2024-03-28T23:27:10+04:30 http://jbrms.medilam.ac.ir/browse.php?mag_id=22&slc_lang=en&sid=1
22-385 2024-03-28 10.1002
Journal of Basic Research in Medical Sciences 2383-0506 2383-0972 doi 2019 6 2 Effect of injection of diazepam, melatonin, methocarbamol and glucose on physiological responses of neonate chicks subjected to road transportation stress Kianoosh Zarrinkavyani k.zarrinkavyani@gmail.com Heshmatolah Khosravinia khasravi2241@gmail.com Gholamreza Shahsavari Zahra Biranvand Introduction: Transportation causes stress in chicks that may make numerous physiological changes with a negative impact on their production and health. This study was conducted to examine the effects of injection of diazepam, melatonin, methocarbamol and glucose on Serum biochemical parameters include: glucose, total protein, albumin, uric acid, cholesterol, triglycerides, Ca, P, Zn, Fe, Na and K in neonate chicks subjected to road transportation stress. Materials and methods: The research was carried out in the form of factorial experiment (9×5) based on a completely randomized design with 4 replications. A total of 940 one-day old Ross 308 Male-sex broiler chicks were bought from a commercial hatchery. Immediately after the hatching, broiler chicks were traveled to about 1200 km far from the hatchery. At hatchery (40 samples) and after 300, 600, 900, and 1200 km journey, a random sample (From each treatment 8 birds) were sampled for blood collection. Results: The mean concentration of glucose, protein, albumin, cholesterol, triglyceride, calcium, phosphorus, iron, sodium, and potassium was not affected by the treatments (P>0.05), but concentration of uric acid and plasma Zn was influenced (P<0.05). The effect of road distance on all serum parameters except Zn was significant. Furthermore, the mean concentrations of uric acid, Zn, Sodium and Potassium in serum showed an interaction between treatments and transport distance (P<0.05). Conclusion: These results clearly show the physiological responses of chickens under long-distance transport and the effect of some treatments on stress indicators. Neonate chicks Transportation stress Physiological parameters 2019 3 01 1 10 http://jbrms.medilam.ac.ir/article-1-385-en.pdf
22-377 2024-03-28 10.1002
Journal of Basic Research in Medical Sciences 2383-0506 2383-0972 doi 2019 6 2 The effect of aerobic training and consumption of L-carnitine supplements on HMG-CoA reductase and LDL receptor in the liver of male wistar rats toxicated by boldenone Behnam Bagherzadeh Rahmani b.bagherzadeh@gmail.com Asieh Abbassi Daloii abbasi.daloii@gmail.com Introduction: The aim of this study was to investigate the effect of aerobic training and consumption of L-carnitine supplements on HMG-CoA reductase and low density lipoprotein receptor (LDL-R) in the liver of male Wistar rats toxicated by boldenone. Materials and methods: In this clinical study, 30 male Wistar rats aged 12 weeks (weight 195±7.94g) were randomly divided into five groups: control, no-treatment, boldenone (5mg per kg), L-carnitine and L-carnitine + training groups with six rat in each group. The moderate intensity endurance training program (50-55% of maximal oxygen consumption) performed for 6 weeks and 5 times a week. Injection once a week, on an appointed day, and in the quadriceps and hamstring was conducted in depth. After anesthesia, biopsy in aliquots was prepared. The HMG-CoA reductase and LDL-R expression in the samples was measured by Real-Time-PCR and the quantification of gene expression levels was calculated using the formula 2-ΔΔct then analyzed by One-way ANOVA and post hoc Scheffe at P<0.05. Results: The results showed that aerobic training and supplementation with L-carnitine had significant effects on HMG-CoA reductase and LDL-R in the liver of male Wistar rats intoxicated by boldenone (P=0.0001). The results showed that the expression of HMG-CoA reductase in training-L-carnitine group was significantly lower than the control group (P=0.0001). The expression of LDL-R in training-L-carnitine and L-carnitine group increased significantly compared to control group (P=0.0001). Conclusion: According to the findings, it seems to the supplementation with L-carnitine alongwith regular aerobic training modulate the biosynthesis of cholesterol in liver tissue. Aerobic training Boldenone L-carnitine HMG-CoA reductase LDL receptor Wistar rats 2019 3 01 11 22 http://jbrms.medilam.ac.ir/article-1-377-en.pdf
