneration of axons in the spinal cord. However, the studies have found that over time, a spontaneous partial motor recovery is observed in animals with injury without intervention. Thus, in this study, the recovery of animals with spinal cord injury was assessed after 12 weeks. Materials and methods: In this study, 12 adult male Wistar rats weighing approximately 265±15gr were used to assess spinal cord injury and randomly divided into 3 groups: normal control (n = 3), sham (n = 3), injury (n = 6). Healthy animals in the normal control group received no laminectomy or injury, and laminectomy with or without contusion model using weight drop in segment T10 of spinal cord were carried out in injury and sham groups, respectively. Locomotor function of animals in all groups were evaluated by BBB test at the first 48 hours per day and then weekly for 12 weeks. Results: Comparison of the results of motor evaluation from the second week to the twelfth week of the group with injury without treatment showed a relative functional recovery as the BBB score of animals from 1.4 in the second week after the injury reached to 6.5 in the twelfth week. Conclusion: The results indicate a spontaneous partial recovery in injured animals without intervention. 17 23 2016/02/282016/04/142016/01/312016/03/25 1395/1/6 2016/08/142016/07/22016/07/152016/08/8 1395/5/18 Sara Rezaei Sara Rezaei Department of Anatomy, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran Iran azizi.moaz@gmail.com Salar Bakhtiari Salar Bakhtiari Department of Biochemistry, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran Iran rezaei7194@yahoo.com Khairollah Asadollahi Khairollah Asadollahi Department of Epidemiology, Faculty of Health, Ilam University of Medical Sciences, Ilam, Iran Iran bakhtiyaribio@gmail.com Somayeh Heidarizadi Somayeh Heidarizadi Department of Anatomy, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran Iran masoud_1241@yahoo.co.uk Ardeshir Moayeri Ardeshir Moayeri Department of Anatomy, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran Iran somaye 6001@yahoo.com Monireh Azizi Monireh Azizi Department of Anatomy, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran Iran moayeri46@yahoo.com Spinal cord injury Glial scar Functional recovery Rat Beattie MS, Hermann GE, Rogers RC, Bresnahan JC. Cell death in models of spinal cord injury. Prog Brain Res. 2002; 137: 37-47.## Sie I, Waters R. Outcomes following spinal cord injury. In: Lin V, editor. Spinal cord medicine: principles and practice. New York: Demos Medical Publishing; 2003. p. 87-103.## Okada S, Nakamura M, Katoh H, Miyao T, Shimazaki T, Ishii K, et al. Conditional ablation of State3 or Socs3 discloses a dual role for reactive astrocytes after spinal cord injury. Nat Med. 2006; 12(7): 829-34.## Kundi S, Bicknell R, Ahmed Z. Spinal cord injury: Current mammalian models. Am J Neurosci. 2013; 4 (1):1-12.## Young W. Spinal cord contusion models. Prog Brain Res. 2002; 137:231-255.## Tator CH. Update on the pathophysiology and pathology of acute spinal cord injury. Brain Pathol.1995; 5(4): 407-13.## Kostyk S, Popovich P, Stokes B. Mechanisms and natural history of spinal cord injury. In: Lin V, editor. Spinal cord medicine: principles and practice. New York: Demos Medical Publishing; 2003. p. 765-76.## Oudega M, Moon LD, de Almeida Leme RJ. Schwann cells for spinal cord repair. Braz J Med Biol Res. 2005; 38(6):825-35.## Moreno-Manzano V, Rodriguez-Jimenez F, Garcia-Rosello M, Lainez S, Erceg S, Calvo M, et al. Activated spinal cord ependymal stem cells rescue neurological function. Stem Cell. 2009; 27(3):733-43.##Mothe AJ, Tator CH. Proliferation, migration, and differentiation of endogenous ependymal region stem/ progenitor cells following minimal spinal cord injury in the adult Rat. Neuroscience. 2005; 131(1): 177-87.