Journal of Basic Research in Medical Sciences
J Basic Res Med Sci
Volume 13, Issue 4 (9-2026)
jbrms 2026, 13(4): 29-38 |
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Nobakht M, Hashemi P, Kazemzadeh S, Safarkhani K, Babakhani A. Effects of Hair Follicle Stem Cells and Simvastatin on Deep Partial Thickness Wound Healing: Histological and Molecular Assay. jbrms 2026; 13 (4) :29-38
URL: http://jbrms.medilam.ac.ir/article-1-1078-en.html
URL: http://jbrms.medilam.ac.ir/article-1-1078-en.html
Department of Anatomy, Faculty of Medicine, Ilam University of Medical Sciences, Ilam Iran , azarbabakhani58@gmail.com
Abstract: (20 Views)
Introduction: Restoration of burn wounds in a shorter time with fewer side effects is one of the main concerns of wound management. In this study, the effects of simultaneous use of hair follicle stem cells (HFSCs) and simvastatin (SMV) in partial-thickness burn wound healing and C-X-C motif chemokine ligand 12 (CXCL12) / C-X-C chemokine receptor type 4 (CXCR4) and vascular endothelial growth factor (VEGF) expression are investigated.
Materials & Methods: Animals (male Wistar rats) were categorized into five groups: 1) control (non-treated), 2) vehicle, 3) cell (HFSCs), 4) drug (SMV), and 5) cell-drug (HFSCs + SMV). The bulge of rat whisker was isolated and cultured in DMEM/F12 and transplanted to the burn-wound site. Daily dressing was performed with petrolatum gel containing 5 μM simvastatin. At the end of therapeutic intervention, histological assessment (H&E), molecular assay (p-value blot), and tensile strength assay were performed. Statistical analyses were performed using GraphPad Prism version 6.01 (two-way repeated-measures ANOVA, one-way ANOVA, and Tukey’s post-hoc test). A p-value was considered statistically significant. Quantitative analysis of wound healing parameters was performed using ImageJ 1.46r software.
Results: The diameter of epidermis, dermis structural integrity, and expression of SDF-1/CXCR4 and VEGF in cell-drug groups were significantly improved compared with other groups (P < 0.05). No significant differences were observed between the vehicle and control groups.
Conclusion: Hair follicle stem cell transplantation simultaneously with simvastatin treatment accelerates the healing of the burn wounds and skin tensile strength by elevating the SDF-1 and VEGF expression levels in the injected region
Materials & Methods: Animals (male Wistar rats) were categorized into five groups: 1) control (non-treated), 2) vehicle, 3) cell (HFSCs), 4) drug (SMV), and 5) cell-drug (HFSCs + SMV). The bulge of rat whisker was isolated and cultured in DMEM/F12 and transplanted to the burn-wound site. Daily dressing was performed with petrolatum gel containing 5 μM simvastatin. At the end of therapeutic intervention, histological assessment (H&E), molecular assay (p-value blot), and tensile strength assay were performed. Statistical analyses were performed using GraphPad Prism version 6.01 (two-way repeated-measures ANOVA, one-way ANOVA, and Tukey’s post-hoc test). A p-value was considered statistically significant. Quantitative analysis of wound healing parameters was performed using ImageJ 1.46r software.
Results: The diameter of epidermis, dermis structural integrity, and expression of SDF-1/CXCR4 and VEGF in cell-drug groups were significantly improved compared with other groups (P < 0.05). No significant differences were observed between the vehicle and control groups.
Conclusion: Hair follicle stem cell transplantation simultaneously with simvastatin treatment accelerates the healing of the burn wounds and skin tensile strength by elevating the SDF-1 and VEGF expression levels in the injected region
Type of Study: Research |
Subject:
Cellular and molecular biology
Received: 2026/05/17 | Accepted: 2026/08/9 | Published: 2026/09/28
Received: 2026/05/17 | Accepted: 2026/08/9 | Published: 2026/09/28
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