22-323 2024-03-28 10.1002
Journal of Basic Research in Medical Sciences 2383-0506 2383-0972 doi 2019 6 2 Sox2 gene expression as a gene to change the aggressive behavior of cancerous cells in patients with gastric cancer Fatemeh Keshavarzi gol.keshavarzi@gmail.com Shahnaz Chaghakaboodi Abdolrasool Khalafi Introduction: Epithelial–mesenchymal transition (EMT) phenomenon in cancer cells is one of the most sensitive stages in metastasis. In addition, it has been shown that many molecular factors and cellular signals, including the Sox2 gene, are involved in arising EMT, which together cause EMT. The phenomenon of EMT in cancer cells is one of the most sensitive stages in metastasis. Expression of Sox2 gene as an effective gene in altering aggressive behavior of cancer cells has not been studied in patients with gastric cancer. Materials and methods: RNA was extracted from 50 tumoral and 50 normal samples from patients with gastric cancer. Then cDNA synthesis was performed on the extracted RNAs. Primers for the target genes were designed and synthesized. Quantitative real-time PCR was performed to determine the relative expression of the studied genes and the relationship between the clinic pathologic parameters and the amount of RNA was analyzed by Pearson correlation coefficient analysis. Results: mRNA expression of Sox2 in tumor tissue was significantly higher than that in healthy adjacent tumor tissue. The mRNA expression level of Sox2 in tumor tissue was significantly higher than that in healthy adjacent tumor tissue.  Also, mRNA levels of Sox2 in tumor tissue indicated a direct and significant correlation with tumor staging (TNM stages) (τ = 0.329, P = 0.02). In addition, there was no significant correlation between mRNA levels of Sox2 in tumor tissue and tumor size (τ = 0.138, P = 0.177). Conclusion: Assessment of Sox2 gene expression in the study of genes involved in EMT process in gastric cancer patients and its relation to pathologic, clinical and metastatic findings in gastric cancer is an effective method in the diagnosis of gastric cancer.   Gastric cancer Sox2 Gene expression 2019 3 01 23 29 http://jbrms.medilam.ac.ir/article-1-323-en.pdf
22-338 2024-03-28 10.1002
Journal of Basic Research in Medical Sciences 2383-0506 2383-0972 doi 2019 6 2 Evaluating the expression of ERG gene in acute myeloblastic leukemia in north east of Iran Hossein Ayatollahi aytollahihossein@yahoo.com Hossein Rahimi rahimih@mums.ac.ir MohammadHadi Sadeghian sadeghianm100@yahoo.com Mohammad Reza Keramati keramatimr@mums.ac.ir Mojgan Amirpour amirpoormojhgan@yahoo.com Maryam Sheikhi sheikhim75@gmail.com Nafiseh Amini aminin3@mums.ac.ir Sepideh Shakeri se.shakeri@yahoo.com Seyyede Fateme Shams shams8869@yahoo.com Zahra Zafari Somaieh Azarkerdar somaiehazarkerdar@yahoo.com Introduction: Erythroblast transformation specific Related Gene (ERG) is a member of ETS transcription factors family, which act as a regulator of primary hematopoietic cell differentiation. Previous researches suggested proto-oncogene for response to mitogenic signal of different cell with MAP kinase and help to tumorgenesis, apoptotic and prognostic role for ERG gene in acute myeloid leukemia (AML) patients. The aim of his study was to evaluate ERG gene expression level in AML cases compare to control group. Materials and methods: For analysis of gene expression, total RNA was isolated from bone marrow (BM) and peripheral blood of AML `s patient. This study was conducted on 54 AML patients and 54 healthy individuals as control group. ERG gene expression and reference gene glucose-6-phosphate isomerase (GPI) was assessed by using real-time polymerase chain reaction (RT-PCR) method. Clinical and laboratory findings studied cases were extracted from medical documents. Results: This study results indicated that the level of ERG gene expression in AML patients had no significant differences compare to control group (1.81±2.41) (P <0.05), Also ERG gene expression was correlated to patients hematocrit (P <0.05). Conclusion: This research recommends that there were no significant relation between ERG expression and control groups. It seems that ERG gene assessment is not a good index for AML patient’s evaluation. ERG gene Acute myeloid leukemia RT-PCR method 2019 3 01 30 35 http://jbrms.medilam.ac.ir/article-1-338-en.pdf