## McDonough A, Martinez-Cerdeno V. Endogenous proliferation after spinal cord injury in animal models. Stem Cells Int. 2012; 2012:387513.## Filbin MT. Axon regeneration, vaccinating against spinal cord injury. Curr Biol. 2000; 10(3): R100-R103.## De Winter F, Oudega M, Lankhorst AJ, Hamers FP, Blits B, Ruitenberg MJ, et al. Injury –induced class 3 semaphorin expression in the rat spinal cord. Exp Neurol. 2002; 175(1): 61-75.## Properzi F, Carulli D, Asher RA, Muir E, Camargo LM, Kuppevelt THV, et al. Chondroitin 6-sulphate synthesis is up-regulated in injured CNS, induced by injury related cytokines and enhanced in axon-growth inhibitory glia. Eur J Neurosci. 2005; 21(2):378-90.## Yaojian R, Wenxiao Z, Huijuan L,Chunxia S, Qingan Z, Yongjun W, et al. Clinical application of olfactory ensheathing cells in the treatment of spinal cord injury. J Int Med Res. 2013; 41(2): 473-81.##Fawcett JW, Asher RA. The glial scar and central nervous system repair. Brain Res Bull. 1999; 49(6): 377–91.## Ueno M, Yamashita T. Strategies for regenerating injured axons after spinal cord injury – insights from brain development. Tar Ther. 2008; 2(2):253-64.## Verdu E, Garcia – Alias G, Joaquimfore S, Lopez –Vales R, Navarro X. Olfactory ensheating cells transplanted in lesion spinal cord prevent loss of spinal cord parenchyma and promote functional recovery. Glia. 2003; 42(3): 275-86.##Garcia –Alias G, Lopez-Vales R, Fores J, Navarro X, Verdue E. Acute transplantation of olfactory ensheating cells or schwann cells promotes recovery after spinal cord injury in the rat. J Neurosci Res. 2004; 75(5):632-41.## Richter MW, Fletcher PA, Liu J, Tetzlaff W, Roskams AJ. Lamina propria and olfactory bulb ensheathing cells exhibit differential integration and migration and promote differential axon sprouting in the lesioned spinal cord. Neuroscience. 2005; 25(46):10700-11.## Basso DM, Beattie MS, Bresnahan JC. Graded histological locomotor outcomes after spinal cord contusion using the NYU weight-drop device versus transaction. Exp Neurol.1996; 139(2):244-56.## Wang C, Chen J, Wu Y, Tsai M, Shyue S, Yang C , et al. Reduction in antioxidant enzyme expression and sustained inflammation enhance tissue damage in the subacute phase of spinal cord contusive injury. J Biomed Sci. 2011; 18(1): 13.##Fouad K, Schnell L, Bunge MB, Schweb ME, Liebscher T, Pearse DD. Combining schwann cell bridges and olfactory ensheathing glia grafts with chondroitinase promotes locomotor recovery after complete transection of the spinal cord. Neuroscience. 2005; 25(5):1169-78.## ##

The effect of lansoprazole administration during pregnancy on the placenta 2 2 Introduction: Lansoprazole is one of the proton pump inhibitor drugs widely used in the treatment of gastro-duodenal ulcers and disorders. However, there is not enough data about unexplored adverse effects of lansoprazole on the integrity of the placental barrier. Therefore, the present study was conducted to determine whether placental structure could be affected by lansoprazole administration. Materials and methods: A total of 24 pregnant Balb/C mice were randomly divided into one control and three experiment groups (n=6).The experimental animals were given 25, 50 and 100 mg/kg of lansoprazole intraperitoneally on days 6-16 of pregnancy. At the end point and on the day 17 of gestation all animals were sacrificed. Then, the placentas specimens were taken and processed for histological examinations. Histological sections were stained with hematoxylin-eosin and were examined under light microscopy. Results: The histological examinations showed remarkable cellular changes in the placenta after treatment with lansoprazole. The placentas from drug administrated mice exhibited conspicuous decrease in the spongy layers size when compared with controls. Also, both polymorph and mononuclear cell infiltration into placental parenchyma were seen in the animals treated with 100 mg/kg lansoprazole. In addition, dilation of the intervillous space, massive vasculature congestion, increased giant cell population and fibroblastic proliferation were seen in the placental tissues from experimental groups. Conclusion: The findings of the present study led us to investigate the effect of the lansoprazole administration on the mouse placenta. Taken together, this drug should not be prescribed during pregnancy. 