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:: Volume 7, Issue 1 (1-2020) ::
JBRMS 2020, 7(1): 22-27 Back to browse issues page
Prevalence of mutations in V Leiden and prothrombin genes in women with recurrent pregnancy loss: A retrospective study on Iranian Azeri women
Elham Abbaszadeh , Seyyed Ali Rahmani , Shahla Danaei Mehrabad
Department of Molecular Biology, Ahar Branch, Islamic Azad University, Ahar, Iran , rahmaniseyedali@yahoo.com
Abstract:   (424 Views)
Introduction: The thrombophilia is one of the most important cause of maternal thromboembolism, which is associated with recurrent pregnancy loss (RPL) Risk. The aim of present study was to investigate prevalence of prothrombin (FII, G20210A) and factor V Leiden (FVL, G1691A) genes mutation, as two important cause of thrombophilia, in Iranian Azeri women with RPL.
Materials and methods: The subjects in this retrospective study consisted of 100 women (20-40 years old) with RPL recruited from Iranian Azeri population in East Azerbaijan province, Tabriz in Iran. The genomic DNA was extracted from 5 ml peripheral blood samples using the proteinase K method. The Allele and genotype of FII (G20210A) and FVL (G1691A) mutations were assessed using restriction fragment length polymorphism (RFLP) polymerase chain reaction (PCR) method.
Results: Our results showed that the frequency of normal homozygous, heterozygous and mutation homozygous for the FII G20210A and FVL G1691A mutations were equally distributed among Iranian Azeri women with RPL. The genotype distribution in RPL patients was, 99% AA, 1% AG, and 0% GG in both of the mutations.
Conclusion: In general, our study showed that the prevalence of FVL (G1691A) and FII (G20210A) mutations is low in the Iranian Azeri women with RPL. However, these mutations can be the important reasons for RPL, and more studies with large sample size are required to determine the exact frequency of FVL (G1691A) and FII (G20210A) mutations in Iranian Azeri women with RPL.
Introduction: The thrombophilia is one of the most important cause of maternal thromboembolism, which is associated with recurrent pregnancy loss (RPL) Risk. The aim of present study was to investigate prevalence of prothrombin (FII, G20210A) and factor V Leiden (FVL, G1691A) genes mutation, as two important cause of thrombophilia, in Iranian Azeri women with RPL.
Materials and methods: The subjects in this retrospective study consisted of 100 women (20-40 years old) with RPL recruited from Iranian Azeri population in East Azerbaijan province, Tabriz in Iran. The genomic DNA was extracted from 5 ml peripheral blood samples using the proteinase K method. The Allele and genotype of FII (G20210A) and FVL (G1691A) mutations were assessed using restriction fragment length polymorphism (RFLP) polymerase chain reaction (PCR) method.
Results: Our results showed that the frequency of normal homozygous, heterozygous and mutation homozygous for the FII G20210A and FVL G1691A mutations were equally distributed among Iranian Azeri women with RPL. The genotype distribution in RPL patients was, 99% AA, 1% AG, and 0% GG in both of the mutations.
Conclusion: In general, our study showed that the prevalence of FVL (G1691A) and FII (G20210A) mutations is low in the Iranian Azeri women with RPL. However, these mutations can be the important reasons for RPL, and more studies with large sample size are required to determine the exact frequency of FVL (G1691A) and FII (G20210A) mutations in Iranian Azeri women with RPL.
Keywords: Mutation, Factor V Leiden, Prothrombin, Recurrent pregnancy loss
Full-Text [PDF 469 kb]   (124 Downloads)    
Type of Study: Research | Subject: Genetics
Received: 2019/10/23 | Accepted: 2019/12/1 | Published: 2020/01/10
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Abbaszadeh E, Rahmani S A, Danaei Mehrabad S. Prevalence of mutations in V Leiden and prothrombin genes in women with recurrent pregnancy loss: A retrospective study on Iranian Azeri women. JBRMS. 2020; 7 (1) :22-27
URL: http://jbrms.medilam.ac.ir/article-1-461-en.html


Volume 7, Issue 1 (1-2020) Back to browse issues page
مجله ی تحقیقات پایه در علوم پزشکی Journal of Basic Research in Medical Sciences
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