22-420 2024-03-28 10.1002
Journal of Basic Research in Medical Sciences 2383-0506 2383-0972 doi 2019 6 2 Verbal inflection deficit in Kurdish-speaking aphasia patients Kazhal Feilinezhad Habib Gowhari Kurosh Sayemiri Tayebeh Khoshbakht Introduction: Aphasia is a language disorder which can affect different aspects of language production and comprehension. Broca’s aphasia is a non-fluent type of aphasia in which patients suffer from a deficit in the use of function words and inflectional categories of the verb. Materials and methods: Two Kurdish- Speaking patients diagnosed with Broca’s Aphasia were selected to be investigated for their linguistic performance in the main inflectional categories of verbs including grammatical agreement, tense and aspect. To collect the data, natural conversations of the patients were recorded to be analyzed. Sentence Completion Task (SCT) was, also, employed to find the less frequent inflectional categories. SPSS version 23 was utilized to analyze the collected corpora. Results: Both patients had a relatively intact agreement system, while both committed a higher number of errors as far as grammatical tense is concerned. Finally, the worst linguistic performance for the two patients was reported to be in the appropriate use of markers to represent grammatical aspect. Conclusion: Grammatical aspect was found to be the most problematic inflectional category, while grammatical agreement was the least severely damage category for Kurdish-speaking aphasic patients. Broca’ aphasia Inflectional category Agreement Tense Aspect 2019 3 01 36 43 http://jbrms.medilam.ac.ir/article-1-420-en.pdf
22-423 2024-03-28 10.1002
Journal of Basic Research in Medical Sciences 2383-0506 2383-0972 doi 2019 6 2 Correlation of mental disorders with religious attitude and mental health among the elderly of Ilam city Kourosh Sayehmiri Sayehmiri@razi.tums.ac.ir Mandana Sarokhani sarokhani_m@yahoo.com Milad Bagheri Reza Ghanei Gheshlagh Ghanei@muk.ac.ir Introduction: The elderly are susceptible to mental disorders due to poor physical and mental ability, loss of relatives and poor social communication. Mental health and religious attitudes are factors that may be associated with mental disorders in the elderly. This research was aimed to explore the relationship of mental disorders with mental health and religious attitudes among the elderly. Materials and methods: This descriptive, correlational study was conducted on 400 elderly in Ilam, Iran, who were selected by convenience sampling. Data were collected by the Symptom Checklist-90-R (SCL-90-R), General Health Questionnaire (GHQ-28) and Religious Attitude Questionnaire (Gloris-Brahni). The obtained data were analyzed by PASW software using descriptive statistics, Pearson correlation coefficient and stepwise regression analysis. P<0.05 was considered significant. Results: The results showed a significant correlation between mental disorders and mental health (P=0.003, r=0.147) and religious attitude (P=0.001, r=-0.187). The results of regression analysis to predict mental disorders based on the predictor variables indicated that religious attitude could predict 4.6% of mental disorders in the Kurdish elderly. In the next steps, the marital status, gender and mental health variables were included in the model, which could generally predict 8.2% of changes in mental disorders among the elderly of Ilam city. Conclusion: Religious attitude and mental health could predict mental disorders in the elderly. It seems necessary to gain a better understanding of the risk factors of mental disorders in the elderly to design interventional programs and to identify the people at risk. Elderly Attitude Religious Mental disorders Mental health 2019 3 01 44 50 http://jbrms.medilam.ac.ir/article-1-423-en.pdf
22-421 2024-03-28 10.1002