24 28 2016/02/282016/04/142016/01/312016/03/252016/05/1 1395/2/12 2016/08/142016/07/22016/07/152016/08/82016/08/17 1395/5/27 Ali Louei Monfared Ali Louei Monfared Department of Basic Sciences, Faculty of Veterinary Medicine, University of Ilam, Ilam, Iran Iran alm722@gmail.com Morteza Shamsi Morteza Shamsi Department of Parasitology, Faculty of Medicine, Ilam of University of Medical Sciences, Ilam, Iran Iran shamsi_ilam@yahoo.com Histology Placenta Mouse Lansoprazole Health Kounis NG. Serum tryptase levels and the Kounis syndrome. Int J Cardiol. 2007; 114(5):407–8. ## Rosen R, Hu L, Amirault J, Khatwa U, Ward DV, Onderdonk A. 16S community profiling identifies proton pump inhibitor related differences in gastric, lung, and oropharyngeal microflora. J Pediatr. 2015; 166(1):917-23.## Vlahos NP, Vavilis GK, Giannelou AG, Georgopoulou CN, Kommata VJ, Kougias CT, Tsartsalis DN, Kounis GN, Mazarakis A, Batsolaki M, Gouvelou-Deligianni GV, Hahalis G, Kounis NG. Hypersensitivity to proton pump inhibitors: lansoprazole-induced Kounis syndrome. Int J Cardiol. 2009; 29(1): 94-6.## Kedika RR, Souza RF, Spechler SJ. Potential anti-inflammatory effects of proton pump inhibitors: a review and discussion of the clinical implications. Dig Dis Sci. 2009; 54(3):2312-7. ##Praga M, Sevillano A, Aunon P, Gonzalez E. Changes in the etiology, clinical presentation, and management of acute interstitial nephritis, an increasingly common cause of acute kidney injury. Nephrol Dial Transplant. 2014; 30(3):1472-9.## Lebwohl B, Spechler SJ, Wang TC, Green PH, Ludvigsson JF. Use of proton pump inhibitors and subsequent risk of celiac disease. Dig Liver Dis. 2014; 46(1):36-40.##Kahrilas PJ. Gastro esophageal reflux disease. N Engl J Med. 2008;359(5):1700–7.## Schardein JL, Furuhashi T, Ooshima Y. Reproductive and developmental toxicity studies of lansoprazole (AG-1749) in rats and rabbits. Jpn Pharmacol Ther. 1990; 18(1): 119–29.## Pasternak B, Hviid A. Use of proton-pump inhibitors in early pregnancy and the risk of birth defects. N Engl J Med. 2010; 363 (22): 2114-23.## Sooranna SR, Oteng-ntim E, Meah R, Ryder TA, Bajoria R. Characterization of human placental explants: morphological, biochemical and physiological studies using first and third trimester placenta. Hum Reprod. 1999; 14(2): 536–41.## Briggs GG, Freeman RK, Yaffe SJ., editors. Drugs in pregnancy and lactation: a reference guide to fetal and neonatal risk. 9th ed. Philadelphia: Wolters Kluwer/ Williams & Wilkins; 2011.##Simmons DG, Cross JC. Determinants of trophoblast lineage and cell subtype specification in the mouse placenta. Dev Biol. 2005; 284(1):12-24.## Cross JC, Nakano H, Natale DR, Simmons DG, Watson ED. Branching morphogenesis during development of placental villi. Differentiation. 2006;74(7):393-401.## Luciani F, Spada M, De Milito A, Molinari A, Rivoltini L, Montinaro A, Marra M, Lugini L, Logozzi M, Lozupone F, Federici C, Iessi E, Parmiani G, Arancia G, Belardelli F, Fais S. Effect of proton pump inhibitor pretreatment on resistance of solid tumors to cytotoxic drugs. J Nat Cancer Inst. 2004; 96 (1): 1702–13.## ##

