[Home ] [Archive]    
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 6, Issue 2 (3-2019) ::
JBRMS 2019, 6(2): 23-29 Back to browse issues page
Sox2 gene expression as a gene to change the aggressive behavior of cancerous cells in patients with gastric cancer
Fatemeh Keshavarzi , Shahnaz Chaghakaboodi , Abdolrasool Khalafi
Department of Biology, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran , gol.keshavarzi@gmail.com
Abstract:   (80 Views)
Introduction: Epithelial–mesenchymal transition (EMT) phenomenon in cancer cells is one of the most sensitive stages in metastasis. In addition, it has been shown that many molecular factors and cellular signals, including the Sox2 gene, are involved in arising EMT, which together cause EMT. The phenomenon of EMT in cancer cells is one of the most sensitive stages in metastasis. Expression of Sox2 gene as an effective gene in altering aggressive behavior of cancer cells has not been studied in patients with gastric cancer.
Materials and methods: RNA was extracted from 50 tumoral and 50 normal samples from patients with gastric cancer. Then cDNA synthesis was performed on the extracted RNAs. Primers for the target genes were designed and synthesized. Quantitative real-time PCR was performed to determine the relative expression of the studied genes and the relationship between the clinic pathologic parameters and the amount of RNA was analyzed by Pearson correlation coefficient analysis.
Results: mRNA expression of Sox2 in tumor tissue was significantly higher than that in healthy adjacent tumor tissue. The mRNA expression level of Sox2 in tumor tissue was significantly higher than that in healthy adjacent tumor tissue.  Also, mRNA levels of Sox2 in tumor tissue indicated a direct and significant correlation with tumor staging (TNM stages) (τ = 0.329, P = 0.02). In addition, there was no significant correlation between mRNA levels of Sox2 in tumor tissue and tumor size (τ = 0.138, P = 0.177).
Conclusion: Assessment of Sox2 gene expression in the study of genes involved in EMT process in gastric cancer patients and its relation to pathologic, clinical and metastatic findings in gastric cancer is an effective method in the diagnosis of gastric cancer.
 
Keywords: Gastric cancer, Sox2, Gene expression
Full-Text [PDF 892 kb]   (17 Downloads)    
Type of Study: Research | Subject: Genetics
Received: 2019/01/2 | Accepted: 2019/04/20
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Keshavarzi F, Chaghakaboodi S, Khalafi A. Sox2 gene expression as a gene to change the aggressive behavior of cancerous cells in patients with gastric cancer. JBRMS. 2019; 6 (2) :23-29
URL: http://jbrms.medilam.ac.ir/article-1-323-en.html


Volume 6, Issue 2 (3-2019) Back to browse issues page
مجله ی تحقیقات پایه در علوم پزشکی Journal of Basic Research in Medical Sciences
Persian site map - English site map - Created in 0.18 seconds with 32 queries by YEKTAWEB 3977