Journal of Basic Research in Medical Sciences 2383-0506 2383-0972 doi 2019 6 2 Frequency of Neisseria gonorrhoeae and Trichomonas vaginalis in women with vaginal discharge referring to the gynecology clinic of Vali-e-Asr Hospital in Birjand in 2018 Nahid Ghanbarzade Marzie Moghanni Negar Goljahani Mehdi Karamian Majid Zare_Bidaki ntroduction: Neisseria gonorrhoeae (N. gonorrhoeae) and Trichomonas vaginalis (T. vaginalis) are the most common sexually transmitted microorganisms. Contamination with these microorganisms is a global problem, such that T. vaginalis causes complications in pregnancy and increases the risk of HIV transmission. N. gonorrhoeae causes pelvic infections and can result in complications such as infertility, chronic pelvic pain, ectopic pregnancy, and preterm delivery. In this study, the frequency of these two microorganisms and their related factors was investigated. Materials and methods: This descriptive-analytic study was performed in the gynecology clinic affiliated with Vali-e-Asr Hospital of Birjand during a one-year period (2018). A total of 400 women with abnormal vaginal discharge were included. Sterile swabs were used to take samples from the vaginal area, and the specimens were mounted on four microscope slides. One of the slides was used to detect vaginal Trichomoniasis by wet mount, and three other slides were stained via Giemsa (for diagnosis of vaginal discharge), Gram, and methylene blue staining methods (for detection of N. gonorrhoeae). Data analysis was performed in SPSS software, using Chi-square and Fisher's exact tests. Results: The mean age of the patients was 32.67 ± 8.24 years. The percentage of infliction with N. gonorrhoeae and T. vaginalis were 1.75% and 6.75%, respectively. Statistical analysis did not show a significant difference between the prevalence of T. vaginalis infection and different factors such as age, education level, place of residence, marital status, and contraceptive method (P < 0.05). Conclusion: The relative frequency of infection with N. gonorrhoeae in Birjand appears to be within the predicted global range, while the frequency of T. vaginalis is more than the expected range. It is recommended that healthcare professionals be trained for timely prevention and treatment of people with signs, notification, education, and screening. Neisseria gonorrhoeae Trichomonas vaginalis Microscopic observation Genitourinary infection 2019 3 01 51 57 http://jbrms.medilam.ac.ir/article-1-421-en.pdf
22-405 2024-03-28 10.1002
Journal of Basic Research in Medical Sciences 2383-0506 2383-0972 doi 2019 6 2 Antibiotic resistance of Citrobacter freundii in clinical isolates: A systematic review and meta-analysis Arman Rostamzad arostamzad381@yahoo.com Aboozar Karami Cherag Abad akerami.95@gmail.com Mehdi Omidi omidi_280@yahoo.com Aliasghar Hatamnia hatamniya60@gmail.com Introduction: Citrobacter freundii (C. freundii) is an opportunistic infection agent in hospitalized patients, especially in the intensive care units (ICUs). The prevalence of antibiotic resistance in C. freundii is increasing in the worldwide, and this may cause significant clinical problems. This paper aims at evaluating the rate of antibiotic resistance of C. freundii using meta-analysis. Materials and methods: A total of eight qualified antibiotics in 21 published articles were chosen randomly to estimate the antibiotic resistance percentage of C. freundii in different countries. The data were analyzed by meta-analysis (random effect model) and the heterogeneity was determined using Cochran’s Q and I2 index. Also, Forest plot with confidence interval 95% were attained by R software. Results: Our data showed that the antibiotic resistance pattern of C. freundii in different parts of the world was not absolutely same but the maximum range of resistance was related to gentamicin and the minimum range of resistance was related to imipenem. Q and I2 were attained 45.25 (P<0.05) and 56%, respectively. Conclusion: The findings of this study showed that the variability in antibiotic resistance pattern of C. freundii in different studies across the world was due to heterogeneity in hygiene level that resulted from various geographic regions. Citrobacter freundii Antibiotic resistance Nosocomial pathogen 2019 3 01 58 63 http://jbrms.medilam.ac.ir/article-1-405-en.pdf