Investigation of UV/TiO2-N photocatalytic degradation of AR 40 using response surface methodology (RSM) 2 2 Introduction: Advanced oxidation processes (AOPs) suggest a highly reactive, nonspecific oxidant namely hydroxyl radical (OH•), that oxidize a wide range of pollutants fast and non-selective in wastewater and water. Materials and methods: In this work, the nitrogen-doped titanium dioxide nanoparticles were primed by sol-gel method, characterized by X-ray diffraction and Scanning Electron Microscopy (SEM), for the degradation of Acid Red 40 (AR 40) solution in water. The effectiveness of the treatment method applied for the degradation of AR 40 based on AOPs was investigated. Results: The three various key parameters were optimized by using response surface modeling, namely: pH, TiO2-N concentration and the initial AR 40 concentrations. The optimized values were obtained at pH = 11, TiO2-N concentration = 0.09 g/L, and the initial AR 40 concentration = 19 mg/L. Conclusion: Under the optimum conditions, performance of photocatalytic degradation reaches 92.47% in 1 hr. Kinetic constant was evaluated using first-order equation to obtain the rate constant, K. 29 40 2016/02/282016/04/142016/01/312016/03/252016/05/12016/06/1 1395/3/12 2016/08/142016/07/22016/07/152016/08/82016/08/172016/08/4 1395/5/14 Mohsen Nademi Mohsen Nademi Department of Chemical Engineering, Islamic Azad University, Tehran North Branch, Tehran, Iran Iran mohsen.nademi@yahoo.com Mostafa Keshavarz Moraveji Mostafa Keshavarz Moraveji Department of Chemical Engineering, Amirkabir University of Technology (Tehran Polytechnic), Tehran, Iran Iran maraz2010@gmail.com Mohsen Mansouri Mohsen Mansouri Department of Chemical Engineering, Ilam University, Ilam, Iran Iran mansouri2010@yahoo.com Photocatalytic degradation AR 40 TiO2-N nanoparticles Response surface modeling Chong MN, Jin B, Chow CWK, Saint C. Recent developments in photocatalytic water treatment technology: A review. Water Res. 2010; 44(10): 2997-3027. ##Shan AY, Ghazi TIM, Rashid, SA. Immobilisation of titanium dioxide on to supporting materials in heterogeneous photocatalysis: A review. Appl Catal A Gen. 2010; 389(1-2): 1-8. ##Rajeshwar K, Osugi ME, Chanmanee W, Chenthamarakshan CR, Zanoni MWB, Kajitvichyanukul P, Krishnan-Ayer R. Heterogeneous photocatalytic treatment of organic dyes in air and aqueous media. J Photochem Photobiol C Photochem Reviews. 2008; 9(4): 171-92.##Arana J, Peña Alonso A, Doña Rodríguez JM, Herrera Melián JA, González Díaz O, Pérez Peña J. Comparative study of MTBE photocatalytic degradation with TiO2 and Cu-TiO2. Appl Catal B 2008; 7(8): 355-63.##Colon G, Maicu M, Hidalgo MS, Navio JA. Cu-doped TiO2 systems with improved photocatalytic activity. Appl Catal B Environ. 2006; 67(1): 41-51.## Liqianga J, Hongganga F, Baiqia W, Dejunb W, Baifua X, Shudana L, Jiazhong S. Effects of Sn dopant on the photoinduced charge property and photocatalytic activity of TiO2 nanoparticles. Appl Catal B. 2006; 6(2): 282-91.## Chang SM, Doong RA. Characterization of Zr-doped TiO2 nanocrystals prepared by a nonhydrolytic sol-gel method at high temperatures. J Phys Chem B. 2006; 110(42): 20808-14.##Luo H, Takata T, Lee Y, Zhao J, Domen K, Yan Y. Photocatalytic activity enhancing for titanium dioxide by co-doping with bromine and chlorine. Chem Mater. 2004; 16(5): 846-9.## Vinu R, Madras G. Synthesis and photoactivity of Pd substituted nano TiO2. J Mol Catal A Chem. 2008; 291 (1): 5–11.## Sahoo C, Gupta AK, Pal A. Photocatalytic degradation of Methyl Red dye in aqueous solutions under UV irradiation using Ag doped TiO2. Desalination. 2005; 181(1-3): 91–100.## Naskar S, Pillay SA, Chanda M. Photocatalytic degradation of organic dyes in aqueous solution with TiO2 nanoparticles immobilized on foamed polyethylene sheet. J Photochem Photobiol A Chem. 1998; 113(3): 257–64.##Gupta VK, Jain R, Mittal A, Saleh TA, Nayak A, Agarwal S, Sikarwar S. Photo-catalytic degradation of toxic dye amaranth on TiO2/UV in aqueous suspensions. Mater Sci Eng C. 2012; 32(1): 12–7.