Journal of Basic Research in Medical Sciences
J Basic Res Med Sci
Volume 13, Issue 4 (9-2026)
jbrms 2026, 13(4): 49-57 |
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Mohammadi R, Vatannejad A, Kheirollahi A. Impact of Folate Supplementation on Liver Oxidative Stress Indicators in Diabetic Rats Induced by Streptozotocin. jbrms 2026; 13 (4) :49-57
URL: http://jbrms.medilam.ac.ir/article-1-1077-en.html
URL: http://jbrms.medilam.ac.ir/article-1-1077-en.html
Department of Comparative Biosciences, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran , vatannejad@ut.ac.ir
Abstract: (20 Views)
Introduction: Diabetes can cause metabolic disorders and the liver is an organ at risk due to its crucial role in glucose and lipid metabolism. Oxidative stress and inflammation play key roles in the development of diabetic liver disease. Folate, an antioxidant water-soluble B-vitamin, might prove beneficial in the treatment of these conditions through decreasing oxidative stress and regulating the metabolism of homocysteine. The present study was designed to investigate the effect of folate supplementation on glucose metabolism, redox status and liver dysfunction in streptozotocin-induced diabetic rats.
Materials & Methods: Adult male Wistar rats (200 +/- 20 g) were randomly divided into three groups (n=6): control, STZ-induced diabetic and folate-treated diabetic. Diabetic status in the two diabetic groups was induced by a single intraperitoneal (i.p.) injection of streptozotocin (55 mg/kg). Folate (15 mg/kg, i.p.) was administered once daily for 4 weeks in the therapy group. At end of the study, fasting blood sugar (FBS), serum liver enzymes, hepatic MDA, SOD activity and TAC were determined. Data were analyzed by one-way ANOVA and Dunnett’s post hoc test.
Results: Folate supplementation resulted in mild improvement in body weight, FBS, ALT and AST levels in diabetic rats. However, these changes were not significant when compared to untreated diabetic animals. There were no significant changes in the hepatic levels of MDA and TAC, and a mild non-significant increase in SOD activity after the administration of folate.
Conclusion: Folate exerted small, possibly beneficial effects on metabolic and hepatic oxidative stress in STZ-induced diabetic rats. The slight improvement in antioxidant status, although not statistically significant, suggests that folate may have protective effects in diabetic conditions. Its therapeutic efficacy may be enhanced when used as an adjunct to conventional antidiabetic treatments.
Materials & Methods: Adult male Wistar rats (200 +/- 20 g) were randomly divided into three groups (n=6): control, STZ-induced diabetic and folate-treated diabetic. Diabetic status in the two diabetic groups was induced by a single intraperitoneal (i.p.) injection of streptozotocin (55 mg/kg). Folate (15 mg/kg, i.p.) was administered once daily for 4 weeks in the therapy group. At end of the study, fasting blood sugar (FBS), serum liver enzymes, hepatic MDA, SOD activity and TAC were determined. Data were analyzed by one-way ANOVA and Dunnett’s post hoc test.
Results: Folate supplementation resulted in mild improvement in body weight, FBS, ALT and AST levels in diabetic rats. However, these changes were not significant when compared to untreated diabetic animals. There were no significant changes in the hepatic levels of MDA and TAC, and a mild non-significant increase in SOD activity after the administration of folate.
Conclusion: Folate exerted small, possibly beneficial effects on metabolic and hepatic oxidative stress in STZ-induced diabetic rats. The slight improvement in antioxidant status, although not statistically significant, suggests that folate may have protective effects in diabetic conditions. Its therapeutic efficacy may be enhanced when used as an adjunct to conventional antidiabetic treatments.
Keywords: Diabetes, Oxidative Stress, Liver Function, Folate, Malondialdehyde, Total Antioxidant Capacity, Streptozotocin
Type of Study: Research |
Subject:
Clinical Biochemistry
Received: 2026/05/9 | Accepted: 2026/06/23 | Published: 2026/09/28
Received: 2026/05/9 | Accepted: 2026/06/23 | Published: 2026/09/28
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