## Ochuma IJ, Fishwick RP, Wood J, Winterbottom JM. Optimisation of degradation conditions of 1,8-diazabicyclo[5.4.0]undec-7-ene in water and reaction kinetics analysis using a concurrent down flow contactor photocatalytic reactor. Appl Catal B Environ. 2007; 73(3-4): 259-68. ## Bhargava, A., Kabir MF, Vaisman E, Langford CH, Kantzas A. Novel technique to characterize the hydrodynamics and analyze the performance of a fluidized-bed photocatalytic reactor for wastewater treatment. Ind Eng Chem Res. 2004; 43(4): 980-9. ## Toor AP, Verma A, Jotshi CK, Bajpai PK, Singh V. Photocatalytic degradation of Direct Yellow 12 dye using UV/TiO2 in a shallow pond slurry reactor. Dyes and Pigments. 2006; 68(1): 53-60. ## Chin SS, Lim TM, Chiang K, Fane AG. Factors affecting the performance of a low-pressure submerged membrane photocatalytic reactor. Chem Eng J. 2007; 130(1): 53-63. ## Sakkas VA, Islam MA, Stalikas C, Albanis TA. Photocatalytic degradation using design of experiments: A review and example of the Congo red degradation. J Hazard Mater. 2010; 175(1-3): 33-44. ## Pirkaram A, Olya ME, Farshid SR. UV/Ni–TiO2 nanocatalyst for electrochemical. Water Resours Ind. 2014; 5: 9-20.##Zhang J, Fu D, Xu Y, Liu C. Optimization of parameters on photocatalytic degradation of chloramphenicol using TiO2 as photocatalyist by response surface methodology. J of Environ Sci. 2010; 22(8): 1281-9.## Ferreir SLC, Bruns RE, Ferreira HS, Matos GD, David JM, Brandao GC, da Silva EGP, Portugal LA, dos Reis PS, Souza AS, dos Santos WNL. Box-Behnken design: an alternative for the optimization of analytical methods. Anal Chim Acta. 2007; 597(2): 179–186.## Ay F, Catalkaya EC, Kargi F. A statistical experiment design approach for advanced oxidation of Direct Red azo-dye by photo-Fenton treatment. J Hazar Mater. 2009; 162(1): 230-6.##Wang JP, Chen YZ, Wang Y, Yuan SJ. Yu HQ. Optimization of the coagulation-flocculation process for pulp mill wastewater treatment using a combination of uniform design and response surface methodology. Water Research. 2011; 45(17): 5633-40.## Zhou M, Yu J, Cheng B. Effects of Fe-doping on the photocatalytic activity of mesoporous TiO2 powders prepared by an ultrasonic method. J Hazar Mater. 2006; 137(3): 1838-47.## Garcia, J. C., & Takashima, K. ().Photocatalytic degradation of imazaquin in an aqueous suspension of titanium dioxide. Journal of Photochemistry and Photobiology A: Chemistry. 2003; 155(1): 215-22.## Eslami, A., Nasseri S, Yadollahi B, Mesdaghinia A, Vaezi F, Nabizadeh R, Nazmara S. Photocatalytic degradation of methyl tert‐butyl ether (MTBE) in contaminated water by ZnO nanoparticles. J Chem Technol Biotechnol. 2008; 83(11): 1447-53.## Nikazar M, Gholivand K, Mahanpoor K. Photocatalytic degradation of azo dye Acid Red 114 in water with TiO2 supported on clinoptilolite as a catalyst. Desalination. 2008; 219(1-3): 293-300. ##Wang JP, Chen YZ, Wang Y, Yuan S, Yu HQ. Optimization of the coagulation-flocculation process for pulp mill wastewater treatment using a combination of uniform design and response surface methodology. Water Research. 2011; 45(17): 5633–40.## Zhang J, Fu D, Xu Y, Liu C. Optimization of parameters on Photocatalytic degradation of chloramphenicol using TiO2 photocatalyst by response surface methodology. J Environ Sci. 2010; 12(8): 1281–9.## Ferreira SL, Bruns RE, Ferreira HS, Matos GD, David JM, Brandao GC, da Silva EG, Portugal LA, dos Reis PS, Souza AS, dos Santos WN. Box-Behnken design: an alternative for the optimization of analytical methods. Anal Chim Acta. 2007; 597(2): 179–86.## Tonga T, Zhanga J, Tiana B, Chena F, Heb D. Preparation of Fe3+-doped TiO2 catalysts by controlled hydrolysis of titanium alkoxide and study on their Photocatalyticactivity for methyl orange degradation. J Hazard Mater. 2008; 155(3): 572-9.## Hu Q, Zhang C, Wang Z, Chen Y, Mao K, Zhang X, Xiong Y, Zhu M. Photodegradation of methyl tert-butyl ether (MTBE) by UV/H2O2 and UV/TiO2. J Hazard Mater 2008; 154(1-3): 795–803.##Safari M, Nikazar M, Dadvar M. Photocatalytic degradation of methyl tert-butyl ether (MTBE) by Fe-TiO2 nanoparticles. J Ind Eng Chem. 2013; 19(5): 1697–702.## ##

The impact of fatigue on balancing in creating re-injuries in mountaineers with chronic ankle sprain 2 2 Introduction: Sensory-motor control and in general gaining balance will be impaired after an ankle injury and in fatigue situations. The aim of this research was to study the effect of exercise-based fatigue on static and dynamic balance in mountaineering athletes who were successful in returning to a suitable level of physical activity after an ankle sprain injury. Materials and methods: 25 active mountaineering athletes between 20 and 40 years were recruited on a voluntary basis. 12 persons with severe ankle sprain in the past were selected as experimental group and 13 healthy mountaineering athletes were selected as control group. Because balance test measuring was under the influence of evaluation time; fatigue test measurement running on the treadmill was performed in 2 sessions. Changes or fluctuations in the pressure Center or static balance were evaluated by Sharpened Romberg balance test and dynamic balance was evaluated by stability time after side jumping (the first session) and the maximum distance in the balance test Y (second session) were evaluated in the pre-test and post-test immediately after fatigue resulted by running on a treadmill. Data were analyzed using covariance test. Results: before fatigue condition, there were a few differences between the two groups in stability time while doing side jump test (P<0.014). After the fatigue and in balance test Y and stability time while doing side jump test; there were more differences (P<0.001). In this case, there was a similar increase on static swing in both groups. Fatigue was considerably reduced the static and dynamic postural control of the athletes who had a history of injury. Changes related to fatigue was significant in dynamic postural control in athletes with previous ankle sprain history. Conclusion: Even after the successful return to sport for athletes with previous damages, abnormalities in sensory-motor control and balance may lead to an increase the risk of ankle re-injury. 41 49 2016/02/282016/04/142016/01/312016/03/252016/05/12016/06/12015/09/10 1394/6/19 2016/08/142016/07/22016/07/152016/08/82016/08/172016/08/42015/12/16 1394/9/25 Himan Ebrahimi Himan Ebrahimi Department of Sport Pathology and Corrective Exercise, Faculty of Physical Education and Sport Sciences, University of Allameh Tabatabai, Tehran, Iran Iran ebrahimihiman@yahoo.com Mehdi Shahrokhi Mehdi Shahrokhi Department of Sport Pathology and Corrective Exercise, Faculty of Physical Education and Sport Sciences, University of Allameh Tabatabai, Tehran, Iran Iran mehdi_mk67@yahoo.com Zahra Toktam Barmar Zahra Toktam Barmar Department of Physical Education and Sports Science, Islamic Azad University of Mashhad, Mashhad, Iran Iran barmar.zahra67@gmail.com Ramin Balouchi Ramin Balouchi Department of Physical Education and Sports Science, Islamic Azad University of Mashhad, Mashhad, Iran Iran ram_b81@yahoo.com Ankle sprain Balance Fatigue Mountain climbers Garric JG. The frequency of injury, mechanism of injury, and epidemiology of ankle sprains. Am J Sports Med. 1977; 5(6):241-2.## Kofotolis N, Kellis E, Symeon P. Ankle sprain Injuries and Risk Factors in Amateur Soccer Players During a 2-Year Period. Am J Sports Med. 2007; 35(3): 458-66.##Holmer P, Sondergaard L, Konradsen L, Nielsen PT, Jorgensen LN. Epidemiology of sprains in the lateral ankle and foot. Foot Ankle Int. 1994; 15(2): 72-4.## Freeman MA. Instability of the foot after injuries to the lateral ligament of the ankle. J Bone Joint Surg Br. 1965; 47(4): 669-77.##Kofotolis N, Kellis E. Ankle sprain injuries: a 2–years prospective cohort study in female Greek professional basketball players. J Athl Train. 2007; 42(3):388-94.## Alexander C. Exercise Physiology.3rd edition, Printed in United States of America. 1998; 226-9.## McCriskin B, Cameron K, Orr J, Waterman B. Management and prevention of acute and chronic lateral ankle instability in athletic patient populations. World J Orthop. 2015; (6)2: 161-71## Lourok S, Weiss E. Burnnstorms clinical kinesiology. 4th ed. F.A Davis company.1983; 111-3.## Beynnon B, Murphy F, Alosa D. Predictive factors for lateral ankle sprains: a literature review. J Athl Train. 2002; 37(4):376-80.## Nelson A, Collins C, Yard E, Fields S, Comstock R. Ankle injuries among United States high school sports athletes. J Athl Train. 2007; 42(3):381-7.## Fuller C, Ekstrand J, Junge A, Anderse T, Bahr R, Dvorak J. Consensus statement on injury definitions and data collection procedures in studies of football (soccer) injuries. Br J Sports Med. 2006; 40(3):193-201.## McKay G, Goldie P, Payne W, Oakes B. Ankle injuries in basketball: injury rate and risk factors. Br J Sports Med. 2001; 35(2):103-8.## Jackson D, Ashley R, Powell J. Ankle Sprains in Young Athletes: Relation of Severity and Disability. Clin Orthop Relat Res. 1974; 101(2):201-15.## Docherty C, Arnold B, Gansneder B, Hurwitz S, Gieck J. Functional-performance deficits in volunteers with functional ankle instability. J Athl Train. 2005; 40(1):30-4## Lauren C, Christopher R, Hertel J, Sandra J. Efficacy of the star excursion balance tests in detecting reach deficits in subjects with chronic ankle instability. J Athl Train. 2002; 37(4): 501-6.##Sekir U, Yildiz Y, Hazneci B, Ors F, Aydin T. Effect of isokinetic training on strength, functionality and proprioception in athletes with functional ankle instability. Knee Surg Sports Traumatol Arthrosc. 2007; 15(5):654-64.## Munn J, Beard D, Refshauge K, Lee R. Do functional-performance tests detect impairment in subjects with ankle instability? J Sport Rehabil. 2002; 11(1): 40-50.## Kerry M, Sandra J, Docherty C, Gansneder B, Perrin D. Chronic Ankle Instability Does Not Affect Lower Extremity Functional Performance. J Athl Train. 2002; 37(4): 507–11.##Engebretsen A, Myklebust G, Holme I, Engebretsen L, Bahr R. Intrinsic risk factors for acute ankle injuries among male soccer players: a prospective cohort study. Scand J Med Sci Sports. 2010; 20(3):403-10.## Yim-Chiplis P, Talbot L. Defining and measuring balance in adults. Biol Res Nurs. 2000; 1(4): 321-31.## Reiman M, Manske R. Functional testing in human performance. USA: Human Kinetics; 2009; 155-59.## Ortiz A, Olson SL, Roddey T, Morales J. Reliability of selected physical performance tests in young adult women. J Strength Cond Res. 2005; 19(1): 39-44.## Gribble PA, Hertel J, Plisky P. Using the Star Excursion Balance Test to Assess Dynamic Postural-Control Deficits and Outcomes in Lower Extremity Injury: A Literature and Systematic Review. J Athl Train. 2012; 47(3): 339-57.## Plisky PJ, Gorman PP, Butler RJ, Kiesel K, Underwood FB, Elkins B. The reliability of an instrumented device for measuring components of the star excursion balance test. N Am J Sports Phys Ther. 2009; 4(2): 92-9.##Borg G. Perceived exertion as an indicator of somatic stress. Scand J Rehabil Med. 1970; 2(2):92–8.## Gribble PA, Hertel J, Denegar CR, Buckley WE. The effects of fatigue and chronic ankle instability on dynamic postural control. J Athl Train. 2004; 39(4):321–9.##Gandevia SC. Spinal and supraspinal factors in human muscle fatigue. Physiol Rev. 2001; 81(4):1725–89.## Forestier N, Teasdale N, Nougier V. Alteration of the position senseat the ankle induced by muscular fatigue in humans. Med Sci Sports Exerc. 2002; 34(1):117–22.## McLean SG, Fellin RE, Suedekum N, Calabrese G, Passerallo A, Joy S. Impact of fatigue on gender-based high-risk landing strategies. Med Sci Sports Exerc. 2007; 39(3):502–14.## Skinner HB, Wyatt MP, Stones ML, Hodgdon JA, Barrack RL. Exercise-related knee joint laxity. Am J Sports Med. 1986; 14(1):30–4.##Gribble PA, Tucker WS, White PA. Time-of-day influences on static and dynamic postural control. 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Mesenchymal stem cells as a reference cell for HLA-typing 2 2 Introduction: Recognition of human leukocyte antigens (HLA) is of importance for hematopoietic stem cell transplantation. Any HLA-mismatches between the donor and recipient can cause graft rejection or other complications. In HLA-typing experiments, usage of HLA-known reference cells accompany with HLA-unknown samples is obligatory. Some international centers represent these cells with high expenses. On the other hand, transferring of these cells is problematic and in some instances is not practical. In this study, we introduced umbilical cord-derived mesenchymal stem cells (MSCs) as reference cells for HLA genotyping. These cells are national and can be prepared locally. Materials and methods: We isolated MSCs from three umbilical cord and after their growth and proliferation, these cells were characterized by flow cytometry technique using antibodies to CD29, CD34, CD44, CD45, CD73, CD90 and CD105. HLA-typing was then carried out by PCR-SSP kits for HLA-A, -B and -DRB allele’s identification. Results: Isolated MSCs were positive for MSCs markers; CD29, CD44, CD73, CD90, and CD105 and negative for hematopoietic stem cell markers; CD34 and CD45. HLA alleles were determined. One of the samples was homologous for HLA alleles and the others were heterologous. Conclusion: We can develop a reference panel for HLA-typing by obtaining MSCs from available sources like umbilical cord. 50 57 2016/02/282016/04/142016/01/312016/03/252016/05/12016/06/12015/09/102016/01/2 1394/10/12 2016/08/142016/07/22016/07/152016/08/82016/08/172016/08/42015/12/162016/07/1 1395/4/11 Roohollah Mirzaee Khalilabadi Roohollah Mirzaee Khalilabadi Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran Iran khalilabadi60@gmail.com Fatemeh Yari Fatemeh Yari Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran Iran f.yari@ibto.ir Naser Amirizadeh Naser Amirizadeh Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran Iran n.amirizadeh@ibto.ir Mahin Nikougoftar Mahin Nikougoftar Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran Iran nikougoftar@gmail.com Arezoo Oodi Arezoo Oodi Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran Iran a.oodi@ibto.ir HLA-typing Mesenchymal stem cells Reference panel Umbilical cord Mahdi BM. A glow of HLA typing in organ transplantation. Clin Transl Med. 2013; 2(1):6.## Fernandez-Viña MA. HLA factors in transplantation for nonmalignant hematologic disorders. Blood. 2012; 120(14): 2781-2. ## Hurley CK, Wade JA, Oudshoorn M, Middleton D, Kukuruga D, Navarrete C, et al. A special report: histocompatibility testing guidelines for hematopoietic stem cell transplantation using volunteer donors. Tissue Antigens. 1999; 53: 394-406.## Erlich HA, Opelz G, Hansen J. HLA DNA typing and transplantation. Immunity. 2001; 14(4): 347-56.##5. Koo HH, Ahn HS. Umbilical cord blood transplantation. Korean J Pediatr. 2012; 55(7): 219-23.## Horan J, Wang T, Haagenson M, Spellman SR, Dehn J, Eapen M, et al. Evaluation of HLA matching in unrelated hematopoietic stem cell transplantation for nonmalignant disorders. Blood. 2012; 120(14): 2918-24. ## Middelton D. HLA Typing from Serology to Sequencing Era. 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